Prostar 210 system

Manufactured by Agilent Technologies

Sourced in United States

The Prostar 210 system is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative applications. It features a dual-piston pump, a variable wavelength UV-Vis detector, and a manual or automated sample injection system. The Prostar 210 system is capable of performing isocratic and gradient elution, making it suitable for a wide range of chromatographic separations.

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Lab products found in correlation

3200 q trap, by Thermo Fisher Scientific (2 mentions) Inova 500 spectrometer, by Agilent Technologies (1 mentions) Ods a column, by YMC (1 mentions) Lcq fleet ion trap mass spectrometer, by Thermo Fisher Scientific (1 mentions) Kieselgel 60, by Merck Group (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions) Q gel 60 f254 plates, by Merck Group (1 mentions) Prostar 335, by Agilent Technologies (1 mentions) Sephadex lh 20, by Cytiva (1 mentions) Chirascan cd spectrometer, by Applied Photophysics (1 mentions) Lcq deca mass spectrometer, by Thermo Fisher Scientific (1 mentions) Hypersil bds c18 column, by Thermo Fisher Scientific (1 mentions) Q tof mass spectrometer, by Thermo Fisher Scientific (1 mentions) Mcp 300, by Anton Paar (1 mentions) Aviii 600 mhz nmr spectrometer, by Bruker (1 mentions) Nicolet is10 ft ir spectrometer, by Thermo Fisher Scientific (1 mentions) Microsorb mv c18 100 5 column, by Agilent Technologies (1 mentions) Ultrashield 400 plus spectrometer, by Bruker (1 mentions) Silica gel column, by Merck Group (1 mentions) Optimelt automated melting point system, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ProStar 210 (17 citations) ProStar system (17 citations) Prostar 210 HPLC system (5 citations)

6 protocols using prostar 210 system

Analytical Techniques for Natural Product Isolation

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Thin-layer chromatography was carried out using pre-coated silica Q-gel 60 F254 plates (0.25 mm, Merck). Column chromatography (CC) was conducted using silica gel (Kieselgel 60, 230–400 mesh, Merck), and Sephadex LH-20 (18–111 µm, GE Healthcare AB, Stockholm, Sweden). HPLC was performed using the Varian Prostar 210 system. A YMC-Pack ODS-A column (5 μm, 250 × 20 mm i.d., YMC, Kyoto, Japan) was used for preparative HPLC analysis with an 8 mL/min flow rate. ESI-MS was performed on an LCQ Fleet Ion Trap mass spectrometer (Thermo Scientific, Madison, WI, USA). NMR spectral data were acquired by using a Varian 500-MHz NMR spectrometer (Inova 500 Spectrometer, Varian, Palo Alto, CA, USA). Tetramethylsilane was used as an internal standard, and chemical shifts data were expressed as δ value.

Kim K.O., Lee D., Hiep N.T., Song J.H., Lee H.J., Lee D, & Kang K.S. (2019). Protective Effect of Phenolic Compounds Isolated from Mugwort (Artemisia argyi) against Contrast-Induced Apoptosis in Kidney Epithelium Cell Line LLC-PK1. Molecules, 24(1), 195.

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Synthesis and Peptide Conjugation of acPEO-b-PCL

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The end hydroxyl
group from synthesized acPEO was used to initiate the synthesis of
the acPEO-block-ε-caprolactone, acPEO-b-PCL, and acPEO-block-(α-benzyl
carboxylate-ε-caprolactone), acPEO-b-PBCL,
which was carried out by bulk ring-opening polymerization of ε-caprolactone
or BCL, respectively.^{33 (link)} For peptide conjugation,
micelles were prepared, by cosolvent evaporation, at a block copolymer
concentration of 5 mg/mL. The pH was then adjusted to 2.0 using HCl
(0.5 M solution). Micelles were then incubated at room temperature
under stirring. After 2 h, the pH was readjusted to 7.4 with NaOH,
followed by buffering of the micellar solution using PBS (10×,
pH 7.4). An aqueous peptide solution in 1% DMSO was prepared and added,
under constant stirring, at a peptide:polymer ratio (mol/mol) of 1:3.
After 2 h reaction, NaBH₃CN was added, and the reaction
was left for 24 h at room temperature under constant stirring. The
resulting micellar solution was extensively dialyzed against distilled
water and lyophilized. The molar conjugation percent of GE11 peptide
into the copolymers was determined by reverse-phase HPLC measuring
unreacted peptide concentration (Varian Prostar 210 system, Microsorb-MV
5 μm C18 100 Å column, a gradient of 0.1% trifluoroacetic
acid/acetonitrile).^{35 (link)}

