Transient siRNA experiments were performed using HiPerfect Transfection Reagent (Qiagen, Hilden, North Rhine-Westphalia, Germany). The following siRNA oligos were transfected at 50nM final concentration, using the manufacturer’s guidelines (Dharmacon, Lafayette, CO, USA): The siRNA sequences used were the following: a) Human ON-TARGETplus SMARTpools against RAD51 (Dharmacon, L-003530-00-005), which contains these 4 oligonucleotides: 5’-UAUCAUCGCCCAUGCAUCA-3’ , 5’-CUAAUCAGGUGGUAGCUCA-3’ , 5’-GCAGUGAUGUCCUGGAUAA-3’ , 5’-CCAACGAUGUGAAGAAAUU-3’ ; b) Human ON-TARGETplus non-targeting pool (Dharmacon, D-001810-10-20) was used as a control containing these 4 oligonucleotides: 5’-UGGUUUACAUGUCGACUAA-3’ , 5’-UGGUUUACAUGUUGUGUGA-3’ , 5’-UGGUUUACAUGUUUU CUGA-3’ , 5’-UGGUUUACAUGUUUUCCUA-3’ .
Human on targetplus non targeting pool
Manufactured by Horizon Discovery
Sourced in United States
The Human ON-TARGETplus non-targeting pool is a laboratory product designed for conducting RNA interference (RNAi) experiments. It serves as a control for assessing off-target effects in RNAi studies. The pool contains a mixture of small interfering RNAs (siRNAs) that do not target any known human gene.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human on targetplus non targeting pool
1
Transient siRNA Knockdown of RAD51
2
Transient Rad51 Knockdown using siRNA
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Transient siRNA experiments were performed using HiPerfect Transfection Reagent (Qiagen, Hilden, North Rhine-Westphalia, Germany). The following siRNA oligos were transfected at 50nM final concentration, using the manufacturer's guidelines (Dharmacon, Lafayette, CO, USA): The siRNA sequences used were the following: a) Human ON-TARGETplus SMARTpools against Rad51 (Dharmacon, L-003530-00-005), which contains these 4 oligonucleotides: 5'-UAUCAUCGCCCAUGCAUCA-3', 5'-CUAAUCAGGUGGUAGCUCA-3', 5'-GCAGUGAUGUCCUGGAUAA-3', 5'-CCAACGAUGUGAAGAAAUU-3'; b) Human ON-TARGETplus non-targeting pool (Dharmacon, D-001810-10-20) was used as a control containing these 4 oligonucleotides: 5'-UGGUUUACAUGUCGACUAA-3', 5'-UGGUUUACAUGUUGUGUGA-3', 5'-UGGUUUACAUGUUUU CUGA-3', 5'-UGGUUUACAUGUUUUCCUA-3'.
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