Fluorescein isothiocyanate fitc labelled human insulin

Chitosan (Average Mw 100 KDa, 75–85% deacetylated) was purchased from Sigma-Aldrich. (Oakville, Ontario, Canada). Sodium tripolyphosphate (TPP) was purchased from VWR (Radnor, Pennsylvania, USA). Recombinant human insulin used in this study was from Fisher Scientific (Waltham, Massachusetts, USA). Fluorescein isothiocyanate (FITC)-labelled human insulin and the 4’, 6-Diamidino-2-phenylindole dihydrochloride (DAPI) were purchased from Sigma-Aldrich. (Oakville, Ontario, Canada). The HepG2 cell line was obtained from ATCC (Manassas, Virginia, USA). All the other reagents were of analytical or chromatography grade.

Chitosan (Average Mw 100 KDa, 75–85% deacetylated), Carbopol 934P (viscosity 800–11,000 cP), hydroxypropyl methylcellulose (viscosity 2600–5600 cP), and ethylcellulose (viscosity 10 cP) were purchased from Sigma-Aldrich. (Oakville, Ontario, Canada). Sodium tripolyphosphate (TPP) and sodium alginate (low viscosity) was purchased from VWR (Radnor, Pennsylvania, USA). Recombinant human insulin was obtained from Fisher Scientific (Waltham, Massachusetts, USA). Fluorescein isothiocyanate (FITC)-labelled human insulin and the 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Sigma-Aldrich. (Oakville, Ontario, Canada). The HepG2 and Caco-2 cell line was obtained from ATCC (Manassas, Virginia, USA) while TR-146 Cell line was purchased from Sigma-Aldrich. (Oakville, Ontario, Canada). Other reagents were of analytical or chromatography grade.