The murine RAW264.7 macrophage cell line was purchased from the China Center for Type Culture Collection (CCTCC, China). The RAW264.7 macrophages were seeded in 60-mm cell culture dishes at 1.0×106 cells per dish in DMEM containing 10% fetal bovine serum and maintained at 37°C in a humidified atmosphere of 5% CO2. The cells were synchronized by replacing the medium with DMEM supplemented with 0.5% BSA for 12 h before the experiments. After 12h, the cells were transfected with PBS or active ingredient of drugs (Final concentration about 25 μmol/L) for 48 h. Quercetin, luteolin, kaempferol, naringenin, tanshinone IIA, β-carotene, 7-O-methylisomucronulatol, piperine, isorhamnetin and Xyloidone were purchased commercially (Solarbio, Beijing, China).
Kaempferol
Manufactured by Solarbio
Sourced in China
Kaempferol is a naturally occurring flavonoid compound. It is used as a research tool in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Quercetin, by Solarbio (4 mentions)
Luteolin, by Solarbio (4 mentions)
Rutin, by Solarbio (4 mentions)
Naringenin, by Solarbio (2 mentions)
Chlorogenic acid, by Solarbio (2 mentions)
Apigenin, by Solarbio (2 mentions)
Gallic acid, by Solarbio (2 mentions)
β carotene, by Solarbio (1 mentions)
Superoxide dismutase, by Solarbio (1 mentions)
Malondialdehyde, by Solarbio (1 mentions)
Bca protein quantitation kit, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cck 8, by Solarbio (1 mentions)
Penicillin streptomycin solution, by ScienCell (1 mentions)
Hyperoside, by Solarbio (1 mentions)
Cyanidin 3 o glucoside, by Solarbio (1 mentions)
Cyanidin 3 o galactoside, by Solarbio (1 mentions)
Catechin, by Solarbio (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by Solarbio (1 mentions)
11 protocols using kaempferol
1
Murine Macrophage Cell Culture and Treatment
2
Evaluating Antioxidant Potential of Kaempferol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Kaempferol, AAPH, superoxide dismutase (SOD), malondialdehyde (MDA), and cytotoxicity assay (CCK-8) kits were obtained from Solarbio (Beijing, China). 3,5-dimethylphenol was obtained from Life Technologies (Carlsbad, CA, United States). Phosphate-buffered saline (PBS) was procured from Biological Industries (Ridgefield, CT, United States). Penicillin–streptomycin solution was obtained from ScienCell (San Diego, CA, United States). Dulbecco’s modified Eagle’s medium (DMEM) and trypticase were purchased from HyClone (Logan, UT, United States). Fetal bovine serum (FBS) was procured from Gibco (Waltham, MA, United States). A BCA Protein Quantitation Kit was obtained from Thermo Fisher Scientific (Waltham, MA, United States).
3
Flavonoid Profiling of Chinese Prickly Ash Peels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 26 Chinese prickly ash peels materials were collected from eight provinces of China (the main production areas: Guide, Xunhua, Hanyuan, Jiuzhaigou, Wenxian, Wudu, Qin’an, Fengxian, Fuping, Hengshan, Hancheng, Yongji, Lingbao, Jiaocheng, Shexian, Zaozhaung and Laiwu) at different altitudes (201–2188 m) from July to August 2020 (Figure 1 ). The 26 samples were divided into 6 groups based on the geographical location and altitude of the sample collection sites, as shown in Table S1 . Under the premise of protecting local germplasm resources, Chinese prickly ash mature fruits from each location were collected in three replicates, and the distance between each plant was more than 50 m. Fruits without pests and mechanical damage were dried in the laboratory at room temperature until they reached a constant weight.
A total of 15 flavonoid compound standards (hyperoside, luteolin, kaempferol, quercitrin, catechin, rutin, chlorogenic acid, quercetin, hesperetin, apigenin, peonidin O-hexoside, peonidin 3-O-glucoside, cyanidin 3-O-glucoside, cyanidin O-syringic acid, cyanidin 3-O-galactoside) were bought (Beijing Solarbio Science & Technology, Beijing, China). HPLC-grade methanol, acetic acid and acetonitrile were bought (TEDIA Chemical Co., Ltd., Fairfield, OH, USA). Deionized water (18 MΩ cm) was used to prepare aqueous solutions (MilH-Q Advantage A1, Millipore, Billerica, MA, USA).
A total of 15 flavonoid compound standards (hyperoside, luteolin, kaempferol, quercitrin, catechin, rutin, chlorogenic acid, quercetin, hesperetin, apigenin, peonidin O-hexoside, peonidin 3-O-glucoside, cyanidin 3-O-glucoside, cyanidin O-syringic acid, cyanidin 3-O-galactoside) were bought (Beijing Solarbio Science & Technology, Beijing, China). HPLC-grade methanol, acetic acid and acetonitrile were bought (TEDIA Chemical Co., Ltd., Fairfield, OH, USA). Deionized water (18 MΩ cm) was used to prepare aqueous solutions (MilH-Q Advantage A1, Millipore, Billerica, MA, USA).
4
Chemical Reagents Procurement for Analytical Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hexane and methanol were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China and ethyl acetate and ethanol from Hunan Huihong Reagent Co., Ltd. (Hunan, China). Folin-Ciocalteu reagent and vanillin were purchased from Coolaber Science and Technology, Beijing, China. DPPH, ABTS, and T-AOC (FRAP), apigenin, (+)-catechin, gallic acid, glucose, kaempferol, quercetin, luteolin, naringenin, and rutin, were all purchased from Solarbio Life Sciences, Beijing, China. HPLC grade acetic acid and acetonitrile were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China).
