The largest database of trusted experimental protocols

2 protocols using hoxa7

1

Immunohistochemistry and Immunoblotting Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cathepsin L (941) was from ImmunoChemistry Technologies (Bloomington, MN, USA). The following primary antibodies were used for immunohistochemistry, immunofluorescence, and immunoblotting (IB): V-ATPase G1 (16143–1-AP, Proteintech), V-ATPase V0A2 (SAB2100187, Sigma Aldrich), Vinculin (V9131, Sigma Aldrich), Ki67 (ab15580, Abcam), Podoplanin (D2–40; Ventana Medical Systems, Roche), MMP2 (10373–2-AP, Proteintech), POU3F2 (12,137, Cell Signaling), HOXA10 (TA590263, Origen), HOXA7 (ab211521, Abcam), V-ATPase G2 (25316–1-AP, Proteintech).
+ Open protocol
+ Expand
2

Multiparametric Analysis of Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysotracker, CellTrace Violet, FM lipophilic styryl (FM 1-43FX) and SYTO RNASelect dyes were from Life Technologies (Thermo Fisher Scientific). Acridine Orange was from ImmunoChemistry Technologies (Bloomington, MN, USA). PropidiumIodure (PI) and Annexin V were from BD Bioscience (FITC-Annexin V Apoptosis Detection Kit). Bafilomycin A1 (BafA1; sc-201550) was from Santa Cruz Biotechnologies whereas NH4Cl (254134) was from Sigma Aldrich.
The following primary antibodies were used for immunofluorescence, immunoblotting (IB) and flow cytometry (FACS) assays: V-ATPase G1 (16143-1-AP, Proteintech), Vinculin (V9131, Sigma Aldrich), Tsg101 (14497-1-AP, Proteintech), Ago2 (10686-1-AP, Proteintech), Clathrin (ab23440, Abcam), CD63 (sc-15363, Santa Cruz), CD9 (IB: 10626D, Thermo Fisher; FACS: 130-103-988, Miltenyi), CD81 (130-107-982, Miltenyi), Calnexin (ab31290, Abcam), Nestin (MAB 1259, R&D), Tuji (T3952, Sigma Aldrich), GFAP (G9269, Sigma Aldrich), CD11b (20991-1-AP, Proteintech), O4 (O7139, Sigma Aldrich), Olig2 (AV32753, Sigma Aldrich), CD31 (ab28364, Abcam), Vimentin (130-106-369, Miltenyi), POU3F2 (12137, Cell Signaling), HOXA10 (TA590263, Origen), HOXA7 (ab211521, Abcam), V-ATPase G2 (25316-1-AP, Proteintech), ARF6 (6ARF01, ThermoFisher Scientific), and GRP78 (3177, Cell Signaling).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!