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Coulter ac t diff2 hematology analyzer

Manufactured by Beckman Coulter
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The COULTER® Ac·T diff2™ Hematology Analyzer is a compact automated laboratory instrument designed for routine complete blood count (CBC) and 3-part differential analysis of human blood samples. The analyzer utilizes the Coulter principle of cell counting and sizing to provide accurate and reliable hematology data.

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Hematology analyzer (30 citations) Ac∙T diff2 Analyzer (2 citations)

21 protocols using coulter ac t diff2 hematology analyzer

1

Blood Cell Differential Analysis

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Blood differential cell counts were carried out on fresh whole blood collected in EDTA rather than heparin tubes to avoid heparin-induced aggregation of platelets [14 (link)], using a Coulter Ac•T diff2TM Hematology Analyzer (Beckman-Coulter Corp., Miami, FL).
Elisia I., Lam V., Cho B., Hay M., Li M.Y., Kapeluto J., Elliott T., Harris D., Bu L., Jia W., Leung H., Mohn W, & Krystal G. (2020). Exploratory examination of inflammation state, immune response and blood cell composition in a human obese cohort to identify potential markers predicting cancer risk. PLoS ONE, 15(2), e0228633.
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2

Differential Blood Cell Counting

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Blood differential cell counts were carried out on fresh whole blood collected in EDTA rather than heparin tubes to avoid heparin-induced aggregation of platelets [29 (link)], using a Coulter Ac•T diff2TM Hematology Analyzer (Beckman-Coulter Corp., Miami, FL).
Elisia I., Lam V., Hofs E., Li M.Y., Hay M., Cho B., Brooks-Wilson A., Rosin M., Bu L., Jia W, & Krystal G. (2017). Effect of age on chronic inflammation and responsiveness to bacterial and viral challenges. PLoS ONE, 12(11), e0188881.
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3

Inflammatory Marker Measurements in Fasting Blood

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Fasting blood samples were collected at the study centre and were stored at −80 °C until full blood count measurements. These measurements included absolute counts of granulocytes, lymphocytes and platelets and were performed using the COULTER® Ac·T diff2™ Hematology Analyzer (Beckman Coulter, San Diego, California, USA). In an additional analysis, the normal distribution of hemoglobin and CRP levels were assessed as well. CRP levels were measured using a particle enhanced immunoturbidimetric assay (Roche Diagnostics, Mannheim, Germany).
The neutrophil-to-lymphocyte ratio was calculated on the basis of absolute peripheral granulocyte (as a proxy for the neutrophil count) (N; ×109/Liter) and lymphocyte (L; ×109/Liter) blood counts, using the formula: NLR = N/L9 (link).
The platelet-to-lymphocyte ratio was calculated on the basis of peripheral platelet(P; ×109/Liter) and lymphocyte (L; ×109/Liter) blood counts, using the formula: PLR = P/L12 (link).
The systemic immune-inflammation index (SII) was calculated on the basis of peripheral platelet (P; ×109/Liter), granulocyte (N; ×109/Liter) and lymphocyte (L; ×109/Liter) blood counts, using the following formula: SII = P * N/L10 (link). All the inflammatory markers are either ratios or indices and as such do not have a unit.
Fest J., Ruiter R., Ikram M.A., Voortman T., van Eijck C.H, & Stricker B.H. (2018). Reference values for white blood-cell-based inflammatory markers in the Rotterdam Study: a population-based prospective cohort study. Scientific Reports, 8, 10566.
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4

Hematological Biomarkers for Disease Monitoring

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Fasting blood samples were taken during each visit at the research center with a maximum of three visits during follow-up. Full blood count measurements were performed using the COULTER® Ac·T diff2™ Hematology Analyzer (Beckman Coulter, San Diego, CA, USA) directly after blood sample drawn. Laboratory measurements included absolute granulocyte, platelet, and lymphocyte counts in 109 per liter.
Since neutrophil counts were not available, we used granulocyte count as a reliable proxy given that these are the most abundant subtype of neutrophils [21 , 22 (link)].
The granulocyte-to-lymphocyte ratio (GLR) and PLR were calculated as the ratio of granulocyte count to lymphocyte count and as the ratio of platelet count to lymphocyte count, respectively. The SII was defined as platelet count times the GLR.
van der Willik K.D., Fani L., Rizopoulos D., Licher S., Fest J., Schagen S.B., Ikram M.K, & Ikram M.A. (2019). Balance between innate versus adaptive immune system and the risk of dementia: a population-based cohort study. Journal of Neuroinflammation, 16, 68.
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5

Hematological Markers in Clinical Assessment

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Full blood count measurements were performed using the COULTER® Ac·T diff2™ Hematology Analyzer (Beckman Coulter, San Diego, California, USA) directly after the blood sample was drawn. Laboratory measurements included absolute granulocyte, platelet, and lymphocyte counts in 109/L. The GLR and PLR were calculated as the ratio of granulocyte count to lymphocyte count, and as the ratio of platelet count to lymphocyte count, respectively. The SII was defined as platelet count times the GLR.13 We use the GLR as a proxy measure for the commonly used neutrophil‐to‐lymphocyte ratio, as granulocytes are the most abundant subtype of neutrophils.
Fani L., Ahmad S., Ikram M.K., Ghanbari M, & Ikram M.A. (2020). Immunity and amyloid beta, total tau and neurofilament light chain: Findings from a community‐based cohort study. Alzheimer's & Dementia, 17(3), 446-456.
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6

