Interrupted 6 0 nylon sutures

Three horizontal 6-mm incisions were created on the shaved dorsum of anesthetized mice. A subcutaneous pocket was bluntly dissected in the fascial plane underlying the panniculus carnosus and either a 5 mm × 5 mm square of dHACM or a 5 mm × 5 mm square of control acellular fetal bovine dermal matrix of nonplacental origin (PriMatrix; TEI Biosciences, Inc, Boston, MA) was inserted. The third pocket did not receive an implant and acted as the sham surgical control. After surgery, the mice were placed on warming pads and allowed to fully recover from anesthesia before being returned to the institutional animal facility in separate cages. All incisions were closed using interrupted 6-0 nylon sutures (Ethicon Inc, Somerville, NJ). At days 3, 7, 14, and 28 after operation, mice were euthanized (n = 3 mice) and the three surgical sites, including implant and overlying skin, along with uninjured skin as a further negative control, were harvested for either fixation in 4% paraformaldehyde (12 h at 4°C) for histologic analysis or digested in 0.1% collagenase for flow cytometric analysis.

Experiments utilized a stented-wound healing model described previously (23 (link), 24 (link)). Animals were anesthetized, shaved, and prepared according to the standard sterile procedures. A six millimeter (6 mm) punch biopsy tool was used to create two circular, full-thickness cutaneous wounds (which extended through the panniculus carnosus) bilaterally on the shaved dorsal skin of diabetic mice. A donut-shaped silicone splint (Grace Bio-Labs, Bend, OR), with an external diameter of 12 mm and an internal diameter of 8 mm, was centred on the wound and affixed using cyanoacrylate adhesive (Elmer’s Inc., Columbus, OH) and interrupted 6-0 nylon sutures (Ethicon, Somerville, NJ). A semiocclusive dressing (Tegaderm, 3M, St. Paul, MN) was applied to cover the wound and splint. The animals were monitored daily.