Cell viability was examined using the Cell Counting Kit-8 (CCK-8) method, using a commercialized kit (Beyotime, China). As per the manufacturer’s introduction, treated cell lines were seeded in the 96-well plate containing the DMEM culture medium at a density of 2×105 cells/well and were incubated at 37°C for 24 hours in a CO2 incubator. After the cultivation, CCK-8 reagent (10 μL) was added into each plate well of corresponding groups and thoroughly mixed, followed by another 1-hour incubation. The absorbance was finally examined at 450 nm using a microplate reader (µQuant MQX200, Bio-Tek Instruments, USA).
Quant mqx200
Manufactured by Agilent Technologies
Sourced in United States
The µQuant MQX200 is a microplate spectrophotometer designed for accurate and reliable absorbance measurements. It features a compact design and supports 6- to 96-well microplates. The instrument utilizes a xenon flash lamp as the light source and employs dual-beam optics to provide precise and stable measurements.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using quant mqx200
1
Cell Viability Evaluation via CCK-8
2
Cytotoxicity Evaluation of Rg3 on Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The Cell Counting Kit-8 (CCK-8, Dojindo Labratories, Kumamoto, Japan) was used to measure the cytotoxicity of Rg3 on LLC and B16F10 cells. The cells (2×103 cells/well) were cultured in the presence of Rg3 (100, 200, 300, 400, 500 µg/ml) for 48 h in 96-well plates at 37℃ in a 5% CO2 incubator. After 10 µl CCK-8 solution was added to each well, the plate was re-incubated for 3 h at 37℃ and the absorbance at 450 nm was detected using a microplate reader (µ Quant MQX200; Bio-Tek, Winooski, Vermont, USA).
3
Protein Content Determination of Enzyme Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protein content of the crude enzyme extracts was determined using an assay kit (Bio-Rad Protein Assay) adapted from Bradford [29 (link)] with bovine serum albumin (BSA) as an external standard. Bovine serum albumin (BSA) concentrations for the calibration curve were in the range of 10 to 75 µg/mL. Aliquots of 50 µL of standard solution or enzyme extract were pipetted into a well of a polystyrene microplate. Subsequently, 200 µL of a 20% (v/v) dye concentrate diluted in demineralized water and filtered through Whatman No. 1 filter paper was added. After incubating for 10 min at room temperature, the absorbance was measured at 595 nm with a microplate spectrophotometer (µQuant MQX 200, BioTek Instruments, Winooski, VT, USA). All solutions were analyzed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!