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3 4 5dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt kit

Manufactured by Beyotime
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3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit is a colorimetric assay used for measuring the activity of enzymes that reduce MTT to formazan, thereby indicating the level of cellular viability and proliferation in cell cultures.

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Lab products found in correlation

Mirna mimics and inhibitor, by GenePharma (2 mentions) P53 antibody, by Beyotime (2 mentions) Sybr green polymerase chain reaction kit, by Selleck Chemicals (2 mentions) Triton x, by Vetec (2 mentions) Dualluciferase reporter vector plasmid, by GenePharma (2 mentions) Dual luciferase reporter gene uorescence detection kit, by Promega (2 mentions) Dulbecco s modi ed eagle medium dmem f12, by Thermo Fisher Scientific (2 mentions) Goat anti mouse igg horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (2 mentions) Revertaid first strand complementary dna cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Trizol, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Sartorius (2 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Signalway Antibody (2 mentions) Crystal violet, by Beyotime (2 mentions) Cell counting kit 8 (cck8), by Beyotime (2 mentions) Trypsin, by Beyotime (2 mentions) Murine il 4, by Thermo Fisher Scientific (1 mentions) Live dead baclight bacterial viability kit, by Thermo Fisher Scientific (1 mentions) Murine granulocyte macrophage colony stimulating factor gm csf, by Thermo Fisher Scientific (1 mentions) Human tnf α, by Thermo Fisher Scientific (1 mentions)

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MTT kit (73 citations)

3 protocols using 3 4 5dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt kit

1

Antimicrobial and Immunomodulatory Nanoparticle Formulation

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GMS and Nor were obtained from Aladdin, Shanghai, China. Poloxamer 188 was obtained from Beijing Probe Bioscience Co. Ltd., Beijing, China. Bicinchoninic acid assay kit, SDS–polyacrylamide gel electrophoresis (PAGE) kit, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, DiI, and DiO were obtained from Beyotime Biotechnology, Shanghai, China. Mueller-Hinton broth (MHB) and agar were obtained from BD Difco, USA. RPMI 1640 cell culture medium was obtained from Gibco, Thermo Fisher Scientific, USA. IL-6 and TNF-α enzyme-linked immunosorbent assay (ELISA) kits were obtained from NeoBioscience Co. Ltd., Shenzhen, China. LIVE/DEAD BacLight bacterial viability kit (L-7012) was bought from Invitrogen, Thermo Fisher Scientific, USA. Fluorescein isothiocyanate (FITC)–conjugated anti-mouse CD11c, phycoerythrin (PE)–conjugated anti-mouse CD80, PE-conjugated anti-mouse CD86, allophycocyanin (APC)–conjugated anti-mouse CD3, PE-conjugated anti-mouse CD8a, and FITC-conjugated anti-mouse CD4 were purchased from Tonbo Biosciences, USA. Murine IL-4, murine granulocyte-macrophage colony-stimulating factor (GM-CSF), and human TNF-α were bought from PeproTech, USA. E. coli (ATCC no. 25922), S. aureus (ATCC no. 6538), K. pneumoniae (ATCC no. 4352), and HL-60 (ATCC no. CCL-240) were obtained from the American Type Culture Collection (ATCC).
Peng X., Chen J., Gan Y., Yang L., Luo Y., Bu C., Huang Y., Chen X., Tan J., Yang Y.Y., Yuan P, & Ding X. (2024). Biofunctional lipid nanoparticles for precision treatment and prophylaxis of bacterial infections. Science Advances, 10(14), eadk9754.
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2

Evaluating miRNA Regulatory Mechanisms

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PTX was purchased from Aladdin. Dulbecco's Modi ed Eagle Medium (DMEM)/F12 was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Biological Industries. Trypsin, crystal violet, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, Cell Counting Kit-8, and P53 antibody were purchased from Beyotime Biotechnology. Trizol was purchased from Ambion. RevertAid First Strand complementary DNA (cDNA) Synthesis Kit was purchased from Thermo. SYBR Green Polymerase Chain Reaction (PCR) kit was purchased from Bimake. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Signalway Antibody. Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody was purchased from Santa Cruz Biotechnology. MDM2 antibody was purchased from A nity. Polyvinylidene di uoride (PVDF) membrane was purchased from Immobilon. 0.3% triton-X was purchased from Vetec. Lipofectamine®3000 transfection reagent was purchased from Invitrogen. MiRNA mimics and inhibitors were synthesized by GenePharma (Shanghai, China). Dualluciferase reporter vector plasmid was purchased by GenePharma (Shanghai, China). Dual-luciferase reporter gene uorescence detection kit was purchased by Promega.
Ni L., Xu J., Zhao F., Dai X., Tao J., Pan J., Shi A., Shen Z., Su C., & Zhang Y. (2020). MiR-221-3p-Mediated Downregulation of MDM2 Reverses the Paclitaxel Resistance of Non-Small Cell Lung Cancer in Vitro and in Vivo.
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3

Evaluating miRNA Regulatory Mechanisms

Check if the same lab product or an alternative is used in the 5 most similar protocols
PTX was purchased from Aladdin. Dulbecco's Modi ed Eagle Medium (DMEM)/F12 was purchased from Gibco. Fetal bovine serum (FBS) was purchased from Biological Industries. Trypsin, crystal violet, 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit, Cell Counting Kit-8, and P53 antibody were purchased from Beyotime Biotechnology. Trizol was purchased from Ambion. RevertAid First Strand complementary DNA (cDNA) Synthesis Kit was purchased from Thermo. SYBR Green Polymerase Chain Reaction (PCR) kit was purchased from Bimake. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from Signalway Antibody. Goat anti-mouse IgG horseradish peroxidase (HRP)conjugated secondary antibody was purchased from Santa Cruz Biotechnology. MDM2 antibody was purchased from A nity. Polyvinylidene di uoride (PVDF) membrane was purchased from Immobilon. 0.3% triton-X was purchased from Vetec. Lipofectamine®3000 transfection reagent was purchased from Invitrogen. MiRNA mimics and inhibitors were synthesized by GenePharma (Shanghai, China). Dualluciferase reporter vector plasmid was purchased by GenePharma (Shanghai, China). Dual-luciferase reporter gene uorescence detection kit was purchased by Promega.
Ni L., Xu J., Zhao F., Dai X., Tao J., Pan J., Shi A., Shen Z., Su C, & Zhang Y. (2021). MiR-221-3p-mediated downregulation of MDM2 reverses the paclitaxel resistance of non-small cell lung cancer in vitro and in vivo. European journal of pharmacology, 899.
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