de Paiva I.M., Vakili M.R., Soleimani A.H., Tabatabaei Dakhili S.A., Munira S., Paladino M., Martin G., Jirik F.R., Hall D.G., Weinfeld M, & Lavasanifar A. (2022). Biodistribution and Activity of EGFR Targeted Polymeric Micelles Delivering a New Inhibitor of DNA Repair to Orthotopic Colorectal Cancer Xenografts with Metastasis. Molecular Pharmaceutics, 19(6), 1825-1838.

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Carotenoid Extraction and Analysis in E. coli

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Carotenoids produced by E. coli cells or extracted from protein were analyzed with the help of a Nucleosil C18 4.6*250 column run at 1 mL min⁻¹ and column oven temperature set to 28 °C. For extraction, 100 μL of a protein sample or an aliquot of cell precipitate were mixed with 100 μL acetone and 100 μL hexane, then vortexed and centrifuged until phase separation was observed. The carotenoid-containing upper hexane fraction was dried under the gaseous nitrogen stream, redissolved in 25 μL acetone, and injected onto the C18 column. Carotenoid standards (ZEA, CAN, ECH, and βCar) were injected separately as premixes. The elution was followed by absorbance at 460 nm and the following gradient structure: 0–5 min 70% acetone in water, 5–25 min gradient of 70–>100% acetone in water, 25–30 min 100% acetone, 30–32 min 100–>70% acetone, then 70% acetone until 40 min. The HPLC runs were carried out on a Prostar 210 system (Varian) equipped with a Prostar 335 diode-array detector enabling to record the 240–900 nm absorbance spectrum of the carotenoids eluted.

Egorkin N.A., Dominnik E.E., Maksimov E.G, & Sluchanko N.N. (2024). Insights into the molecular mechanism of yellow cuticle coloration by a chitin-binding carotenoprotein in gregarious locusts. Communications Biology, 7, 448.

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Analytical Techniques for Natural Product Characterization

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NMR experiments were carried out on a Bruker AVIII 600 MHz NMR spectrometer (Bruker BioSpin GmbH company, Rheinstetten, Germany) (¹H 600 MHz, ¹³C 150 MHz), with tetramethylsilane as the internal standard. Optical rotations were recorded with an MCP 300 (Anton Paar, Shanghai, China) polarimeter at 28 °C. UV spectra were measured on a PERSEE TU-1900 spectrophotometer. IR spectra were carried out on a Nicolet Nexus 670 spectrophotometer, in KBr discs. CD spectra were measured on a Chirascan™ CD spectrometer (Applied Photophysics, London, UK). ESIMS spectra were recorded on a Finnigan LCQ-DECA mass spectrometer, and HRESIMS spectra were recorded on a Thermo Fisher Scientific Q-TOF mass spectrometer. Column chromatography (CC) was performed on silica gel (200–300 mesh, Qingdao Marine Chemical Factory, Qingdao, China) and Sephadex LH-20 (Amersham Pharmacia Biotech., Uppsala, Sweden). Thin-layer chromatography (TLC) was performed on silica gel plates (Qingdao Huang Hai Chemical Group Co., Qingdao, China, G60, F-254). The high-performance liquid chromatography (HPLC) separation was performed on a Varian Prostar 210 system equipped with a Prostar 320 UV detector on a preparative Hypersil C-18 BDS column (250 × 21.2 mm, L × ID, 5 μm Varian Dynamax, Thermo Fisher Scientific Inc., Waltham, MA, USA). All other chemicals used were analytical grade.

