Cultured HSCs derived from C57BL/6N-CD45.1 mice were transplanted with 1 × 106 whole BM cells obtained from C57BL/6N-CD45.1/ CD45.2 (F1) mice into lethally irradiated (9.5 Gy) C57BL/6N-CD45.2 mice. Peripheral blood (PB) was collected every 4 weeks after transplantation. PB mononuclear cells were stained with the combination of 7 antibodies; anti-mouse CD45.1-PE/Cy7 (Biolegend), anti-mouse CD45.2-BV421 (Biolegend), anti-mouse CD11b-PE (eBiosciences), anti-mouse Ly-6G/Ly-6C-PE (eBiosciences), anti-mouse CD45RA-APC/ eFluor780 (eBiosciences), anti-mouse CD4-APC (eBiosciences), and anti-mouse CD8-APC (eBiosciences). Cells were analyzed by FACS Aria Special Order Research Product (SORP Aria). For secondary BM transplantation, total BM cells were obtained from the primary mice and 1 × 106 cells were transferred into lethally irradiated C57BL/6N-CD45.2 mice. The chimerism was analyzed as above.
Anti mouse cd8 apc
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-mouse CD8-APC is a monoclonal antibody conjugated with Allophycocyanin (APC) that binds to the CD8 antigen expressed on the surface of mouse T cells. It is used for the identification and analysis of CD8+ T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cd4 apc, by Thermo Fisher Scientific (2 mentions)
Anti mouse cd11b pe, by Thermo Fisher Scientific (2 mentions)
Anti mouse cd45.1 pe cy7, by BioLegend (2 mentions)
Anti mouse cd45.2 bv421, by BioLegend (2 mentions)
Anti mouse ly 6g ly 6c pe, by Thermo Fisher Scientific (2 mentions)
Anti mouse cd45ra apc efluor780, by Thermo Fisher Scientific (2 mentions)
3 protocols using anti mouse cd8 apc
1
Tracking Hematopoietic Stem Cell Engraftment
2
Tracking Hematopoietic Stem Cell Engraftment
3
Biochemical and Immunological Assays in Lupus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Urine protein quantitation kit (Lot: 20150407), blood urea nitrogen (BUN) determination kit (Lot: 20150401), and serum creatinine (SCr) determination kit (Lot: 20150402) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ELISA kit for mouse anti-dsDNA autoantibody (Lot: 1503251), ELISA kit for mouse CH50 (Lot: 1408212), ELISA kit for mouse CRP (Lot: 1408251), and ELISA kit for mouse IgE (Lot: 1408081) were provided by Shanghai Westang Bio-Tech Co., Ltd. (Shanghai, China). Anti-mouse CD3 FITC (Clone: 145-2c11, Lot: E00058-1631), anti-mouse CD8 APC (Clone: 53-6.7, Lot: E07055-1634), and anti-mouse CD54 PE (Clone: YN1/1.7.4, Lot: E01295-1633) were purchased from eBioscience Inc. (San Diego, CA, USA). Antibodies against p65 NF-κB, Sirt1, and Nrf2 were provided by Santa Cruz Biotechnology (Santa Cruz, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!