Anti mir 9

Manufactured by Thermo Fisher Scientific

Sourced in China

anti-miR-9 is a synthetic, single-stranded RNA molecule designed to inhibit the activity of microRNA-9 (miR-9). MicroRNAs are small, non-coding RNA molecules that play a role in regulating gene expression. The core function of anti-miR-9 is to bind to and inactivate miR-9, thereby modulating the expression of genes influenced by miR-9.

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Lab products found in correlation

Siport neofx transfection agent, by Thermo Fisher Scientific (1 mentions) Pre mir 335, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000 reagent, by Thermo Fisher Scientific (1 mentions)

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Anti-miRs (5 citations)

2 protocols using anti mir 9

miR-9 Inhibitor Transfection in ES-2 Cells

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Both miR-9 inhibitor (anti-miR-9) and the scrambled miR-9 inhibitor (negative
control) were purchased from Applied Biosystems (China) and used according to the
manufacturer’s instruction. ES-2 cells (3×10⁵) were transiently
transfected with anti-miR-9 (100 nM) or negative control (100 nM) with siPORT™ NeoFX™
Transfection Agent (Applied Biosystems; 10 µL in 200 µL of OPTI-MEM¯ I medium without
serum) for 48 h. Cells were then harvested and analyzed.

Huang X., Teng Y., Yang H, & Ma J. (2016). Propofol inhibits invasion and growth of ovarian cancer cells via regulating miR-9/NF-κB signal. Brazilian Journal of Medical and Biological Research, 49(12), e5717.

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Modulating miRNA expression in cells

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Cells were seeded at 250,000 cells/well in 6 well-plate or at 1,500,000 cells/100mm dish. After 24 h, cells were transfected with one of the RNA oligonucleotides (Anti-miR-9, anti-miR-335, anti-miR-338-3p, Negative Control (NC)-anti-miR, pre-miR-338-3p, pre-miR-335, pre-miR-335* or Negative Control (NC)-pre-miR; Applied Biosystems) at the 50 nM final concentration, using Lipofectamine 2000 reagent (Invitrogen) and Opti-MEM medium according to the manufacturer’s instructions. After 6 h of transfection, the medium was changed to fresh growth medium. Cells were collected after 48 h of transfection.

Meseguer S., Boix O., Navarro-González C., Villarroya M., Boutoual R., Emperador S., García-Arumí E., Montoya J, & Armengod M.E. (2017). microRNA-mediated differential expression of TRMU, GTPBP3 and MTO1 in cell models of mitochondrial-DNA diseases. Scientific Reports, 7, 6209.

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