Statistical analysis and graphing were conducted using the Origin Pro version 8.5 statistical software package (Origin Lab, Northampton, Massachusetts, USA). The experimental design was completely randomized, all experiments were performed in triplicate to increase the reliability of the results, and all data obtained in the physicochemical tests and the radial growth test was given a treatment in Excel software calculating averages and standard deviations. The enzyme activities were evaluated by analysis of variance (ANOVA), and the comparison of the means test was performed by Tukey's test using the Origin Pro.
Originpro version 8
Manufactured by OriginLab
Sourced in United States
OriginPro Version 8.5 is a data analysis and graphing software designed for scientific and engineering applications. It provides tools for data manipulation, curve fitting, and visualization of data in various formats.
Automatically generated - may contain errors
Lab products found in correlation
Spss for windows version 12, by IBM (2 mentions)
Spss software version 19, by IBM (1 mentions)
Agilent cary 660 ftir spectrophotometer, by Agilent Technologies (1 mentions)
1290 infinity uhplc system, by Agilent Technologies (1 mentions)
Udp glc, by Merck Group (1 mentions)
6540 uhd accurate mass q tof, by Agilent Technologies (1 mentions)
Axs d8 advance diffractometer, by Bruker (1 mentions)
Spss version 20, by IBM (1 mentions)
Statistica 10, by StatSoft (1 mentions)
Ds 11 spectrophotometer, by DeNovix (1 mentions)
Spss version 21, by IBM (1 mentions)
Version 18, by MedCalc (1 mentions)
Gladiatr, by PIKE Technologies (1 mentions)
Opus version 4, by Bruker (1 mentions)
Ft ir 4100 spectrometer, by Jasco (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Merck Group (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Olaparib, by Selleck Chemicals (1 mentions)
Whitley h35 hypoxystation, by Don Whitley Scientific (1 mentions)
36 protocols using originpro version 8
1
Comprehensive Statistical Analysis of Experimental Data
2
Antimicrobial Evaluation of Natural Compounds
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MIC and MBC values were expressed as the mode of at least three independent replicates. All other data are represented as the mean ± SD of three independent replicates. Analysis of variance (ANOVA) and the Tukey test were performed using OriginPro, version 8.5 (OriginLab Corporation, Northampton, MA, USA).
3
Electrospun Sample Degradation Kinetics
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To simulate physiological conditions, electrospun samples (n = 3) were individually incubated in a phosphate buffered solution (PBS, 10 mM, pH 7.4, 25 °C) [19 (link)] for up to 8 weeks, prior to the determination of any mass loss. DMPA photoinitiator-doped samples were also tested before and after curing to determine the change in the degradation behaviour due to the introduction of covalent crosslinks at the molecular scale. The samples were cut to equal dimensions (10 × 30 mm), and the initial mass of the dry samples was measured and then re-measured at 1-week intervals to quantify the percent residual mass. All mass measurements were obtained using oven-dried samples (dried at 50 °C for 24 h, in a Binder ED56 Series static oven, BINDER, Tuttlingen, Germany). The results were linearly fitted to assess erosion-driven degradability. The fitting of a linear model using OriginPro (version 8.5) was used to determine the fitting equations and the coefficient of determination (R2) for each degradation profile, allowing for an understanding of the relationship between the variables time and mass.
4
Bioactive Compound Extraction Optimization
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All data are expressed as the means ± standard deviation of three replicates. Statistical analyses were performed using OriginPro Version 8.5 software (OriginLab Corporation, Northampton, MA, USA). Pearson's correlation coefficients and one-way analysis of variance were performed using SPSS 13.0 (SPSS Inc., Chicago, IL, USA) to identify differences between samples; P < 0.05 was considered statistically significant.[15 (link)]
5
Statistical Analysis of Experimental Data
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All the tests in this study were conducted at least in triplicate. The data were expressed as average plus standard deviation (SD). The SPSS software (version 19.0) was used for one-way analysis of variance (ANOVA). Significant differences were separated using Duncan's multiple range test, with p < 0.05 as the level of significance. OriginPro version 8.5 software was used to produce the graphs.
