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Ricolinostat

Manufactured by Selleck Chemicals
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Sourced in United States, Morocco

Ricolinostat is a chemical compound used in research applications as a pharmaceutical ingredient. It functions as a selective inhibitor of histone deacetylase 6 (HDAC6), an enzyme involved in the regulation of cellular processes. The core function of Ricolinostat is to modulate the activity of HDAC6 for experimental purposes.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Tubacin, by Selleck Chemicals (2 mentions) Vorinostat, by Cayman Chemical (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Panobinostat, by LC Laboratories (2 mentions) Bortezomib, by LC Laboratories (2 mentions) Penicillin streptomycin, by Lonza (2 mentions) Rpmi8226, by American Type Culture Collection (2 mentions) Su dhl 4, by American Type Culture Collection (2 mentions) Daudi, by American Type Culture Collection (2 mentions) Panobinostat, by Selleck Chemicals (2 mentions) Entinostat, by Selleck Chemicals (2 mentions) Romidepsin, by Selleck Chemicals (2 mentions) Peg300, by Merck Group (1 mentions) Carfilzomib, by Selleck Chemicals (1 mentions) Vorinostat, by Selleck Chemicals (1 mentions) Tazmetostat, by Selleck Chemicals (1 mentions) Ml324, by Selleck Chemicals (1 mentions) Otx015, by Selleck Chemicals (1 mentions)

11 protocols using ricolinostat

1

Generating Bortezomib-Refractory Myeloma and Lymphoma Cell Lines

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Multiple myeloma and lymphoma cell lines RPMI-8226, Kas6, Daudi, and SUDHL4, were from American Type Culture Collection (ATCC, Manassas, VA). To generate bortezomib-refractory cells, parental RPMI-8226 or Kas6 cells (designated as RMPI-8226wt or Kas6wt) were chronically exposed to increasing concentrations of bortezomib to generate resistant lines (designated as RMPI-8226v10r or Kas6v10r)[29 (link),30 (link)]. Resistant lines were then cultured in the presence of 10 nM bortezomib. Cell line identities were confirmed by Short Tandem Repeat DNA profiling as conducted by the MD Anderson Cancer Center Characterized Cell Line Core. All cell lines were cultured in RPMI1640 (Thermo Fisher Scientific, Waltham, MA) with 10% FBS (Thermo Fisher Scientific, Waltham, MA), 1% L-glutamine (Thermo Fisher Scientific, Waltham, MA), and 1% penicillin/streptomycin (Lonza, Basel, Switzerland) at 37 °C in a 5% CO2 incubator. All cultures were free of bacterial, fungal, and mycoplasma contamination (mycoplasma contamination was tested every 6 months). The following drugs were used for the study: bortezomib, LC Laboratories, (Woburn, MA); vorinostat, Cayman Chemical, (Ann Arbor, MI); panobinostat, LC Laboratories, (Woburn, MA); ricolinostat, Selleck Chemicals, (Houston, TX); tubacin, Selleck Chemicals, (Houston, TX).
Cheng T., Kiser K., Grasse L., Iles L., Bartholomeusz G., Samaniego F., Orlowski R.Z, & Chandra J. (2021). Expression of histone deacetylase (HDAC) family members in bortezomib-refractory multiple myeloma and modulation by panobinostat. Cancer Drug Resistance, 4(4), 888-902.
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2

Combination Therapy for Cancer Treatment

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Entinostat (MS275) and anti-CSF1R mIgG2 antibody (SNDX-ms6352) were obtained from Syndax Pharmaceuticals, Inc. Ricolinostat (ACY-1215) was purchased from Selleck Chemicals. For the in vivo studies, entinostat was dissolved in DMSO, and then diluted with ddH2O. Ricolinostat was dissolved in DMSO, and then diluted with PEG300 (Sigma Aldrich) and ddH2O to achieve 2% DMSO/30%DMSO in H2O. Entinostat was orally administered to the mice at 10 mg/kg every day, and 50 mg/kg of Ricolinostat was administered daily i.p. Anti-CSF1R antibody was diluted with PBS and injected i.p. to the mice (30 mg/kg) twice a week.
Hashimoto A., Fukumoto T., Zhang R, & Gabrilovich D. (2020). Selective targeting of different populations of myeloid-derived suppressor cells by histone deacetylase inhibitors. Cancer immunology, immunotherapy : CII, 69(9), 1929-1936.
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3

Preparation of HDACi for NMVM

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Panobinostat (LBH589), entinostat (MS275) and ricolinostat (ACY-1215) were purchased from Selleck Chemicals (Houston, TX, USA), dissolved in DMSO, and stored at −20 °C. The 100-mM DMSO solutions of LBH589, MS-275 and ACY-1215 were diluted to the desired experimental test concentrations in M199 and applied to NMVM overnight for 18–24 h before experimental procedures. Final DMSO levels were <0.005% (vol/vol).
Zhang X., Patel D., Xu Q, & Veenstra R. (2018). Differences in Functional Expression of Connexin43 and NaV1.5 by Pan- and Class-Selective Histone Deacetylase Inhibition in Heart. International Journal of Molecular Sciences, 19(8), 2288.
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4

