H3k56ac ab76307

Recombinant p300 (1195–1662) was from Enzo and pCAF (503–668) was obtained from Caymen Chemical. (R)- and (S)-ibuprofen and celecoxib were purchased from Santa Cruz. Haloxyfop was purchased from Fluka. Salicylic acid, racemic ibuprofen, naproxen, ketoprofen, diclofenac, 5-fluoroSalicylic acid, phenylacetic acid, benzoic acid and sodium acetate were purchased from Sigma. All purchased chemicals were used without further purification unless otherwise noted. All synthesized CoA analogues were HPLC purified, with purity verified by LC-MS prior to use, and concentrations were measured by UV analysis on a Thermo-Fisher Nanodrop 2000 spectrophotometer in distilled and deionized water (ddH₂O) using the molar extinction coefficient (ε) for Coenzyme A of 15,000 M⁻¹cm⁻¹ at λ_max of 259 nm. Qubit Protein Assay kit (Life Technologies) was used to determine histone extract concentrations. Labchip EZ-Reader 12-sipper chip (#760404) and ProfilerPro Separation Buffer (#760367) were purchased from Perkin-Elmer. Antibodies¹¹ used are as follows: H3K9Ac (9649P), H3K14Ac (7627P), H3K27Ac (8173) antibodies were purchased from Cell Signaling Technologies. The H2B K12/K15Ac (ab1759), total H2B (ab1790), and H3K56Ac (ab76307) antibodies were purchased from abcam. The pan-H4Ac (06–866) and pan-H3Ac (07–690) antibodies were purchased from Millipore Sigma.