To conduct the differential counting of rumen-ciliated protozoa, ruminal contents were manually collected by sweeping the floor of the rumen. Subsequently, 10 mL of this material was preserved in a vial containing 20 mL of 50% (v / v) formaldehyde. Collections were carried out on days 20 and 27 of each experimental period, 4 h after the morning feeding. For analysis, a 1-mL sample was mixed with two drops of 2% brilliant green dye (Sigma, B6756) and diluted with 9 mL of 30% glycerol. Protozoa, including those of the genera Isotricha, Dasytricha, Entodinium, and Diplodiniinae subfamily, were identified and counted using a Sedgwick counting chamber Rafter with internal dimensions of 50 mm × 20 mm × 1 mm (capacity 1 mL) through optical microscopy (Olympus CH-2®, Japan) (41 ).
Brilliant green dye
Manufactured by Merck Group
Sourced in United States
Brilliant green dye is a synthetic dye used in various laboratory applications. It is a crystalline solid with a characteristic green color. The dye is soluble in water and has a range of uses in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Nitric acid, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Ethylene glycol, by Merck Group (1 mentions)
Aniline, by Merck Group (1 mentions)
Hydrazine hydrate, by Merck Group (1 mentions)
2 naphthalenesulfonic acid, by Merck Group (1 mentions)
Benzoquinone, by Merck Group (1 mentions)
T butanol, by Merck Group (1 mentions)
Nickel 2 chloride hexahydrate, by Merck Group (1 mentions)
2 protocols using brilliant green dye
1
Differential Rumen Ciliate Protozoa Counting
2
PANI/Ni0 Nanocomposite Synthesis
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The reagents including aniline (ANI, 99%,), monomer for PANI polymer, 2naphthalene sulfonic acid (2-NSA, 70%) used as dopant, ammonium persulfate (APS, 98% purity), the oxidant for polymerization were purchased from Sigma Aldrich, USA. Nickel(II) chloride hexahydrate, precursor for Ni 0 nanoparticles, the reducing agent hydrazine hydrate (50-60%), ethylene glycol (EG), the medium for PANI/Ni 0 synthesis, brilliant green dye (dye content: 90%), sodium hydroxide, nitric acid, methanol, benzoquinone, t-butanol and all other chemicals used were also acquired from Sigma Aldrich, USA.
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