Multiple Myeloma Cell Lines and Compounds

Cited 2 times
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Cell lines (KMS11, OPM2, and ANBL6) were purchased from American Type Culture Collection (Manassas, VA, USA). MM.1S BzR cells were a gift from Dr. Brian Van Ness (U. of Minnesota) and have been described previously [25 (link)]. LTI6426 was provided by Leukogene Therapeutics, Inc. (Charleston, SC) with purity and identity that was confirmed by liquid chromatograph–mass spectrometry (LC–MS) and nuclear magnetic resonance (NMR) as shown previously [16 (link)]. Bortezomib (Catalog No. S1013), carfilzomib (Catalog No. S2853), panobinostat (Catalog No. S1030), vorinostat (Catalog No. S1047), ricolinostat (Catalog No. S8001), entinostat (Catalog No. S1053), romidepsin (Catalog No. S3020), tazmetostat (Catalog No. S7128), ML324 (Catalog No. S7296), JQ1 (Catalog No. S7110), OTX015 (Catalog No. S7360), ABBV744 (Catalog No. S8723), IBET151 (Catalog No. S2780), and GSK-LSD1 (Catalog No. S7574) were purchased from Selleck Chemicals (Houston, TX, USA).
Robinson R.M., Basar A.P., Reyes L., Duncan R.M., Li H, & Dolloff N.G. (2022). PDI inhibitor LTI6426 enhances panobinostat efficacy in preclinical models of multiple myeloma. Cancer Chemotherapy and Pharmacology, 89(5), 643-653.
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5

Evaluation of Crizotinib, Ricolinostat, and Chidamide

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Crizotinib (CAS No. 877399-52-5) and ricolinostat (CAS No. 1316214-52-4) were purchased from Selleck Chemicals (Houston, TX, USA). Chidamide (CAS No. 743420-02-2) was kindly provided by Shenzhen Chipscreen Biosciences Ltd. (Shenzhen, China). Human hepatocyte growth factor (HGF; Entrez Gene 3082) was purchased from Sino Biological, Inc. (10463-HNAS, Beijing, China).
Ding N., You A., Tian W., Gu L, & Deng D. (2020). Chidamide increases the sensitivity of Non-small Cell Lung Cancer to Crizotinib by decreasing c-MET mRNA methylation. International Journal of Biological Sciences, 16(14), 2595-2611.
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6

Apoptosis and Cytotoxicity Assays

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Fetal calf serum (FCS), phosphate-buffered saline (PBS), Dulbecco’s Modified Eagle’s medium (DMEM), RPMI medium, trypsin-EDTA, penicillin and streptomycin were purchased from Gibco, Invitrogen (CA, USA). Ricolinostat (ACY-1215) was purchased from Selleckchem (Houston, TX, USA). Annexin V: FITC Apoptosis Detection Kit was purchased from BD Pharmingen (San Diego, CA, USA). Tools Cell Counting (CCk-8) Kit was purchased from Biotools Co., Ltd (Taipei, Taiwan). MTT dye, doxorubicin, mitoxantrone and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA), unless stated otherwise.
Wu C.P., Hsieh Y.J., Murakami M., Vahedi S., Hsiao S.H., Yeh N., Chou A.W., Li Y.Q., Wu Y.S., Yu J.S, & Ambudkar S.V. (2018). Human ATP-binding cassette transporters ABCB1 and ABCG2 confer resistance to histone deacetylase 6 inhibitor ricolinostat (ACY-1215) in cancer cell lines. Biochemical pharmacology, 155, 316-325.
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7

Investigating miR-30d Inhibitor Effects on Human Cell Lines

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The human cell lines EC109, KYSE150, TE-1, TE-13, and HUVEC were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in DMEM medium (Gibco, USA) containing 10% fetal bovine serum. Ricolinostat (ACY-1215) was purchased from Selleck Chemicals (TX, USA) and was dissolved in DMSO to obtain a stock concentration of 100 mM. MiR-30d inhibitor was purchased from RiboBio Company (Guangzhou, China) and was dissolved in RNase-free water to obtain a concentration of 20 µM.
Cao J., Lv W., Wang L., Xu J., Yuan P., Huang S., He Z, & Hu J. (2018). Ricolinostat (ACY-1215) suppresses proliferation and promotes apoptosis in esophageal squamous cell carcinoma via miR-30d/PI3K/AKT/mTOR and ERK pathways. Cell Death & Disease, 9(8), 817.
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8

