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A301 985a m

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The A301-985A-M is a laboratory equipment product. It serves a core function of providing a controlled environment for scientific experiments or analysis. The specific details and intended use of this product are not available in an unbiased and factual manner.

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Lab products found in correlation

Ab56342, by Abcam (3 mentions) G8795, by Merck Group (3 mentions) Anti p27 sc 528, by Santa Cruz Biotechnology (2 mentions) Anti ha agarose beads a2095, by Merck Group (2 mentions) Peroxidase conjugated anti mouse secondary antibody a 4416, by Merck Group (2 mentions) Anti brd4 antibody, by Cell Signaling Technology (2 mentions) Anti gfp 8371 2 antibody, by Takara Bio (2 mentions) Erg antibody epr3864 2, by Abcam (2 mentions) Anti brd3 11859 1 ap antibody, by Proteintech (2 mentions) Polyclonal anti myc tag antibody 2278, by Cell Signaling Technology (2 mentions) Monoclonal anti myc tag 2276 antibodies, by Cell Signaling Technology (2 mentions) Polyclonal anti ha sc 805, by Santa Cruz Biotechnology (2 mentions) Anti xpress 46 0528 antibody, by Thermo Fisher Scientific (2 mentions) Peroxidase conjugated anti rabbit secondary antibody a 4914, by Merck Group (2 mentions) Monoclonal anti ha antibody mms 101p, by BioLegend (2 mentions) Irdye 680, by LI COR (1 mentions)

Spelling variants of this product

Anti-BRD4 (A301-985A-M) antibody (2 citations)

5 protocols using a301 985a m

1

Comprehensive Antibody Validation Protocol

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
Dai X., Gan W., Li X., Wang S., Zhang W., Huang L., Liu S., Zhong Q., Guo J., Zhang J., Chen T., Shimizu K., Beca F., Blattner M., Vasudevan D., Buckley D.L., Qi J., Buser L., Liu P., Inuzuka H., Beck A.H., Wang L., Wild P.J., Garraway L.A., Rubin M.A., Barbieri C.E., Wong K.K., Muthuswamy S.K., Huang J., Chen Y., Bradner J.E, & Wei W. (2017). Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. Nature medicine, 23(9), 1063-1071.
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2

Comprehensive Antibody Validation Protocol

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-ERG antibody [EPR3864 (2)] and (C-20) were purchased from Abcam and Santa Cruz, respectively. Anti-SPOP antibody (16750-1-AP), Anti-BRD2 (22236-1-AP) and anti-BRD3 (11859-1-AP) antibody were purchased from Proteintech. Anti-BRD4 antibody was purchased from Cell Signaling (13440) and Bethyl laboratories (A301-985A-M). Anti-Cullin 3 (2759), anti-GST (2625), anti-DEK (13962s), anti-SRC3 (2126), anti-Cleaved Caspase-3 (9664), anti-Cleaved PARP (5625), polyclonal anti-Myc-Tag antibody (2278) and monoclonal anti-Myc-Tag (2276) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA antibody (MMS-101P) was purchased from Biolegend. Anti-GFP (8371-2) antibody was purchased from Clontech. Anti-Cullin 1 (SC-17775), anti-AR (N-20), anti-TRIM24/TIF1α (C-4), polyclonal anti-HA (SC-805) and anti-p27 (SC-528) were purchased from Santa Cruz. Anti-Xpress (46-0528) antibody was purchased from Invitrogen.
Dai X., Gan W., Li X., Wang S., Zhang W., Huang L., Liu S., Zhong Q., Guo J., Zhang J., Chen T., Shimizu K., Beca F., Blattner M., Vasudevan D., Buckley D.L., Qi J., Buser L., Liu P., Inuzuka H., Beck A.H., Wang L., Wild P.J., Garraway L.A., Rubin M.A., Barbieri C.E., Wong K.K., Muthuswamy S.K., Huang J., Chen Y., Bradner J.E, & Wei W. (2017). Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4. Nature medicine, 23(9), 1063-1071.
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3

