Fly husbandry was conducted according to standard procedures. All crosses were performed at 25°C. The WT strain used was Oregon R. Transgenic lines for expression of UASp-GFP-Ote (WT and mutants) were generated by site-directed insertions of our pUAS-K10attB-based vectors on the third chromosome in the attP154 strain (BestGene). The expression of the UASp-GFP-Ote and UASp-RFP-BAF transgenes in embryos for GFP affinity purification and for video microscopy was driven by matα4-GAL-VP16 (#7062; Bloomington Drosophila Stock Center). For genetic rescue experiments, the expression of UASp-GFP-Ote transgenes was driven by GAL4::VP16-nos.UTR (MVD1) (no. 4937; Bloomington Drosophila Stock Center). Otefin mutant alleles used were oteB279 (no. 16189; Bloomington Drosophila Stock Center) and oteDB (no. 5092; Bloomington Drosophila Stock Center). Fertility tests were done by placing single females with 2–3 Oregon R males in tubes containing grape juice agar and yeast paste at 25°C. Flies were transferred to new tubes every day and the number of eggs laid and percentages of hatched embryos were counted 24 h after removal of the flies from the tubes.
Attp154 strain
Manufactured by BestGene
The AttP154 strain is a laboratory tool used for genetic manipulation and research purposes. It serves as a host organism for specific plasmid or DNA integration. The core function of the AttP154 strain is to facilitate the incorporation of genetic material into the host's genome, enabling further experimentation and analysis.
Automatically generated - may contain errors
2 protocols using attp154 strain
1
Drosophila Husbandry and Genetic Rescue
2
Drosophila Husbandry and Genetic Rescue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fly husbandry was conducted according to standard procedures. All crosses were performed at 25°C. The WT strain used was Oregon R. Transgenic lines for expression of UASp-GFP-Ote (WT and mutants) were generated by site-directed insertions of our pUAS-K10attB-based vectors on the third chromosome in the attP154 strain (BestGene). The expression of the UASp-GFP-Ote and UASp-RFP-BAF transgenes in embryos for GFP affinity purification and for video microscopy was driven by matα4-GAL-VP16 (#7062; Bloomington Drosophila Stock Center). For genetic rescue experiments, the expression of UASp-GFP-Ote transgenes was driven by GAL4::VP16-nos.UTR (MVD1) (no. 4937; Bloomington Drosophila Stock Center). Otefin mutant alleles used were oteB279 (no. 16189; Bloomington Drosophila Stock Center) and oteDB (no. 5092; Bloomington Drosophila Stock Center). Fertility tests were done by placing single females with 2–3 Oregon R males in tubes containing grape juice agar and yeast paste at 25°C. Flies were transferred to new tubes every day and the number of eggs laid and percentages of hatched embryos were counted 24 h after removal of the flies from the tubes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!