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2 protocols using disodium hydrogen phosphate

1

Analytical Reagent Procurement for Chemical Analysis

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Analytical grade methanol, acetonitrile, and water were purchased from Fisher Scientific (Pittsburgh, PA, USA). Reagent grade chemicals, including methoxyamine hydrochloride, pyridine, and N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA), were obtained from Sigma Chemical Co. (St. Louis, MO, USA).
Analytical-grade chemicals, namely, acetic acid, 2,2-azinobis(3-ethylbenzothiazoline- 6-sulfonic acid) diammonium salt (ABTS), Folin–Ciocalteu’s phenol, formaldehyde solution, formic acid, methoxyamine hydrochloride, p-nitrophenol, p-nitrophenol β-d-glucopyranoside (p-NPG), potassium persulfate, pyridine, sodium hydroxide, sodium acetate, starch, N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), and tyrosine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, sodium dihydrogen phosphate, and disodium hydrogen phosphate were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan).
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2

Potato Dextrose Agar Media Preparation

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Potato dextrose agar media (Difco) was purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). HPLC-grade water, acetonitrile, and methanol were obtained from Fisher Scientific Co., Ltd. (Pittsburgh, PA, United States). Analytical-grade sodium dihydrogen phosphate, sodium chloride, sodium hydroxide, sodium carbonate, disodium hydrogen phosphate, and diethylene glycol were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). All remaining analytical-grade reagents and standard compounds used in this study were obtained from Sigma-Aldrich (St. Louis, MO, United States).
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