The OPE was characterized using liquid chromatography–mass spectrometry (LC/MS, 1260 Infinity LC, and 6410 Triple Quadrupole MS, Agilent Technologies, USA) to detect the phenolic compounds in the onion peel. One g of dried extract powder was dissolved in 10 mL of Milli-Q water (resistivity: 18.2 MΩ cm), and kept in the ultrasonic bath for 15 min. Then, the solution was filtered using a nylon filter (0.2 μm) and diluted. The sample was passed through the ZORBAX C18 column (2.1 × 50 mm2, 3.5 μm) column (maintained at 35 °C) using mobile phase A (water + 0.1% formic acid) and mobile phase B (100% water) at a flow rate of 200 µL min−1. The elution gradient profile was used as following: 2% B (0–3 min), 2–13% B (3–5 min), 13% B (5–9 min), 13–18% B (9–12 min), 18% B (12–13 min), 18–30% B (13–17 min), 30–50% B, (17–21 min), 50–98% B (21–22 min), 98% B (22–27 min). After elution, the mass spectra of the analytes were obtained by scanning m/z ratio from 50 to 950 in negative ionization mode at 300 °C of capillary temperature (Bedrníček et al. 2020 (link); Celano et al. 2021 (link)).
6410 triple quadrupole ms
Manufactured by Agilent Technologies
Sourced in United States
The 6410 Triple Quadrupole MS is a high-performance mass spectrometer designed for sensitive and accurate quantitative analysis. It features a triple quadrupole configuration that enables precise detection and measurement of target analytes in complex matrices.
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Lab products found in correlation
1260 infinity lc, by Agilent Technologies (1 mentions)
1200 hplc system, by Merck Group (1 mentions)
Chromolith fastgradient rp 18e 50 2mm column, by Merck Group (1 mentions)
Optimizer, by Agilent Technologies (1 mentions)
Eclipse xdb c18, by Agilent Technologies (1 mentions)
1200 series, by Agilent Technologies (1 mentions)
5 protocols using 6410 triple quadrupole ms
1
Onion Peel Extract Phenolic Profiling
2
HPLC-MS/MS Quantification of Nucleotides
3
LC-MS Analysis of Biosurfactant Compounds
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The LC–MS analysis was conducted in negative ion mode using a Q-TOF Premier model from Waters. The dry biosurfactant was dissolved in an acetonitrile solution and subsequently injected into the LC–MS system, which consisted of a 1260 Infinity LC instrument coupled with a 6410 Triple Quadrupole MS from Agilent Technologies, USA. Mass spectra were acquired within the m/z range of 100–1000, following the procedures described by Goveas et al.30 (link) and Sharma et al.31 (link). In this study, the LC–MS analysis method employed an MS detector with an ESI ( +) ion source and an MS analyzer in the form of a Q-Tof, enabling the generation of chromatographic peaks. Subsequently, the chromatographic peaks were subjected to analysis using the Masslynx program, as detailed by Sommeng et al.32 (link).
4
Quantification of Antibiotics in Wastewater
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The targets, AZI, ERY, and ROX, in the WWTP influents and effluents were analyzed by high-performance liquid chromatography interfaced with tandem mass spectrometry (HPLC-MS/MS, Agilent 6410 triple quadrupole MS) that was equipped with an electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode. All three target antibiotics were analyzed in positive MRM mode. A 10-μL aliquot of extract was injected onto an Agilent Eclipse XDB-C18 (3.0 × 75 mm, 3.5 μm) column with a poroshell 120 precolumn filter (3.0 mm, 0.2 μm). The column temperature was kept at 40 °C for the analysis. The mobile phases 0.1% (v/v) formic acid (A) and acetonitrile (B) were used, under the following gradient conditions: 0 min, 5% B; 5 min, 40% B; 8 min, 80% B; 16 min, 95% B, and 19 min, 5% B. The flow rates of the mobile phases were 0.3 mL/min, and the mass spectrometry conditions were optimized using Optimizer (Agilent, USA) for the fine tuning of the fragmentor voltage, collision energy (CE) and MRM transitions for the AZI, ERY, and ROX. Nitrogen gas was used as the drying and collision gas. The following optimized parameters were selected: drying gas temperature 200 °C, drying gas flow rate 6 mL/min, and capillary voltage 4200 V. The system was re-equilibrated for 10 min between runs.
5
Phthalate Monoesters Quantification by LC-MS/MS
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Phthalate monoesters were quantified by liquid chromatography (1200 series -Agilent Technologies, Massy, France)tandem mass spectrometry (6410-triple quadrupole MS, in negative ion mode for the electrospray source -Agilent Technologies (LC-MS/MS), equipped with a silice upti-prep strategy column (100 A, C18-2, 2x100 mm, 2.2 μm -Interchim) heated at 40°C. The source was in ESI negative mode (N2: 350°C; gas flow: 660 L/h; capillary: 4000 V) and the injected sample volumes were 10 μL. The LC gradient flow, the MRM parameters for LC-MS/MS and limits of quantification (LOQs) are indicated in the Supplementary Material (Table SM.1, 2, 3 and 5).
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