Biomek i7

Manufactured by Roche

The Biomek i7 is an automated liquid handling workstation designed for a wide range of laboratory applications. It features a high-precision pipetting system and advanced software control, enabling efficient and accurate liquid handling tasks.

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Lab products found in correlation

Quick rna kit, by Zymo Research (3 mentions) Ercc exfold rna spike in mix, by Thermo Fisher Scientific (2 mentions) Kapa mrna hyperprep strand specific sample preparation kits, by Roche (2 mentions)

3 protocols using biomek i7

RNA-seq With ERCC Spike-ins

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For RNA-seq experiments, the total cellular RNA was extracted using the QuickRNA kit (Zymo Research R1054). Purified total RNA was mixed with the ERCC ExFold RNA spike-in mix (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid-handling platform using Roche Kapa mRNA HyperPrep strand-specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer's protocol. Library quantification and Illumina sequencing were performed as described in the ChIP-seq section above.

Harada T., Heshmati Y., Kalfon J., Perez M.W., Xavier Ferrucio J., Ewers J., Hubbell Engler B., Kossenkov A., Ellegast J.M., Yi J.S., Bowker A., Zhu Q., Eagle K., Liu T., Kai Y., Dempster J.M., Kugener G., Wickramasinghe J., Herbert Z.T., Li C.H., Vrabič Koren J., Weinstock D.M., Paralkar V.R., Nabet B., Lin C.Y., Dharia N.V., Stegmaier K., Orkin S.H, & Pimkin M. (2022). A distinct core regulatory module enforces oncogene expression in KMT2A-rearranged leukemia. Genes & Development, 36(5-6), 368-389.

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Bulk RNA-seq with ERCC Spike-ins

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For RNA-seq experiments, the total cellular RNA was extracted using the QuickRNA kit (Zymo Research R1054). Purified total RNA was mixed with the ERCC synthetic spike-in control (Invitrogen 4456740). RNA-seq libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand-specific sample preparation kits (Roche; 08098123702) from 200 ng of purified total RNA according to the manufacturer's protocol. Library quantification and Illumina sequencing were performed as described in the ChIP-seq section above.

Eagle K., Harada T., Kalfon J., Perez M.W., Heshmati Y., Ewers J., Koren J.V., Dempster J.M., Kugener G., Paralkar V.R., Lin C.Y., Dharia N.V., Stegmaier K., Orkin S.H, & Pimkin M. (2022). Transcriptional Plasticity Drives Leukemia Immune Escape. Blood Cancer Discovery, 3(5), 394-409.

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RNA-seq with ERCC Spike-in

Check if the same lab product or an alternative is used in the 5 most similar protocols

For RNA-seq experiments the total cellular RNA was extracted using the QuickRNA kit (Zymo Research R1054). Purified total RNA was mixed with the ERCC ExFold RNA Spike-in mix (Invitrogen 4456740). RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol. Library quantification and Illumina sequencing were performed as described in the ChIP-seq section above.

Harada T., Kalfon J., Perez M.W., Eagle K., Braes F.D., Batley R., Heshmati Y., Ferrucio J.X., Ewers J., Mehta S., Kossenkov A., Ellegast J.M., Bowker A., Wickramasinghe J., Nabet B., Paralkar V.R., Dharia N.V., Stegmaier K., Orkin S.H, & Pimkin M. (2023). Leukemia core transcriptional circuitry is a sparsely interconnected hierarchy stabilized by incoherent feed-forward loops. bioRxiv.

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