5
Kaempferol Cytotoxicity Assay in Ishikawa Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Kaempferol was purchased from Solarbio Science &Technology Co.,Ltd (Beijing, China) [18 (link)]. The purity of Kaempferol used in the following experiments was>98%. Kaempferol was dissolved in DMSO at a concentration of 200 mmol/L.
Ishikawa cells were seeded in 96-well plates at a density of 5,000 cells per well and cultured in a complete cell culture medium for 24 h for recovery. Next, the medium was changed to DMEM with 0, 25, 50, 75, 100, 150, and 200 µmol/L Kaempferol, without FBS, and cultured for 48 h. Then, 10 μL of sterile Cell Counting Kit (CCK)8 solution (Bimake, USA) was added to each well and incubated for another 1.5 h at 37 °C. Absorbance was measured at 450 nm using a microplate reader.
Ishikawa cells were seeded in 96-well plates at a density of 5,000 cells per well and cultured in a complete cell culture medium for 24 h for recovery. Next, the medium was changed to DMEM with 0, 25, 50, 75, 100, 150, and 200 µmol/L Kaempferol, without FBS, and cultured for 48 h. Then, 10 μL of sterile Cell Counting Kit (CCK)8 solution (Bimake, USA) was added to each well and incubated for another 1.5 h at 37 °C. Absorbance was measured at 450 nm using a microplate reader.
6
Quantification of Bioactive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
xanthine oxidase, xanthine, and standard substances (≥98%) including chlorogenic acid, gallic acid, quercetin-3-O-rhamnoside, kaempferol, quercetin, kaempferitrin, engeletin, iso-quercetin, astragalin, and rutin were bought from Beijing Solarbio Technology Co., Ltd. (Beijing, China). Allopurinol was supplied by Shanghai Xinyi Wanxiang Pharmaceutical Co., Ltd. (Shanghai, China). A laboratory mill was purchased from Zhejiang Rhodiola Industry and Trade Co., Ltd (DE-1000gA, Jinhua, China). The qualitative analysis of compounds was completed by HPLC analysis on an Agilent 1260 HPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with a C18 reverse-phase column.
7
Isolation and Characterization of Bioactive Compounds from Syringa oblata
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Syringa oblata Lindl. was growing naturally on the campus of Northeast Agricultural University (N 45°44′33.64″, E 126°43′22.07″) in Harbin, Heilongjiang Province of China, and were authenticated by Professor Xiuju Wu from College of Life Sciences, Northeast Agricultural University. The raw materials were dried, pulverized, sifted through an 80-mesh sieve, and stored at 4°C before use. Voucher specimens were deposited at the Department of Animal Pharmacy, Northeast Agricultural University. Rutin, luteolin, oleanolic acid, and kaempferol standards (purity≥98%) were purchased from the Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). L-proline, L-lysine, 1,2-propanediol, glycerol, glycol, 1,4-butanediol, methanol, and other chemicals were obtained from the Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). D101 macroporous resin was procured from the Tianjin BSF Resin Technology Co. Ltd. (Tianjin, China).
8
Kaempferol's Impact on Diamondback Moth
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Kaempferol is commonly found in high abundance in cruciferous vegetables and can play an important protective role in plants against Spodoptera litura attack (35 (link)– (link)37 (link)). In this study, it was selected to further identify its interaction with DBM and gut bacteria. Kaempferol (Solarbio, China) was solubilized in methanol and added to the germ-free artificial diet to make the final concentration 0.1 mg/mL. Thirty first instar DBM larvae were randomly selected to feed on this artificial diet, and another 30 were used to feed on the artificial diet with the same volume of solvent as CK. The fourth instar larval weight and the larval development time were recorded, and the experiment was run three times. Data were analyzed using a one-way ANOVA in IBM SPSS 19 software.
9
Antioxidant Compound Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dimethyl sulfoxide (DMSO), 2′,7′-dichlorfluorescin diacetate (DCFH–DA), and 2,2-azobis (2-amidinopropane) dihydrochloride solution (ABAP) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Epigallocatechin gallate (EGCG, HPLC grade, purity > 98%) and kaempferol (HPLC grade, purity > 98%) were obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). EGCG and kaempferol were dissolved in dimethyl sulfoxide (DMSO) at a concentration of 10 mg/mL and then freshly diluted in culture medium. The final concentration of DMSO in culture medium was below 0.05% (v/v). Phosphate buffer solution (PBS), minimum Eagle’s medium (MEM), fetal bovine serum (FBS), 0.05% trypsin–EDTA solution, penicillin (10,000 u/mL), and streptomycin (10,000 μg/mL) were purchased from HyClone (Logan, UT, USA). The Cell Counting Kit-8 (CCK-8) was obtained from Dojindo China Co., Ltd. (Shanghai, China). The BCA protein concentration test kit; cell lysis buffer for Western and IP; and phenylmethanesulfonyl fluoride (PMSF, 100 mM), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) assay kits were purchased from Beyotime Biotechnology (Shanghai, China). All other chemicals and reagents with analytical grade used in this study were obtained from Sinopharm Group Chemical Reagents Co. Ltd. (Beijing, China).
10
Cisplatin-Induced Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cisplatin was purchased from Aladdin Industrial Corporation (Shanghai, China). AO (acridine orange), EB (ethidium bromide), MTT (3-[4, 5-dimethylth-iazol-2-yl]-2, 5 diphenyl tetrazolium bromide), BCA protein assay kit, hesperetin, kaempferol, and 4% paraformaldehyde solution were from Solarbio Life Sciences (Beijing, China). Crystal violet staining solution, Hoechst 33,258, RIPA lysis buffer, mitochondrial membrane potential assay kit with JC-1, and BeyoECL moon kit were purchased from Beyotime Institute of Biotechnology (Shanghai, China). Antibodies were from Cell Signaling Technology (Danvers, Massachusetts, United States).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!