6
Characterizing Chitin and Chitosan Crystallinity
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X-ray diffraction was used to determine the crystallinity of the isolated chitin and chitosan where 500 mg of each sample chitosan powder were analyzed employing BRUKER AXS diffractometer, D8 Advance (Germany) fitted with Cu-Kα radiation (λKα1 = 1.5406 Å) from 2θ = 0.5° to 130°, with increment ∆2ϑ: (0.034°), voltage of 40 kV, current of 40 mA, power of 1.6 kW and counting time of 0.5 s/step. Generated data was analyzed by OriginPro Version 8.5 and resultant peaks 2θ values were compared with the commercial shrimp chitosan from Sigma Aldrich. The crystalline Index (CrI) values were determined from the XRD pattern following Focher et al.59 methods using formula 9 .
where I200 is the maximum peak intensity for each chitosan at 2Ɵ–20° and Iam is the intensity of amorphous diffraction at 2Ɵ–16°.
where I200 is the maximum peak intensity for each chitosan at 2Ɵ–20° and Iam is the intensity of amorphous diffraction at 2Ɵ–16°.
7
FTIR Analysis of Modified Starch Characteristics
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Fourier transform infrared spectroscopy (FTIR) was used to identify the characteristic functional groups present in the modified starch as well as to provide an estimate of degree of substitution (Machell & Richards) by the ratio of the area of peaks at around 1020 cm−1 (C-O-C vibration of glucose units) and 1730-1750 cm−1 (C=O vibration of ester group) in the modified starch spectrum. 5 mg of a starch sample was mixed with 195 mg of dry KBr and pressed to form a pellet before measurement by a Fourier transform infrared spectrometer (Agilent Cary 660 FT-IR Spectrophotometer, Agilent, USA). The samples were scanned from 4000 to 800 cm−1, averaged over 64 scans with a resolution of 4 cm−1. Spectra were analyzed and processed with OriginPro version 8.5 (OriginLab, USA) and Microsoft Excel.
8
Statistical Analysis of Comet Assay
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Statistical analysis of data from the comet assay was performed using one-way ANOVA (OriginPro, version 8.5, Stoke Mandeville, UK). p value at < 0.05 was considered to be statistically significant. Statistical analysis of data in MN, apoptosis and mitotic spindle study was achieved by the G-test for independence on 2 × 2 tables. This test is based on the general assumption of the χ2 analysis, but offers theoretical and computational advantages.
9
Kinetic Analysis of UGT76B1 Substrates
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UGT76B1 recombinant protein was incubated with substrates NHP, SA, and ILA for 30 min at 30°C. The reaction was stopped by the addition of 20% acetonitrile. Samples were analyzed using a 1290 Infinity UHPLC system coupled to a 6540 UHD Accurate-Mass Q-TOF (Agilent Technologies, Santa Clara, CA, USA) as previously described (Feussner and Feussner, 2019 ). Kinetic parameters of UGT76B1’s substrates NHP, SA, and ILA were analyzed as described under UPLC-nanoESI-QTRAP-MS-based metabolite quantification. The reaction mixture contained 3.5-µg UGT76B1, 2 mM UDP-Glc (Merck, Darmstadt, Germany), and 0–2.5 mM substrate. Before incubation with UGT76B1, the initial amount of substrate was determined for analysis of substrate reduction. The reaction was incubated for 15 min at 30°C and stopped by the addition of methanol. The difference in signal intensity of substrate was plotted for each substrate and concentration. The KM was determined via Hill regression analysis using OriginPro version 8.5 (OriginLab Corporation, Northampton, MA, USA).
10
Chitosan Crystallinity by X-Ray Diffraction
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X-Ray diffraction was used to determine the crystallinity of the isolated chitosan where 500mg of chitosan powder were analyzed employing BRUKER AXS diffractometer, D8 Advance (Germany) fitted with Cu-Kα radiation (λKα1=1.5406Å) from 2θ = 0.5° to 130°, with increment D2J: (0.034°), voltage of 40 kV, current of 40 mA, power of 1.6 kW and counting time of 0.5 sec/step. Generated data was analyzed by OriginPro Version 8.5 and resultant peaks 2θ values were compared with the commercial shrimp chitosan from Sigma Aldrich.
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