The following antibodies were used in this study: mouse anti-Galectin-3 (BioLegend, 126702), goat anti-human GPNMB (R&D Systems, AF2550), goat anti-mouse GPNMB (R&D Systems, AF2330), rat anti-mouse LAMP1 (BD Biosciences, 553792), rabbit anti-IBA-1 (Wako, 01919741), goat anti-AIF-1/Iba1 (Novus Biologicals, NB100–1028), rat anti-CD68 (Bio-Rad, MCA1957), and sheep anti-PGRN (R&D Systems, AF2557), mouse anti-MBP (Millipore, SMI-99), Goat anti-Olig2 (R&D Systems, AF2418), mouse anti-APC (Millipore, OP80), rabbit anti-Perilipin2 (Proteintech Group,15294-1-AP), sheep anti-TREM2 (R&D Systems, AF1729). Detailed information is provided in Supplementary Table 1 .
The following reagents were also used in the study: Dulbecco’s modified Eagle’s medium (DMEM)(Cellgro, 10–017-CV), Hanks’ Balanced Salt Solution (HBSS) (Cellgro, 21–020-CV), Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F-12)(Cellgro, 10–092-CV), 0.25% Trypsin (Corning, 25–053-CI), Autofluorescence Quencher (Biotium, 23007), Odyssey blocking buffer (LI-COR Biosciences, 927–40000), and O.C.T compound (Electron Microscopy Sciences, 62550–01).
The following reagents were also used in the study: Dulbecco’s modified Eagle’s medium (DMEM)(Cellgro, 10–017-CV), Hanks’ Balanced Salt Solution (HBSS) (Cellgro, 21–020-CV), Dulbecco’s modified Eagle’s medium/Ham’s F-12 (DMEM/F-12)(Cellgro, 10–092-CV), 0.25% Trypsin (Corning, 25–053-CI), Autofluorescence Quencher (Biotium, 23007), Odyssey blocking buffer (LI-COR Biosciences, 927–40000), and O.C.T compound (Electron Microscopy Sciences, 62550–01).