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Ab76024

Manufactured by Abcam
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Sourced in United States

Ab76024 is a lab equipment product offered by Abcam. It serves as a core function with a focus on providing a factual and unbiased description without extrapolation on its intended use.

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Lab products found in correlation

Ab109235, by Cell Signaling Technology (2 mentions) Vinculin, by Merck Group (2 mentions) Cell lysis buffer, by Cell Signaling Technology (2 mentions) Laemmli sample buffer, by Bio-Rad (2 mentions) Criteriontris glycine extended gels, by Bio-Rad (2 mentions) Antibodygoat anti rabbit or goat anti mouse igg hrp, by Thermo Fisher Scientific (2 mentions) Azure c600, by Azure Biosystems (2 mentions) Image studio lite software version 5, by LI COR (2 mentions) Trans blot turbo system, by Bio-Rad (2 mentions) Ab229914, by Abcam (1 mentions) Ab186735, by Abcam (1 mentions) Ab32516, by Abcam (1 mentions) Ab81292, by Abcam (1 mentions) Ab26350, by Abcam (1 mentions) Mntbap, by Merck Group (1 mentions) Ab125066, by Abcam (1 mentions) β actin 4970, by Cell Signaling Technology (1 mentions) Nd1 19703 1 ap, by Proteintech (1 mentions)

3 protocols using ab76024

1

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
1*106 cells were lysed (cell lysis buffer, Cell signaling
technology) and denatured in 1x Laemmli sample buffer (Bio-rad) containing
2-mercaptoethanol (Sigma Aldrich). Proteins were separated on Criterion
Tris-Glycine eXtended gels (Bio-rad), transferred to PVDF membranes using the
Trans-Blot Turbo system (Bio-rad), incubated with primary antibodies targeting
Catalase (Cell Signaling #14097 & Abcam #ab76024), xanthine oxidase
(Abcam #ab109235), Bcl-2 (Cell Signaling #2870), and with secondary antibody
Goat Anti-Rabbit or Goat Anti-Mouse IgG-HRP (Thermo Fischer). Proteins were
visualized using chemiluminescence on an Azure C600 (Azure Biosystems).
Quantification was performed using LI-COR Image Studio Lite software version
5.2. Vinculin (Sigma Aldrich), tubulin or beta actin (Sigma Aldrich) was used to
normalize for protein input.
Kampen K.R., Sulima S.O., Verbelen B., Girardi T., Vereecke S., Rinaldi G., Verbeeck J., de Beeck J.O., Uyttebroeck A., Meijerink J.P., Moorman A.V., Harrison C.J., Spincemaille P., Cools J., Cassiman D., Fendt S.M., Vermeersch P, & De Keersmaecker K. (2018). The ribosomal RPL10 R98S mutation drives IRES-dependent BCL-2 translation in T-ALL. Leukemia, 33(2), 319-332.
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3

Deoxypodophyllotoxin Proteomic Analysis

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Deoxypodophyllotoxin (DPT) was obtained from Yuanye Bio-technology company (Shanghai, China). It was dissolved in DMSO and then stored at the concentration of 10 mmol/L. MnTBAP was from Sigma (St. Louis, MO, USA) and 3-Aminobenzamide (3AB), NAD+ and FK866 were all from MedChemExpress Company (Shanghai, China). The antibodies against TAX1BP1 (bs-13671R), NDUFS2 (bs-10455R) and NDUFS4 (bs-3961R) were all obtained from Bioss Antibodies (Beijing, China), against PARP1 (13371-1-AP), ND2 (19704-1-AP) and ND1 (19703-1-AP) were all from Proteintech Company (LA, USA), and against AIF (ab32516), GPX4 (ab125066), catalase (ab76024), H2AX (ab229914), TOMM20 (ab186735), phospho-H2AX at Ser139 (ab26350) and phospho-ATM at Ser1981 (ab81292) were all from Abcam company (Cambridge, UK), against PAR (#83732) and β-Actin (#4970) were both from Cell Signaling Technology Company (Beverly, MA, USA). Second antibody against rabbit (A0208) and against mouse (A0216) were from Beyotime Biotechnology (Nanjing, China).
Wang X.Z., Liang S.P., Chen X., Wang Z.C., Li C., Feng C.S., Lu S., He C., Wang Y.B., Chi G.F, & Ge P.F. (2023). TAX1BP1 contributes to deoxypodophyllotoxin-induced glioma cell parthanatos via inducing nuclear translocation of AIF by activation of mitochondrial respiratory chain complex I. Acta Pharmacologica Sinica, 44(9), 1906-1919.
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