Uhplc q exactive system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The UHPLC-Q Exactive system is a high-performance liquid chromatography (HPLC) instrument coupled with a high-resolution, accurate-mass (HRAM) mass spectrometer. The system is designed to provide efficient separation and sensitive detection of a wide range of analytes in complex samples. It combines ultra-high-pressure liquid chromatography (UHPLC) technology with Orbitrap mass spectrometry to deliver high-resolution, accurate-mass data for both qualitative and quantitative analysis.

Automatically generated - may contain errors

Lab products found in correlation

Uhplc q exactive mass spectrometer, by Thermo Fisher Scientific (2 mentions) Progenesis qi, by Waters Corporation (1 mentions) Beh c18 column, by Waters Corporation (1 mentions)

Close spelling variants of this product

Q Exactive (1165 citations) UHPLC system (58 citations) Q-Exactive system (37 citations) UHPLC-Q Exactive HF-X system (16 citations) UHPLC/Q-Exactive (15 citations) Thermo UHPLC-Q Exactive HF-X system (5 citations)

10 protocols using uhplc q exactive system

Metabolite Profiling of Fecal Samples

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The supernatant was extracted from 200 mg of faeces and transferred to sample vials[19 (link)]. Two microlitres of a sample was separated with an HSS T3 column and used for LC-MS/MS analysis. Mass spectrometric data were collected using a UHPLC-Q Exactive system (Thermo Fisher Scientific, Waltham, MA, United States) with an electrospray ionization source operating in the positive- and negative-ion modes. Data acquisition was performed in the data-dependent acquisition mode.
The raw LC-MS/MS data were preprocessed using Progenesis QI (Waters Corporation, Milford, MA, United States) software. Internal standard peaks and false-positive peaks were removed from the data matrix, redundant signals were removed, and the peaks were pooled. In addition, the metabolites were searched and identified in the HMDB, KEGG and Metlin databases.
Metabolites detected in at least 80% of any set of samples were retained[20 (link)]. After filtering, the metabolite response intensity of the mass spectrum peaks was normalized using the sum-normalization method. Moreover, variables with a relative standard deviation > 30% relative to the quality control samples were removed, and log10 logarithmization was performed to obtain the final data matrix for subsequent analysis.

Zhao J.D., Sun M., Li Y., Yu C.J., Cheng R.D., Wang S.H., Du X, & Fang Z.H. (2023). Characterization of gut microbial and metabolite alterations in faeces of Goto Kakizaki rats using metagenomic and untargeted metabolomic approach. World Journal of Diabetes, 14(3), 255-270.

+ Open protocol

+ Expand

Bile Acid Analysis in Mouse Stomach

Check if the same lab product or an alternative is used in the 5 most similar protocols

The stomach contents were collected at once after the mice were sacrificed and shipped to Majorbio company (Shanghai, China) at -80°C for analysis of bile acids. The instrument platform for LC-MS (Liquid Chromatography-Mass Spectrometry) analysis is UHPLC-Q Exactive system of Thermo Fisher Scientific.

Zhao C., Mu M., Li X., Dong Z., Wang J., Yao C., Zheng J., Sun X, & Yu J. (2024). USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis. Frontiers in Immunology, 15, 1326137.

+ Open protocol

+ Expand

UHPLC-MS Quality Control of Qinghuang Decoction

Check if the same lab product or an alternative is used in the 5 most similar protocols

The standards including geniposide, chlorogenic acid, resveratrol, and polydatin were used as the quality control of QHD and the fingerprint spectrum was established by ultra-high performance liquid chromatography—mass spectrometry (UHPLC-MS) method. The chromatographic profile of QHD was shown in Supplementary Figure 1. The analysis was performed with a UHPLC-Q/Exactive system (Thermo, San Jose, CA, USA) equipped with a quaternary gradient pump, an autosampler and high-resolution mass spectrometry detector. The components were eluted with a gradient system consisting of acetonitrile (I) and water (II) in gradient (time, min/II%: 0/95, 18/5). The mass detector molecular weight was set in the range of 100~1,000 Da. The contents of chlorogenic acid, geniposide, polydatin, and resveratrol were 5.61, 6.17, 14.16, and 13.11 mg/ml in QHD, respectively (Supplementary Figure 1).

Peng J.H., Leng J., Tian H.J., Yang T., Fang Y., Feng Q., Zhao Y, & Hu Y.Y. (2018). Geniposide and Chlorogenic Acid Combination Ameliorates Non-alcoholic Steatohepatitis Involving the Protection on the Gut Barrier Function in Mouse Induced by High-Fat Diet. Frontiers in Pharmacology, 9, 1399.

