All procedures were performed according to the Canadian Council on Animal Care Guidelines and were approved by the University of Alberta Animal Care and Use Committee (AUP2711). Male and female C57BL/6 mice, between 60 and 180 days old, were used for all experiments. Mice were group housed in a temperature‐controlled environment on a reverse 12‐hr light–dark cycle. NPY‐hrGFP mice (van den Pol et al., 2009 ) were obtained from Jackson labs (RRID: IMSR_JAX:008069).
Npy hrgfp mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
NPY-hrGFP mice are a genetic model organism developed by Jackson ImmunoResearch. These mice express a fusion protein consisting of neuropeptide Y (NPY) and humanized Renilla green fluorescent protein (hrGFP), allowing for the visualization of NPY-expressing cells.
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Lab products found in correlation
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Ghrelin, by Enzo Life Sciences (1 mentions)
Agrptm1 cre lowl j, by Jackson ImmunoResearch (1 mentions)
Sr59230a, by Abcam (1 mentions)
Agrp ires cre mice, by Jackson ImmunoResearch (1 mentions)
Tdtomato reporter mice, by Jackson ImmunoResearch (1 mentions)
Male c57bl 6 mice, by Orient Bio (1 mentions)
10 protocols using npy hrgfp mice
1
Mouse Behavioral Experiments with NPY-hrGFP
2
Genetic models for AgRP neuron study
3
Investigating Cold and Diet Effects on Mice
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All animal experimental protocols were approved by the Third Military Medical University Animal Care Committee (Chongqing, China) and were performed according to the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals (NIH publication no. 8023). A total of 56 mice (C57BL/6) were used in the study (28 mice for the in vivo investigations of energy intake, body weight, adipose tissue mass, glucose and insulin tolerance, and in situ hybridization (Fig. 1A ). In total, 28 mice were used for investigations of central c-fos expression, and 28 transgenic mice expressing green fluorescent protein (GFP) under the control of the NPY promoter (Npy-hrGFP mice, stock no. 006417, which had been obtained from the Jackson Laboratory, Bar Harbor, ME, USA and further bred in our facility.) were used to determine whether NPY neurons in NPY-GFP mice were activated by cold exposure and HFD. All mice were housed under conditions of controlled temperature (22°C) and illumination (12 h light-dark cycle, starting at 7 am) with ad libitum access to water and standard chow (Gordon's Specialty Stock Feeds, Yanderra, New South Wales, Australia). The standard chow diet provided 8% of energy from fat, 21% from protein and 71% from carbohydrates, with a total energy content of 10.88 kJ/g.
4
Visualizing NPY Neurons in Mice
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All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and followed NIH guidelines for the use and care of laboratory animals. All animals were kept on a 12 h day/night cycle with unrestricted access to food and water. To visualize NPY neurons, NPY-hrGFP mice were obtained from Jackson Laboratory (stock #006417) and were kept hemizygous for the hrGFP transgene by crossing with C57BL/6J mice (Jackson Laboratory; stock #000664; van den Pol et al., 2009 (link)). Mice used for experiments were aged P22-P69 to avoid possible changes to auditory structures as a result of age-related hearing loss due to the Cdh23ahl mutation present in C57BL/6J mice (Noben-Trauth et al., 2003 (link)). For all experiments, mice of both sexes were used.
5
Mouse Housing and Breeding Protocol
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All mice used for breeding and experiments in this study were housed in a light-dark (12 h on/off; lights on at 7:00 AM) and temperature-controlled environment with food and water available ad libitum in the KAIST facilities. Npy-hrGFP mice were obtained from the Jackson laboratory (#006417). For some patch clamp experiments GIRK2 KO mice [41 (link)], used with the permission from Dr. Markus Stoffel (ETH Zurich), were crossed with Npy-hrGFP mice. Agrp-ires-Cre mice (Jackson laboratory, #012899) were crossed with tdTomato reporter mice (Jackson laboratory, #007914), GIRK1flox/flox mice [22 (link)] or GIRK2flox/flox mice [23 (link)] for FISH, ISH, IHC, and in vivo metabolic experiments. Mice were fed standard NCD (Teklad global 18% protein 2018S, ENVIGO).
