After completing the treatments, blood samples were gathered and subjected to centrifugation at a speed of 2500 rpm at 4°C for a duration of 30 min. Afterwards, the liquid above was meticulously transferred to cryogenic vials and promptly preserved at -80°C for subsequent examination. According to the manufacturer’s instructions, the levels of testosterone (CEA458Ge, Cloud-Clone Corp., China), luteinizing hormone (LH) (CEA441Mu, Cloud-Clone Corp., China), and insulin (80-INSMSU-E01, ALPCO, USA) underwent detection through utilizing enzyme-linked immunosorbent assay (ELISA) kits.
Cea458ge
Manufactured by Cloud-Clone
Sourced in China
The CEA458Ge is a laboratory equipment product designed for general scientific applications. It serves as a core functional component without interpreting or extrapolating on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cea458ge
1
Hormonal Analysis of Blood Samples
2
Quantification of Testicular Testosterone
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Concentrations of testosterone in supernatant of testicular organoids were quantified by ELISA according to the manufacturer's instructions. Testosterone reagents were purchased from Cloud-Clone (CEA458Ge, Cloud-Clone, China). Briefly, 100 μL standard or testis tissue supernates (100 μL/well) was added, then plate was incubated for 1 h at 37 °C. Subsequently, 50 μL Detection Reagent A was added and the plate was incubated for 1 h at 37 °C. After washing, 50 μL Detection Reagent B was added and incubated for 30 min. Finally, 90 μL Substrate Solution was added and incubated at 37 °C for 15 min, followed by addition of 50 μL Stop Solution. The absorbance was measured at 450 nm in a Multiskan spectrum 1500 (Thermo, USA).
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