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Berberine chloride hydrate bbr

Manufactured by Merck Group
Sourced in United States

Berberine chloride hydrate (BBR) is a compound that is commercially available as a laboratory reagent. It is a naturally occurring isoquinoline alkaloid that can be isolated from various plant species. BBR serves as a chemical standard and is commonly used in research applications, though its specific functions and intended uses are not provided in this factual description.

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3 protocols using berberine chloride hydrate bbr

1

Berberine Chloride Hydrate Experiment Protocol

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Berberine chloride hydrate (BBR) was purchased from Sigma (St. Louis, MO, USA, Cat #14050). Common laboratory chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA). All antibodies used in this study are listed in Additional file 2: Table S1.
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2

Chitosan-based Nanomedicine Fabrication

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Chitosan (CS) flake (degree of deacetylation: %DD = 90 and Mw~300 kDa) was acquired from Seafresh Chitosan (Lab) Co., Ltd. (Bangkok, Thailand). Deoxycholic acid (DC) was purchased from Nacalai Tesque (Kyoto, Japan). Poly(ethylene glycol) monomethacrylate (PEGMA, MW = 486 g/mol) was obtained from Polysciences, Inc. (Warrington, PA, USA). 1-Ethyl-3-(3′-dimethylaminopropyl) carbodiimide (EDC) was purchased from TCI (Kawaguchi, Japan). N-hydroxysuccinimide (NHS) was purchased from Tokyo Kasei Kogyo Co., Ltd. (Kawaguchi, Japan). Paclitaxel (PTX, MW = 853.90 g/mol) was purchased from Calbiochem (Darmstadt, Germany). Berberine chloride hydrate (BBR, MW = 336.36 g/mol) was obtained from Sigma-Aldrich (St Louis, MO, USA). Acetic acid (CH3COOH) and ethanol (CH2OH) (AR grade) were purchased from Labscan, Co., Ltd. (Bangkok, Thailand). Dialysis membranes (MWCO = 1000 Da, 6000–8000 Da, and 12,000–14,000 Da) were purchased from Membrane Filtration Products, Inc. (Seguin, TX, USA). All chemicals were used without further purification.
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3

Berberine Chloride Hydrate Protocol

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Berberine chloride hydrate (BBR) was purchased from Sigma (St. Louis, MO, USA, Cat #14050). Oil Red O and common laboratory chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA). All antibodies used in this study are listed in Table S6.
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