6
Dietary Obesity Model in Transgenic Mice
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Male C57BL/6 mice (8–10-weeks old) were obtained from Orient Bio. Animals were housed under temperature-controlled conditions (22 ± 1 °C), humidity (55 ± 5%), and subjected to a 12-h light/dark cycle with lights on from 8 a.m. with ad libitum access to food to 8 p.m. unless indicated otherwise. Thus, 8 a.m. is zeitgeber time 0 (ZT0). Mice were fed a standard diet (Agripurina) with free access to food and water unless indicated otherwise. To induce diet-induced obesity, mice were fed a high-fat diet (60% fat; Research Diets, D12492). Mice with tdTomato-labeled POMC neurons were generated by mating POMC-Cre mice (provided by Dr. Joel K. Elmquist, University of Texas Southwestern Medical Center) with mice carrying the reporter Rosa26-loxP-STOP-loxP-tdTomato allele (Jackson Laboratory, 007909). NPY-hrGFP mice (Jackson Laboratory, 006417) were used for the purpose of AGRP neuron labeling. Dr. Joshep S. Takahashi (University of Texas Southwestern Medical Center) provided PER2:LUC knock-in mice used in the SCN clock study. All procedures were approved by the Institutional Animal Care and Use Committee of the Asan Institute for Life Sciences under the project license 2012-11-024 (Seoul, Korea).
7
Visualizing Neuropeptide Y Neurons
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All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and were in accordance with the National Institutes of Health’s Guide for the Care and Use of Laboratory Animals. Mice were kept on a 12 h day/night cycle with ad libitum access to food and water. To visualize NPY neurons, we used either NPY-hrGFP mice (Jackson Laboratory, stock #006417) (van den Pol et al., 2009 (link)) in which NPYgfp neurons are identified by the expression of hrGFP or NPY-FlpO x Ai65F mice in which NPYflp neurons are identified by tdTomato expression by crossing Npy-IRES2-FlpO-D mice (Jackson Laboratory, stock #030211)(Daigle et al., 2018 (link)) with Ai65F mice (Jackson Laboratory, stock #032864)(Daigle et al., 2018 (link)). All mice were on a C57BL/6J background, and mice of both sexes were used for all experiments.
8
Transgenic Mouse Model for Neuropeptide Y
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All experimental procedures were carried out in accordance with international ethical guidelines and the National Institutes of Health Guide for the Care and Use of Laboratory Animals and approved by The Third Military Medical University Animal Care and Use Committee. Male C57BL/6J mice were purchased from the Animal Center of The Third Military Medical University (Chongqing, China). The transgenic NPY‐GFP reporter mice expressing Green Fluorescent Protein (GFP) under the control of the NPY promoter, were obtained from Jackson Laboratories (NPY‐hrGFP mice, stock no. 00617, referred to as NPY‐GFP mice hereafter, ME, USA), which have been characterized previously (van den Pol et al., 2009 (link)). All the mice were housed under a condition of controlled temperature (23 ± 2°C) and light (12 h:12 h light/dark cycle), and fed a standard chow diet with ad libitum access to water.
9
NPY-hrGFP Mouse Experiments
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All procedures were performed according to the Canadian Council on Animal Care Guidelines and were approved by the University of Alberta Animal Care and Use Committee (AUP2711). Male and female C57BL/6 mice, between 60-180 days old, were used for all experiments. Mice were group housed in a temperature-controlled environment on a reverse 12hour light-dark cycle. NPY-hrGFP mice (van den Pol et al., 2009) (link) were obtained from Jackson labs (RRID: IMSR_JAX:008069).
10
Visualizing NPY Neurons in Mice
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All experiments were approved by the University of Michigan Institutional Animal Care and Use Committee and followed NIH guidelines for the use and care of laboratory animals. All animals were kept on a 12-hour day/night cycle with unrestricted access to food and water. To visualize NPY neurons, NPY-hrGFP mice were obtained from Jackson Laboratory (stock #006417) and were kept hemizygous for the hrGFP transgene by crossing with C57BL/6J mice (Jackson Laboratory; stock #000664)(van den Pol et al., 2009) . Mice used for experiments were aged P22-P69 to avoid possible changes to auditory structures as a result from age-related hearing loss due to the Cdh23 ahl mutation present in C57BL/6J mice (Noben-Trauth et al., 2003) . For all experiments, mice of both sexes were used.
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