Intestinal epithelial cell lines, including SW480, HT29, HCT116, HIEC, Caco2, and MC-38, were purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China). These cells were cultured in 10 cm plates with DMEM supplemented with 100 U/mL penicillin/streptomycin and 10% FBS. For in vitro treatment with rmIL-17A or rmIL-22 (R&D Systems), cells were starved in serum-free medium for 24 h and then switched to culture medium supplemented with 5% FBS in the presence or absence of rmIL-17A (100 ng/mL) or rmIL-22 (100 ng/mL) for 12 h. The pH of the medium was adjusted with HCl or NaOH.
Rmil 17a
Manufactured by R&D Systems
Sourced in United States
RmIL-17A is a recombinant protein that serves as a research tool. It is the murine (mouse) counterpart of the human interleukin-17A (IL-17A) cytokine. IL-17A plays a role in inflammatory and autoimmune responses.
Automatically generated - may contain errors
Lab products found in correlation
Rmil 22, by R&D Systems (2 mentions)
Caco 2, by National Collection of Authenticated Cell Cultures (1 mentions)
Hct116, by National Collection of Authenticated Cell Cultures (1 mentions)
Sw480, by National Collection of Authenticated Cell Cultures (1 mentions)
Two well culture insert, by Ibidi (1 mentions)
421 ml 025 cf, by R&D Systems (1 mentions)
Rmil 23, by BioLegend (1 mentions)
Sp600125, by Avantor (1 mentions)
Aldara cream, by 3M (1 mentions)
5 protocols using rmil 17a
1
In vitro Cytokine Treatment of Intestinal Cell Lines
2
IL-17A Induced Wound Healing Assay
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Control shGFP and shIL‐17RA CT26 cells were seeded at 3 × 104 cells per well in two‐well culture inserts (Ibidi) for attachment overnight. The inserts were removed at time 0 and the cells were stimulated with 100 ng/mL recombinant murine IL‐17A (rmIL‐17A; R&D Systems). The channel formed by the insert was imaged at 0, 6, 9, 12, and 24 h. At each time point, the MRI wound‐healing tool in ImageJ was used to measure the channel area that lacked cells.
3
Oxygen Glucose Deprivation Injury Neuronal Protection
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Recombinant mouse IL-17A (rmIL-17A, 421-ML-025/CF, R&D System) was respectively dissolved in the medium at final concentrations of 0.5, 1.0, 10,100, 250, and 500 ng/ml. When the neurons were treated with 1-h OGD/R 24 h, they were cultured in glucose-free DMEM with different concentrations of rmIL-17A and hypoxic condition (5% CO2 + 2% O2 + 93% N2) for 1 h, and then the neurobasal medium containing 2% B27 with different dose of rmIL-17A was recovered to supply with glucose and oxygen (5% CO2 + 95% O2) for 24 h. Finally, the neurons were collected and used for the next experiments.
4
Imiquimod-Induced Murine Psoriasis Model
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Eight- to 12-week-old female mice were shaved on the abdomen with an electrical shaver and depilated with Veet hair remover (10 (link)). Mice were topically treated with a 62.5 mg/day of cream containing 5% imiquimod (Aldara cream®, 3M pharmaceuticals) over eight consecutive days (11 (link)). Mice were sacrificed 4 h after the last application, and skin samples were collected for histology, gene expression or tissue processing. Carrier-free rmIL17A (R&D Systems, #7956ML025/CF) 500 ng/ear/day were injected daily for 8 days, then mice were sacrificed and ears were sampled for histology. Carrier-free rmIL23 (BioLegend, #589006) 1μg/ear/day or carrier-free rmAREG (PeproTech, #31536) 1μg/ear/day were injected daily for 4 days, then mice were sacrificed and ears were sampled for histology. When indicated, SP600125 30 mg/kg/d (VWR), AG1478 15mg/kg/d (VWR) or DMSO was injected intraperitoneally to mice, once per day for 9 days.
5
Evaluating IL-17A and IL-22 Effects on IECs
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Gpr65ΔIEC mice and Gpr65fl/fl littermates were intraperitoneally injected with rmIL-17A (1 μg/mouse, R&D Systems; Minneapolis, MN, USA) daily or rmIL-22 (2 μg/mouse, R&D Systems) every other day for 10 days. The other two groups of mice were administered PBS as a control. Mice were sacrificed on day 10, and IECs were isolated for further experimental assessments.
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