Mouse anti human cxcr4 mab

Human glioma cell lines SHG-44 and U251 were purchased from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). NT21MP was designed by our laboratory and synthesized by GL Biochem Ltd. (Shanghai, China). The amino acid sequence information of the NT21MP is H-D-leu-D-Gly-D-Ala-D-Ser-D-Trp-D-His-D-Arg-D-Pro-D-Asp-D-Lys-Cys-Cys-Leu-Gly-Tyr-Gln-Lys-Arg-Pro-Leu-Pro-OH. Human-SDF-1α was purchased from PeproTech (Rocky Hill, NJ, USA). AMD3100 and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies against Bcl-2, Bax, caspase-3, cyclin D1 and β-actin were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A mouse anti-human CXCR4 mAb was purchased from Abcam (clone: 44716.111). Secondary antibodies conjugated to horseradish peroxidase (HRP) were purchased from ZSGB-Bio, Co., Ltd. (Beijing, China). Apoptosis kit was obtained from BD Biosciences (San Jose, CA, USA). Hoechst 33258 was purchased from Sigma-Aldrich. Reverse transcription kit was obtained from Thermo Fisher Scientific (Waltham, MA, USA) and the SYBR Premix Dimer Eraser™ reagent kit from Takara, Co., Ltd. (Shiga, Japan).