Pierce bicinchoninic acid protein bca assay

Manufactured by Beyotime

Sourced in China

The Pierce Bicinchoninic acid (BCA) Protein Assay is a colorimetric assay for the quantitative determination of total protein concentration. It relies on the reduction of Cu2+ to Cu+ by protein in an alkaline medium and the subsequent colorimetric detection of the Cu+ by bicinchoninic acid.

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Pierce bicinchoninic acid (BCA) protein assay kit Bicinchoninic acid (BCA) protein assay Bicinchoninic acid (BCA) assay BCA Bicinchoninic acid assay BCA protein assay Pierce BCA BCA assay Protein assay

2 protocols using pierce bicinchoninic acid protein bca assay

Encapsulated Enzyme Nanoparticle Characterization

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The supernatants obtained from the purification of the ES-NPs were collected to determine their loading and EE. The concentration of ES in the supernatant was measured using a Pierce Bicinchoninic acid Protein (BCA) assay (Beyotime Biotechnology, Shanghai, China). The results of the assay were validated using purified ES with a detection limit of 0.5–280 μg/mL. The amount of ES was calculated according to the standard curve (R²=0.9993). The loading efficiency (LE) and EE of the ES-NPs were expressed as follows:

LE % = \frac{(Total amount of ES added) - (Free amount of ES)}{ES - loaded NPs dry weight} \times 100 %

EE % = \frac{(Total amount of ES added) - (free amount of ES)}{Total amount of ES added} \times 100 %

Ding R.L., Xie F., Hu Y., Fu S.Z., Wu J.B., Fan J., He W.F., He Y., Yang L.L., Lin S, & Wen Q.L. (2017). Preparation of endostatin-loaded chitosan nanoparticles and evaluation of the antitumor effect of such nanoparticles on the Lewis lung cancer model. Drug Delivery, 24(1), 300-308.

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Determining Encapsulation Efficiency of Enzyme-Loaded Nanoparticles

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Supernatants obtained from the purification of the ES-NPs were collected to determine particle loading and EE. The concentration of ES in the supernatant was measured using a Pierce Bicinchoninic acid Protein (BCA) assay (Beyotime Biotechnology, China) (Mukherjee et al., 2008 ; Xu et al., 2014 (link)). The results of the assay were validated using purified ES with a detection limit of 0.5–280 mg/mL. The amount of ES was calculated according to the standard curve (R² = 0.9992). The DL and EE of the ES-NPs were expressed as follows:

D L % = \frac{T o t a l a m o u n t o f E S a d d e d - F r e e a m o u n t o f E S}{E S - l o a d e d N P s d r y w e i g h t} \times 100 %

E E % = \frac{T o t a l a m o u n t o f E S a d d e d - F r e e a m o u n t o f E S}{T o t a l a m o u n t o f E S a d d e d} \times 100 %

Xie F., Ding R.L., He W.F., Liu Z.J., Fu S.Z., Wu J.B., Yang L.L., Lin S, & Wen Q.L. (2017). In vivo antitumor effect of endostatin-loaded chitosan nanoparticles combined with paclitaxel on Lewis lung carcinoma. Drug Delivery, 24(1), 1410-1418.

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