Blood Cell Analysis in Trauma Rats

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Whole blood was collected at terminal time points of 24 h (n = 8/group) and 72 h (n = 6/group) post-trauma from a deeply anesthetized rat by cardiac puncture, followed by euthanasia. Complete blood cell counts were analyzed on a hematology analyzer (COULTER Ac•T diff2 Hematology analyzer, Beckman Coulter, Brea, Ca), for circulating concentration and composition of lymphocytes, monocytes, and granulocytes (n = 8/group 24 h post-injury; n = 7, 72 h post osteotomy; n = 6, 72 h post polytrauma; n = 8 naïve). Additional aliquots of blood were collected in EDTA tubes; plasma was isolated and stored at − 80 °C for analysis.
Mangum L.H., Avila J.J., Hurtgen B.J., Lofgren A.L, & Wenke J.C. (2019). Burn and thoracic trauma alters fracture healing, systemic inflammation, and leukocyte kinetics in a rat model of polytrauma. Journal of Orthopaedic Surgery and Research, 14, 58.
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7

Blood Collection and Processing for Post-Surgical Analysis

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At 4- or 24-hour post-surgery, whole blood was collected via cardiac puncture and transferred to microcentrifuge tubes containing 100 μL of 3.2% sodium citrate anticoagulant at a volume ratio of 1:8 (anticoagulant: blood). Complete blood counts were performed using the Coulter Ac●T diff2 Hematology Analyzer (Beckman Coulter). For select experiments, blood from WT mice 24-hour post-surgery was processed into plasma via centrifugation. Blood was first spun at 1000 x g at room temperature for 10 minutes and supernatants transferred to new Eppendorf tube and centrifuged at 1000 x g at 4°C for 10 minutes. Supernatants were transferred to new Eppendorf tube and finally centrifuged at 10,000 x g at 4°C for 10 minutes and resultant plasma was stored at −80° C for future experiments.
Williams B., Zhu J., Zou L, & Chao W. (2022). Innate immune TLR7 signaling mediates platelet activation and platelet-leukocyte aggregate formation in murine bacterial sepsis. Platelets, 33(8), 1251-1259.
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8

Systemic Immune-Inflammation Index Protocol

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Fasting blood samples were collected at the study centre. Full blood counts were performed on a Coulter Ac.T diff2 Hematology Analyzer (Beckman Coulter, San Diego, CA, USA). The SII was calculated from the platelets (×109 cells·L−1), granulocytes, as a proxy for neutrophils (×109 cells·L−1), and lymphocytes (×109 cells·L−1), using the formula SII=platelets×neutrophils/lymphocytes [12 (link)]. Sex-specific quartiles of SII were defined for all participants.
Benz E., Wijnant S.R., Trajanoska K., Arinze J.T., de Roos E.W., de Ridder M., Williams R., van Rooij F., Verhamme K.M., Ikram M.A., Stricker B.H., Rivadeneira F., Lahousse L, & Brusselle G.G. (2022). Sarcopenia, systemic immune-inflammation index and all-cause mortality in middle-aged and older people with COPD and asthma: a population-based study. ERJ Open Research, 8(1), 00628-2021.
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9

Platelet Isolation and Preparation

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Citrated blood samples were allowed to rest at room temperature for 15 min post-phlebotomy. Platelet rich plasma (PRP) was obtained by centrifugation of blood at 200 g for 10 min. PRP was added to 1:10 (v/v) ACD-A [tris-sodium citrate (25 g/L), glucose (20 g/L), and citric acid (14 g/L)] and spun at 1000 g for 10 min. The platelet pellet was washed in Tyrode’s buffer [137 mM NaCl, 2.8 mM KCl, 1 mM MgCl2, 12 mM NaHCO3, 0.4 mM Na2HPO4, 5.5 mM glucose, 10 mM HEPES, pH 7.4] and 1 μM PGE1 (Sigma), prior to centrifugation at 1000 g for 10 min and resuspension in Tyrode’s buffer containing 2 mM CaCl2. Platelets were counted on a COULTER AC·T diff2 Hematology Analyzer (Beckman Coulter Inc, Brea, CA) and adjusted to the desired concentration.
Barrett T.J., Schlegel M., Zhou F., Gorenchtein M., Bolstorff J., Moore K.J., Fisher E.A, & Berger J.S. (2019). Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis. Science translational medicine, 11(517), eaax0481.
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10

Hematologic Biomarkers and Inflammation

Cited 1 time
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All participants had fasting blood samples taken during the research center visit. Full blood count measurements were performed by using a COULTER® Ac·T diff2™ Hematology Analyzer (Beckman Coulter, San Diego, CA, USA) directly after the blood sample was drawn. Hematologic measurements included absolute granulocyte, lymphocyte, and platelet counts in 109 per liter.
We used the granulocyte count as proxy for the neutrophil count because we did not have this measurement available in our sample. Because most of the granulocytes are represented by neutrophils, we believe this did not affect our results [26 , 27 (link)]. For accuracy purposes, we will refer to the granulocyte-to-lymphocyte ratio (GLR) instead of using the term NLR.
The GLR and PLR were calculated as the ratio of granulocyte count to lymphocyte count and as the ratio of platelet count to lymphocyte count, respectively [28 (link)]. The SII was defined as platelet count times the GLR [22 (link)]. Because they are either ratios or indices, the derived inflammatory markers did not have a unit.
van der Willik K.D., Koppelmans V., Hauptmann M., Compter A., Ikram M.A, & Schagen S.B. (2018). Inflammation markers and cognitive performance in breast cancer survivors 20 years after completion of chemotherapy: a cohort study. Breast Cancer Research : BCR, 20, 135.
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