Glyco tek affinity column

Manufactured by Helena Laboratories

Sourced in United States

The Glyco-Tek Affinity column is a laboratory instrument used for the purification and separation of glycoproteins and glycans. It employs affinity chromatography principles to selectively bind and elute target biomolecules based on their glycosylation patterns.

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Lab products found in correlation

Alphatrak glucometer, by Abbott (2 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Alphatrak, by Abbott (1 mentions)

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Glyco-tek affinity column kit

5 protocols using glyco tek affinity column

Comprehensive Metabolic Profiling of Animals

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For each animal, bodyweight was recorded and fasting blood glucose (FBG) levels were measured with an AlphaTrak Glucometer (Abbott Laboratories, Abbott Park, IL, USA) weekly. Glycated haemoglobin (GHb) levels were determined using a Glyco‐Tek Affinity column (catalog no. 5351; Helena Laboratories, Beaumont, TX, USA) at the Michigan Diabetes Research and Training Center Chemistry Core. Fasting plasma insulin, total cholesterol and total triglycerides were measured by the National Mouse Metabolic Phenotyping Center (Vanderbilt University, Nashville, TN, USA).

Hinder L.M., Park M., Rumora A.E., Hur J., Eichinger F., Pennathur S., Kretzler M., Brosius FC I.I.I, & Feldman E.L. (2017). Comparative RNA‐Seq transcriptome analyses reveal distinct metabolic pathways in diabetic nerve and kidney disease. Journal of Cellular and Molecular Medicine, 21(9), 2140-2152.

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Measuring Metabolic Biomarkers and Liver Steatosis

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Additional measurements taken included nonfasting blood glucose and hemoglobin A₁C levels (Glyco-tek affinity column; Helena Laboratories, Beaumont, TX, USA). Serum was collected for determining levels of free fatty acid, triglyceride, and free cholesterol using commercial kits from Roche Diagnostics; Sigma-Aldrich Corp.; and Bio Vision (Mountain View, CA, USA), respectively. Liver steatosis was determined as previously described.¹⁰ Acquired images were analyzed for % area fraction of lipid droplets using Image J software (http://imagej.nih.gov/ij/; provided in the public domain by the National Institutes of Health, Bethesda, MD, USA).

Yorek M.S., Davidson E.P., Poolman P., Coppey L.J., Obrosov A., Holmes A., Kardon R.H, & Yorek M.A. (2016). Corneal Sensitivity to Hyperosmolar Eye Drops: A Novel Behavioral Assay to Assess Diabetic Peripheral Neuropathy. Investigative Ophthalmology & Visual Science, 57(6), 2412-2419.

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Comprehensive Metabolic Profiling and Oxidative Stress Analysis

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Additional measurements were taken including nonfasting blood glucose and hemoglobin A₁C levels (Glyco-Tek affinity column, Helena Laboratories, Beaumont, TX). Serum was collected for determining levels of free fatty acid, triglyceride, free cholesterol, and leptin, using commercial kits from Roche Diagnostics, Mannheim, Germany; Sigma Chemical Co., St. Louis, MO; Bio Vision, Mountain View, CA; and ALPCO diagnostics, respectively. Liver segments were collected for analysis of triglyceride deposition. Liver samples were embedded in Tissue-Tek O.C.T. compound (Sakura Finetek, Torrance, CA), sectioned (10 μm thickness), and stained with oil red O to determine area of triglyceride deposition in each group by NIH Image [23 ]. Segments of sciatic nerve were used to determine nitrotyrosine staining as a marker of oxidative stress by Western blot analysis as previously described [24 (link)].

Davidson E.P., Coppey L.J., Holmes A., Lupachyk S., Dake B.L., Oltman C.L., Peterson R.G, & Yorek M.A. (2014). Characterization of Diabetic Neuropathy in the Zucker Diabetic Sprague-Dawley Rat: A New Animal Model for Type 2 Diabetes. Journal of Diabetes Research, 2014, 714273.

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Metabolic Profiling of Diabetic Mice

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Diabetic phenotype was established with weekly body weights, and by measuring fasting blood glucose levels (4 h) and plasma metabolite concentrations. Plasma analytes were measured at multiple time points depending on the experiment and are detailed in Figs S1 and S4; they included plasma insulin, glycated hemoglobin (GHb), total cholesterol and total triglycerides. Fasting blood glucose levels were measured using an AlphaTrak glucometer (Abbott Laboratories, Abbott Park, IL, USA). Readings above the 750 mg dL⁻¹ range of the glucometer were arbitrarily set at 750 mg dL⁻¹ for statistical analysis. Plasma insulin, total triglyceride and total cholesterol concentrations were measured by the Mouse Metabolic Phenotyping Center (MMPC; Vanderbilt University, Nashville, TN, USA; University of Cincinnati, Cincinnati, OH, USA). Blood GHb concentrations were measured using a Glyco-Tek Affinity column (Helena Laboratories, Beaumont, TX, USA) by the Michigan Diabetes Research Center's Chemistry Core.

Elzinga S.E., Eid S.A., McGregor B.A., Jang D.G., Hinder L.M., Dauch J.R., Hayes J.M., Zhang H., Guo K., Pennathur S., Kretzler M., Brosius F.C., Koubek E.J., Feldman E.L, & Hur J. (2023). Transcriptomic analysis of diabetic kidney disease and neuropathy in mouse models of type 1 and type 2 diabetes. Disease Models & Mechanisms, 16(10), dmm050080.

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Phenotyping Diabetic Mouse Model

Cited 3 times

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Control db/+ and db/db mice were phenotyped for body weight, fasting blood glucose (FBG), and glycated hemoglobin (GHb) levels (n = 5–6 for each group per time point). FBG levels were measured with an AlphaTrak Glucometer (Abbott Laboratories, Abbott Park, IL) for 8 and 16 wk mice and a standard Glucometer (OneTouch; LifeScan Inc., Milpitas, CA) for 24 wk mice. GHb levels were determined using a Glyco-Tek Affinity column (Helena Laboratories, Beaumont, TX) at the Michigan Diabetes Research and Training Center Chemistry Core. Peripheral nerve function was assessed at 8, 16, and 24 wk according to Diabetic Complications Consortium guidelines (https://www.diacomp.org/shared/protocols.aspx). Motor (sciatic) and sensory (sural) nerve conduction velocities (NCVs), hind-paw withdrawal latency from a thermal stimulus, and intraepidermal nerve fiber density were measured for large and small fiber nerve function, and small fiber loss using our previously published protocols^{16 (link)–19}. Data from 16 wk and 24 wk cohorts are previously published^{9 (link), 11 (link)}.

Hinder L.M., Murdock B.J., Park M., Bender D.E., O’Brien P.D., Rumora A.E., Hur J, & Feldman E.L. (2018). Transcriptional networks of progressive diabetic peripheral neuropathy in the db/db mouse model of type 2 diabetes: an inflammatory story. Experimental neurology, 305, 33-43.

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