The isolated cells were characterized using flow cytometry. The expression of the surface markers CD44 (Cat No. AM310‐10 M, BioGenex), CD45 (Cat No. AM111‐10 M, BioGenex), CD90 (Cat No. 21270906, ImmunoTools), CD105 (Cat no. 21271054, ImmunoTools) and CD73 (Cat no. 21270733, ImmunoTools) was investigated.
Cd105
Manufactured by Immunotools
Sourced in Germany
CD105 is a glycoprotein that functions as an accessory receptor for transforming growth factor beta (TGF-β) signaling. It plays a role in angiogenesis and endothelial cell proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Lsr 2 3 lasers cytofluorometer, by BD (1 mentions)
Diva software, by BD (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Insulin transferrin selenium, by Merck Group (1 mentions)
3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Indomethacin, by Merck Group (1 mentions)
β glycerophosphate, by Merck Group (1 mentions)
Ascorbate 2 phosphate, by Merck Group (1 mentions)
4 protocols using cd105
1
Mesenchymal Stem Cell Characterization
2
Multicolor flow cytometry analysis
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FITC, PE, or APC conjugated mouse anti-human CD73, CD90, CD105, CD3, CD41 CD62P, human leukocyte antigen (HLA)-DR, intercellular adhesion molecule 1 (ICAM-1), CD8 (all from Immunotools) were used to label the various cells tested. Analyses were performed using the “LSR II 3 lasers” cytofluorometer and the Diva software (both were from BD Biosciences).
3
Mesenchymal Stem Cell Surface Marker Profiling
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Flow cytometry was used to examine the surface protein expression of mesenchymal stem cell markers44 (link),45 (link). The cells were detached using trypsin/EDTA solution to obtain a single cell suspension. The cells were immunostained with primary antibodies conjugated with fluorescent dye, comprising CD44 (Cat No. AM310‐10M, BioGenex, Fremont CA, USA), CD45 (Cat No. AM111‐10M, BioGenex), CD73 (Cat No. 21270733, ImmunoTools, Friesoythe, Germany), CD90 (Cat No. 21270906, ImmunoTools), and CD105 (Cat No. 21271054, ImmunoTools). Flow cytometry analysis was performed using a FACSCalibur Flow cytometer (BD Biosciences, NJ, USA).
4
Aloe vera-based Adipogenic Differentiation
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Aloe barbadensis (Aloe vera) from the botanical garden, we bought gelatin from bovine skin from Sigma (USA), (Type B, powder, Bioreagent, appropriate for cell culture), isopropyl alcohol (IPA: C3H8O), ethanol (Ethyl Alcohol: C2H6O) from the Hayman, Dulbecco's Modified Eagle's medium-F12 (DMEM-F12), 1X Phosphate Buffer Solution (PBS), carbinol, chloroform, FBS (fetal bovine serum) GIBCO, trypsin, dexamethasone (Sigma), 3-isobutyl-1-methyl-xanthine (IBMX, Sigma), insulin-transferrin-selenium (ITS), indomethacin (Sigma), ascorbate-2 phosphate, β-glycero phosphate (Sigma), and MTT [3-(4, 5-dimethyl-thiazol)-2, 5-di-phenyl tetrazolium bromide] was purchased from Invitrogen. 4% paraformaldehyde (R&D Systems), F-actin conjugated phalloidin (red) (Invitrogen), DAPI (Sigma), CD34, CD45, CD73, CD90, CD105, and IgG anti-mouse-PE-tagged secondary antibody from the Immuno Tools, Friesoythe, Germany.
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