Quantification of BRD Protein Levels in HEK293T Cells

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HEK293T cells were seeded at 90% confluency in 12 well plates (353043, Falcon), left to attach for 1.5h, followed by the compound treatment for 5h. Primary and secondary antibodies used included anti-BRD4 at 1:1000 dilution (A301-985A-M, Bethyl Laboratories, Lot: 6), anti-BRD2 at 1:2,000 dilution (A302-582A, Bethyl Laboratories, Lot: 1), anti-BRD3 at 1:500 dilution (ab56342, Abcam, Lot: GR243549-8), anti-GAPDH at 1:10,000 dilution (G8795, Sigma, Lot: 065M4856V, Clone: GAPDH-71.1), IRDye680 Donkey anti-mouse at 1:10,000 dilution (926–68072, LiCor, Lot: C61116-05) and IRDye800 Goat anti-rabbit at 1:10,000 dilution (926–32211, LiCor, Lot: C70301-05).
Nowak R.P., DeAngelo S.L., Buckley D., He Z., Donovan K.A., An J., Safaee N., Jedrychowski M.P., Ponthier C.M., Ishoey M., Zhang T., Mancias J.D., Gray N.S., Bradner J.E, & Fischer E.S. (2018). Plasticity in binding confers selectivity in ligand induced protein degradation. Nature chemical biology, 14(7), 706-714.
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4

Quantification of BRD Protein Levels in HEK293T Cells

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HEK293T cells were seeded at 90% confluency in 12 well plates (353043, Falcon), left to attach for 1.5h, followed by the compound treatment for 5h. Primary and secondary antibodies used included anti-BRD4 at 1:1000 dilution (A301-985A-M, Bethyl Laboratories, Lot: 6), anti-BRD2 at 1:2,000 dilution (A302-582A, Bethyl Laboratories, Lot: 1), anti-BRD3 at 1:500 dilution (ab56342, Abcam, Lot: GR243549-8), anti-GAPDH at 1:10,000 dilution (G8795, Sigma, Lot: 065M4856V, Clone: GAPDH-71.1), IRDye680 Donkey anti-mouse at 1:10,000 dilution (926–68072, LiCor, Lot: C61116-05) and IRDye800 Goat anti-rabbit at 1:10,000 dilution (926–32211, LiCor, Lot: C70301-05).
Nowak R.P., DeAngelo S.L., Buckley D., He Z., Donovan K.A., An J., Safaee N., Jedrychowski M.P., Ponthier C.M., Ishoey M., Zhang T., Mancias J.D., Gray N.S., Bradner J.E, & Fischer E.S. (2018). Plasticity in binding confers selectivity in ligand induced protein degradation. Nature chemical biology, 14(7), 706-714.
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5

BRD Protein Expression Profiling

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Example 15

HEK293T cells were seeded at 90% confluency in 12 well plates (353043, Falcon), left to attach for 1.5 h, followed by the compound treatment for 5 h. Primary and secondary antibodies used included anti-BRD4 at 1:1000 dilution (A301-985A-M, Bethyl Laboratories), anti-BRD2 at 1:2,000 dilution (A302-582A, Bethyl Laboratories), anti-BRD3 at 1:500 dilution (ab56342, Abcam®), anti-GAPDH at 1:10,000 dilution (G8795, Sigma), IRDye® 680 Donkey anti-mouse at 1:10,000 dilution (926-68072, LiCor®) and IRDye800 Goat anti-rabbit at 1:10,000 dilution (926-32211, LiCor®).

US11505560B2. Heterobifunctional compounds with improved specificity (2022-11-22). DANA-FARBER CANCER INSTITUTE, INC. [US]. Inventors: Radoslaw P. Nowak [US], Eric S. Fischer [US], Nathanael S. Gray [US], Tinghu Zhang [US], Zhixiang He [US], Brian Groendyke [US].
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