Pe conjugated anti pd 1

The cells were analyzed on a MACSQuant Flow Cytometer (MiltenyiBiotec) using the following specific antibodies: VioletFluor^TM 450-conjugated anti-B220 (TONBO Biosciences), Brilliant Violet 510^TM-conjugated anti-CD4 (BioLegend), Brilliant Violet 510^TM-conjugated anti-CD8a (BioLegend), phycoerythrin (PE)-conjugated anti-CD138 (BioLegend), Alexa Fluor 647-conjugated anti-GL7 (BioLegend), PE-conjugated anti-CD62L (BD Biosciences), allophycocyanin (APC)-conjugated anti-CD44 (eBioscience), APC-conjugated anti-CD86 (TONBO Biosciences), PE-conjugated anti-CD69 (BioLegend), PE-conjugated anti-CD23 (BioLegend), Alexa Fluor 647-conjugated anti-CD21 (BD Pharmingen), PE-conjugated anti-PD-1 (TONBO Biosciences), Brilliant Violet 421^TM-conjugated anti-CXCR5 (BioLegend), Alexa Fluor 647-conjugated ICOS (BioLegend), eFour450-conjugated anti-CD127 (eBioscience), PE-conjugated anti-CD25 (TONBO Biosciences) and APC-conjugated anti-CTLA4 (TONBO Biosciences). Data analysis was conducted with FlowJo software (v7.6.5; FLOWJO, LLC).

A MACSQuant Flow Cytometer (Miltenyi Biotec) was used to analyze whether the cells utilized the following specific antibodies: Violet Fluor^™ 450-conjugated anti-B220 (Tonbo Biosciences); Alexa Fluor 647-conjugated anti-CD4 (BD Pharmingen); Brilliant Violet 510^™-conjugated anti-CD4 (BioLegend); Brilliant Violet 510^™-conjugated anti-CD8a (BioLegend); Alexa Fluor 647-conjugated anti-GL7 (BioLegend), phycoerythrin (PE)-conjugated anti-CD62L (BD Biosciences); allophycocyanin (APC)-conjugated anti-CD44 (eBioscience); APC-conjugated anti-CD86 (Tonbo Biosciences); PE-conjugated anti-CD69 (BioLegend); PE-conjugated anti-PD-1 (Tonbo Biosciences); Brilliant Violet 421^™-conjugated anti-C-X-C chemokine receptor type 5 (BioLegend); Alexa Fluor 647-conjugated anti-inducible T cell co-stimulator (BioLegend); eFour450-conjugated anti-CD127 (eBioscience); PE-conjugated anti-CD25 (Tonbo Biosciences); and APC-conjugated anti-CTLA4 (Tonbo Biosciences). In addition, dead cells were excluded by propidium iodide staining. FlowJo (FlowJo, LLC) was used to conduct data analysis.