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Hrp conjugated secondary antibodies against rabbit and mouse igg

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HRP-conjugated secondary antibodies against rabbit and mouse IgG are used in immunoassays and Western blotting to detect the presence of primary antibodies targeting rabbit or mouse immunoglobulin G (IgG). These secondary antibodies are conjugated with horseradish peroxidase (HRP), an enzyme that catalyzes a color-producing reaction, enabling visualization and quantification of the target antigen.

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Lab products found in correlation

Enhanced chemiluminescence, by Thermo Fisher Scientific (2 mentions) Antibody against human mouse abca1, by Thermo Fisher Scientific (2 mentions) Mouse anti p53 1c12, by Cell Signaling Technology (1 mentions) Anti parp antibody, by Cell Signaling Technology (1 mentions) Limk1, by Cell Signaling Technology (1 mentions) Nrbp1, by GeneTex (1 mentions) Phospho cofilin s3, by Cell Signaling Technology (1 mentions) Pak1 2 3, by Cell Signaling Technology (1 mentions) Limk2, by Cell Signaling Technology (1 mentions) Phospho limk1 thr508 limk2 thr505, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Cdc42, by Cell Signaling Technology (1 mentions) Luciferin, by Promega (1 mentions)

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HRP-conjugated antibodies (10 citations) HRP-conjugated secondary antibodies against mouse (2 citations)

4 protocols using hrp conjugated secondary antibodies against rabbit and mouse igg

1

Western Blot Analysis of Cholesterol Homeostasis

Cited 1 time
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Western blots were performed as described previously [40 (link)]. Antibodies against FDXR, p21, mevalonate decarboxylase (MVD), mevalonate kinase (MVK), and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against human/mouse ABCA1 was purchased from Invitrogen Life Technologies (Carlsbad, CA) and Cell signaling Technology (#96292, Danvers, MA). Antibodies against SREBP1 and SREBP2 were purchased from Abcam (Cambridge, MA). HRP-conjugated secondary Antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA). The immunoreactive bands were visualized by enhanced chemiluminescence (Thermo Fisher Scientific Inc, Carlsbad, CA) and photographed with the BioSpectrum® 810 Imaging System (UVP LLC, Upland, CA).
Zhang Y., Mohibi S., Vasilatis D.M., Chen M., Zhang J, & Chen X. (2022). Ferredoxin reductase and p53 are necessary for lipid homeostasis and tumor suppression through the ABCA1–SREBP pathway. Oncogene, 41(12), 1718-1726.
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2

Western Blot Analysis of Metabolic Regulators

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Western blots were performed as described previously (40 (link)). Antibodies against FDXR, p21, mevalonate decarboxylase (MVD), mevalonate kinase (MVK) and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against human/mouse ABCA1 was purchased from Invitrogen Life Technologies (Carlsbad, CA) and Cell signaling technology (#96292, Danvers, MA). Antibodies against SREBP1 and SREBP2 were purchased from Abcam (Cambridge, MA). HRP-conjugated secondary Antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA). The immunoreactive bands were visualized by enhanced chemiluminescence (Thermo Fisher Scientific Inc, Carlsbad, CA) and photographed with the BioSpectrum® 810 Imaging System (UVP LLC, Upland, CA).
Zhang Y., Mohibi S., Vasilatis D.M., Chen M., Zhang J, & Chen X. (2022). Ferredoxin reductase and p53 are necessary for lipid homeostasis and tumor suppression through the ABCA1–SREBP pathway. Oncogene, 41(12), 1718-1726.
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3

Western Blot Analysis of Protein Targets

Cited 1 time
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Western blot was performed as described [41 (link)]. Antibodies against p53, p21, Egr-1, IRP2 (The anti-IRP2 antibody used in Figs. 3–5 and Fig. 6 have different lot number), FTH1, TfR1, and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-mouse IRP2 antibody was a generous gift of Elizabeth Leibold (University of Utah) [53 (link)]. Anti-mouse p53 (1C12) and anti-PARP antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibodies against FDXR were purchased from Abcam (Cambridge, MA, USA). HRP-conjugated secondary antibodies against rabbit and mouse IgG were purchased from BioRad (Hercules, CA). The immunoreactive bands were visualized by enhanced chemiluminescence (Thermo Fisher Scientific Inc, Carlsbad, CA) and quantified by densitometry with the BioSpectrum® 810 Imaging System (UVP LLC, Upland, CA).
Zhang Y., Feng X., Zhang J., Chen M., Huang E, & Chen X. (2019). Iron regulatory protein 2 is a suppressor of mutant p53 in tumorigenesis. Oncogene, 38(35), 6256-6269.
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4

Characterization of P-Rex1 Signaling Pathway

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The following antibodies were purchased from Cell Signaling Technology: P-Rex1 (cat. 13168), Cdc42 (cat. 2462), Ki-67 (cat. 9027), Myc-tag (cat. 2276 S), PAK1/2/3 (cat. 2604), phospho-PAK1 (Thr423)/PAK2 (Thr402) (cat. 2601), LIMK1 (cat. 3842), LIMK2 (cat. 3845), phospho-LIMK1 (Thr508)/LIMK2 (Thr505) (cat. 3841), phospho-Cofilin (S3) (cat. 3313s) and cleaved PARP (cat. 9546s). Flag-tag (cat. F7425), P-Rex1 (cat. SAB2501302) and P-Rex1 (cat. HPA001927) were purchased from Sigma. β-actin (cat. sc-69879), pan 14-3-3 (cat. sc-1657), Cofilin (cat. sc-376476) and phospho-Cofilin (S3) (cat. sc-365882) were purchased from Santa Cruz Biotechnology. The following antibodies were also used: NRBP1 (Genetex, cat. GTX84007), Rac1 (EMD Millipore, cat. 05-389) and HRP-conjugated secondary antibodies against rabbit and mouse IgG (Bio-Rad, cat. 1706515, 1706516). Western blotting was undertaken as previously described [41 (link)].
N-acetylcysteine (NAC, cat. A7250) and mitomycin C (cat. M4287) were purchased from Sigma-Aldrich. Luciferin (Promega, cat. P1043) was used in animal work.
Yang X., Cruz M.I., Nguyen E.V., Huang C., Schittenhelm R.B., Luu J., Cowley K.J., Shin S.Y., Nguyen L.K., Lim Kam Sian T.C., Clark K.C., Simpson K.J., Ma X, & Daly R.J. (2023). The pseudokinase NRBP1 activates Rac1/Cdc42 via P-Rex1 to drive oncogenic signalling in triple-negative breast cancer. Oncogene, 42(11), 833-847.
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