Generating Bortezomib-Refractory Myeloma and Lymphoma Cell Lines

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Multiple myeloma and lymphoma cell lines RPMI-8226, Kas6, Daudi, and SUDHL4, were from American Type Culture Collection (ATCC, Manassas, VA). To generate bortezomib-refractory cells, parental RPMI-8226 or Kas6 cells (designated as RMPI-8226wt or Kas6wt) were chronically exposed to increasing concentrations of bortezomib to generate resistant lines (designated as RMPI-8226v10r or Kas6v10r)[29 (link),30 (link)]. Resistant lines were then cultured in the presence of 10 nM bortezomib. Cell line identities were confirmed by Short Tandem Repeat DNA profiling as conducted by the MD Anderson Cancer Center Characterized Cell Line Core. All cell lines were cultured in RPMI1640 (Thermo Fisher Scientific, Waltham, MA) with 10% FBS (Thermo Fisher Scientific, Waltham, MA), 1% L-glutamine (Thermo Fisher Scientific, Waltham, MA), and 1% penicillin/streptomycin (Lonza, Basel, Switzerland) at 37 °C in a 5% CO2 incubator. All cultures were free of bacterial, fungal, and mycoplasma contamination (mycoplasma contamination was tested every 6 months). The following drugs were used for the study: bortezomib, LC Laboratories, (Woburn, MA); vorinostat, Cayman Chemical, (Ann Arbor, MI); panobinostat, LC Laboratories, (Woburn, MA); ricolinostat, Selleck Chemicals, (Houston, TX); tubacin, Selleck Chemicals, (Houston, TX).
Cheng T., Kiser K., Grasse L., Iles L., Bartholomeusz G., Samaniego F., Orlowski R.Z, & Chandra J. (2021). Expression of histone deacetylase (HDAC) family members in bortezomib-refractory multiple myeloma and modulation by panobinostat. Cancer Drug Resistance, 4(4), 888-902.
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9

Cell Line Culturing and Chemical Inhibitor Preparation

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The HH, H9 and HUT78 cell lines were a generous gift from Dr Giandomenico Russo (Istituto Dermopatico dell'Immacolata, IDI–IRCCS, Rome, Italy). The SeAX cell line was a generous gift from Dr Katarzyna Izykowska (Institute of Human Genetics, Polish Academy of Science, Poznan, Poland). 293T cells were purchased from ATCC. The cells were maintained in RPMI-1640 medium (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum, L-glutamine (2 mM), penicillin (100 U/ml) and streptomycin (100 µg/ml). HDAC inhibitors (ricolinostat, citarinostat and resminostat), as well as PI3K inhibitors (duvelisib, copanlisib and pictilisib) were purchased from Selleckchem. Inhibitors were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; Merck KGaA) or 10% trifluoric acid (Sigma-Aldrich; Merck KGaA) in the case of copanlisib.
Bobrowicz M., Slusarczyk A., Domagala J., Dwojak M., Ignatova D., Chang Y.T., Iselin C., Miazek-Zapala N., Marhelava K., Guenova E, & Winiarska M. (2020). Selective inhibition of HDAC6 sensitizes cutaneous T-cell lymphoma to PI3K inhibitors. Oncology Letters, 20(1), 533-540.
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10

Pharmacological Evaluation of HDAC Inhibitors

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Panobinostat (LBH589), romidepsin, ACY-1215 (ricolinostat), and CUDC-907 were obtained from Selleck Chemicals (Houston, Texas, USA). Each chemical was dissolved in dimethyl sulfoxide (DMSO) and added to the culture medium at the indicated concentrations for in vitro studies. For in vivo experimentation, CUDC-907 was dissolved in DMSO and PEG300, after which Tween80 (polyoxyethylene sorbitan monooleate) and sterile water were added (CUDC-907 5%, PEG300 40%, Tween80 5%, water 50%). Daratumumab for in vitro studies was kindly provided by Janssen Pharmaceutical K. K (Beerse, Antwerpen, Belgium). Elotuzumab was obtained from Bristol-Myers Squibb (New York, New York, USA). Daratumumab and Elotuzumab were dissolved in sterile water. CHIR-99021 was obtained from Cayman Chemical Company (Ann Arbor, Michigan, USA).
Hirano M., Imai Y., Kaito Y., Murayama T., Sato K., Ishida T., Yamamoto J., Ito T., Futami M., Ri M., Yasui H., Denda T., Tanaka Y., Ota Y., Nojima M., Kamikubo Y., Gotoh N., Iida S., Handa H, & Tojo A. (2021). Small-molecule HDAC and Akt inhibitors suppress tumor growth and enhance immunotherapy in multiple myeloma. Journal of Experimental & Clinical Cancer Research : CR, 40, 110.
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