Huang S., Chen H., Li W., Zhu X., Ding W, & Li C. (2016). Bioactive Chaetoglobosins from the Mangrove Endophytic Fungus Penicillium chrysogenum. Marine Drugs, 14(10), 172.

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Purification and Characterization of Organic Compounds

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All solvents and reagents were purchased from either Sigma-Aldrich or Alfa Aesar and were used as received without further purification. Melting points were measured on a MPA100 OptiMelt automated melting point apparatus without correction. IR spectra were recorded on a Thermo Scientific Nicolet iS10 FT-IR spectrometer. Analytical thin-layer chromatography (TLC) analyses were carried out on Analtech Uniplate F254 plates, and FCC was performed over silica gel (230–400 mesh, Merck). ¹H (400 MHz) and ¹³C (100 MHz) nuclear magnetic resonance (NMR) spectra were recorded on a Bruker Ultrashield 400 Plus spectrometer, and chemical shifts were expressed in ppm. Mass spectra were obtained on an Applied BioSystems 3200 Q trap with a turbo V source for TurbolonSpray. Analytical reversed-phase high-performance liquid chromatography (HPLC) was performed on a Varian ProStar 210 system using the Agilent Microsorb-MV 100–5 C18 column (250 × 4.6 mm). All analyses were conducted at ambient temperature with a flow rate of 0.8 mL/min. Mobile phase is acetonitrile/water (90/10) with 0.1% trifluoroacetic acid (TFA). The UV detector was set up at 210 nm. Compound purities were calculated as the percentage peak area of the analyzed compound, and retention times (R_t) were presented in minutes. The purity of all newly synthesized compounds was identified as ≥95%.

Shahabuddin M., Toyoshima T., Aikawa M, & Kaslow D.C. (1993). Transmission-blocking activity of a chitinase inhibitor and activation of malarial parasite chitinase by mosquito protease.. Proceedings of the National Academy of Sciences of the United States of America, 90(9), 4266-4270.

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NMR-Based Characterization of Third Generation NAP Derivatives

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Reagents were purchased from either Sigma-Aldrich or Alfa Aesar. TLC analyses were carried out on the Analtech Uniplate F254 plates. Chromatographic purification was conducted on silica gel column (230–400 mesh, Merck). ¹H (400 MHz) and ¹³C (100 MHz) nuclear magnetic resonance (NMR) spectra were recorded at ambient temperature with tetramethylsilane as the internal standard on a Varian Mercury 400 MHz NMR spectrometer.
Melting points were recorded with OptiMelt automated melting point system (Fisher Scientific). IR spectra were obtained with Nicolet iS10 instrument (Thermo Scientific). Applied Bio Systems 3200 Q trap with a turbo V source for TurbolonSpray was used for MS analysis. HPLC analysis was performed by a Varian ProStar 210 system on Agilent Microsorb-MV 100–5 C18 (250 mm × 4.6 mm) at 210 nm with water/acetonitrile (25/75, 30/70 or 35/65, 0.01% TFA in water) at 0.8 mL/min over 30 min. The purity of the third generation NAP derivatives was determined by above analytical methods, and their purity was identified as ≥95%.

Zheng Y., Obeng S., Wang H., Jali A.M., Peddibhotla B., Williams D.A., Zou C., Stevens D.L., Dewey W.L., Akbarali H.I., Selley D.E, & Zhang Y. (2019). Design, Synthesis, and Biological Evaluation of the Third Generation 17-Cyclopropylmethyl-3,14β-dihydroxy-4,5α-epoxy-6β-[(4′-pyridyl)carboxamido]morphinan (NAP) Derivatives as μ/κ Opioid Receptor Dual Selective Ligands. Journal of medicinal chemistry, 62(2), 561-574.

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