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Hiseq4000 v2

Manufactured by Illumina
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The HiSeq4000 v2 is a high-throughput DNA sequencing system designed for large-scale genomic projects. It utilizes Illumina's proprietary SBS (Sequencing by Synthesis) technology to generate high-quality sequencing data. The system is capable of producing up to 1.5 terabases of data per run, making it a powerful tool for researchers and clinicians working on applications such as whole-genome sequencing, transcriptome analysis, and metagenomic studies.

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Lab products found in correlation

Picogreen dsdna assay kit, by Roche (2 mentions) Qiaquick pcr purification kit, by Sage Science (2 mentions)

2 protocols using hiseq4000 v2

1

Illumina HiSeq4000 DNA Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted DNA was quantified with PicoGreen™ dsDNA Assay Kit, and 1 ng of input, or maximum 3.5 μl, gDNA was used in a 1:10 miniaturized Kapa HyperPlus protocol. Per sample libraries were quantified and pooled at equal nanomolar concentration. The pooled library was cleaned with the QIAquick PCR Purification Kit and size selected for fragments between 300 and 700 bp on the Sage Science PippinHT. The pooled library was sequenced as a paired-end 150-cycle run on an Illumina HiSeq4000 v2 at the UCSD IGM Genomics Center.
Mills R.H., Dulai P.S., Vázquez-Baeza Y., Sauceda C., Daniel N., Gerner R.R., Batachari L.E., Ochoa M.M., Zhu Q., Weldon K., Humphrey G., Carrillo-Terrazas M., Goldasich L.D., Bryant M., Raffatellu M., Quinn R.A., Gewirtz A.T., Chassaing B., Chu H., Sandborn W.J., Dorrestein P.C., Knight R, & Gonzalez D.J. (2022). Multi-omics analyses of the Ulcerative Colitis gut microbiome link Bacteroides vulgatus proteases with disease severity. Nature microbiology, 7(2), 262-276.
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2

Illumina HiSeq4000 DNA Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Extracted DNA was quantified with PicoGreen™ dsDNA Assay Kit, and 1 ng of input, or maximum 3.5 μl, gDNA was used in a 1:10 miniaturized Kapa HyperPlus protocol. Per sample libraries were quantified and pooled at equal nanomolar concentration. The pooled library was cleaned with the QIAquick PCR Purification Kit and size selected for fragments between 300 and 700 bp on the Sage Science PippinHT. The pooled library was sequenced as a paired-end 150-cycle run on an Illumina HiSeq4000 v2 at the UCSD IGM Genomics Center.
Mills R.H., Dulai P.S., Vázquez-Baeza Y., Sauceda C., Daniel N., Gerner R.R., Batachari L.E., Ochoa M.M., Zhu Q., Weldon K., Humphrey G., Carrillo-Terrazas M., Goldasich L.D., Bryant M., Raffatellu M., Quinn R.A., Gewirtz A.T., Chassaing B., Chu H., Sandborn W.J., Dorrestein P.C., Knight R, & Gonzalez D.J. (2022). Multi-omics analyses of the Ulcerative Colitis gut microbiome link Bacteroides vulgatus proteases with disease severity. Nature microbiology, 7(2), 262-276.
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