+ Open protocol

+ Expand

LC-MS Analysis of Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

After sample processing, LC-MS was performed on a Thermo UHPLC-Q Exactive system. Chromatographic separation was performed at 25 °C on a BEH C18 -column (100 mm × 2.1 mm i.d., 1.7 μm; Waters, Milford, USA). Solvent A was 10 mM CH3COONH4 in ACN/H2O (1/1) (0.1% (v/v) formic acid) and Solvent B was 2 mM CH3COONH4 in ACN/IPA/H2O (10/88/2) (0.02% (v/v) formic acid). Injection volume was 2.0 μl, flow rate was 0.4 ml/min, and column temperature was set at 40 °C.
The Thermo UHPLC-Q Exactive Mass Spectrometer equipped with an electrospray ionization positive and negative ion modes was used for mass spectrometer detection.

Li J., Huang H., Fan R., Hua Y, & Ma W. (2023). Lipidomic analysis of brain and hippocampus from mice fed with high-fat diet and treated with fecal microbiota transplantation. Nutrition & Metabolism, 20, 12.

+ Open protocol

+ Expand

Luhong Granules: Herbal Remedy Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

All crude ingredients for Luhong granules were purchased from Shanghai Hua-Yu Chinese Herbs Co., Ltd. (Shanghai, China). The Luhong granules consisted of 30 g of Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao (lot# 20121209) (in TCM, the root of Astragalus membranaceus is used as crude drug), 30 g of Codonopsis pilosula (Franch.) Nannf. (lot# 20110503) (in TCM, the root of Codonopsis pilosula is used as crude drug), 20 g of Lepidium apetalum Willd (lot# 20111004) (in TCM, the mature seed of Lepidium apetalum Willd is used as crude drug), 9 g of Cervus nippon Temminck (lot# 20121022) (in TCM, the corner block for antlers of Cervus nippon Temminck is used as crude drug. In the spring and autumn seasons, the ossification corners are removed to the gums, and the corner pieces are taken out and dried), 9 g of Carthamus tinctorius L. (lot# 20120603) (in TCM, the dry flower of Carthamus tinctorius L. is used as crude drug), and 9 g of Cinnamomum cassia Presl (lot# 20120712) (in TCM, the dry branch of Cinnamomum cassia Presl is used as crude drug). For quality control, Luhong granules were analysed by a UHPLC-Q Exactive System (Thermo, San Jose, CA, USA) [10 (link)]. Vancomycin was purchased from Servier, Shanghai Source Liquid Biotechnology Co., Ltd. (1404-93-9).

Yang T., Qu H., Song X., Liu Q., Yang X., Xu J., Yang T., Lan Z., Sha W, & Zhou H. (2019). Luhong Granules Prevent Ventricular Remodelling after Myocardial Infarction by Reducing the Metabolites TMAO and LPS of the Intestinal Flora. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 8937427.

+ Open protocol

+ Expand

UHPLC-Q Exactive LC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UHPLC-Q Exactive system of Thermo Fisher Scientific was used as the instrument platform for LC–MS analysis. The mass spectrometric data were acquired using a Thermo UHPLC-Q Exactive Mass Spectrometer with an electrospray ionization (ESI) source, which could operate in both positive and negative ion modes. The chromatographic conditions, MS conditions, and data preprocessing and annotation referred to the literature^{24 (link)}.

Chen H., Huang S., Quan C., Chen Z., Xu M., Wei F, & Tang D. (2024). Effects of different colors of plastic-film mulching on soil temperature, yield, and metabolites in Platostoma palustre. Scientific Reports, 14, 5110.

+ Open protocol

+ Expand

UHPLC-Q Exactive LC-MS Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The instrument platform for LC-MS analysis is a Thermo Fisher Scientific UHPLC-Q Exactive system.

Jiang L., Li X., Wang S., Pan D., Wu X., Guo F., Mu D., Jia F, & Zhang M. (2024). Analysis of metabolic differences between Jiaosu fermented from dendrobium flowers and stems based on untargeted metabolomics. Heliyon, 10(5), e27061.

+ Open protocol

+ Expand

Mass Spectrometry Analysis of Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

The samples were analyzed by liquid chromatography, whereby a single component entered the ion source of the high-vacuum mass spectrometer for ionization. The mass spectrum is obtained by separation according to the mass-to-charge ratio (m/z). Finally, the qualitative and quantitative results of each sample were obtained via its mass spectrum data analysis. LC–MS platform used was the UHPLC-Q Exactive system (Thermo Fisher Scientific, Waltham, Massachusetts, United States; Huang et al., 2022 (link)).

Hu Y., Cai M., Chu W, & Hu Y. (2022). Dysbiosis of Gut Microbiota and Lipidomics of Content Induced by Dietary Methionine Restriction in Rice Field Eel (Monopterus albus). Frontiers in Microbiology, 13, 917051.

+ Open protocol

+ Expand

UHPLC-Q Exactive Metabolic Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UHPLC-Q Exactive system from Thermo Fisher Scientific was used for the LC-MS analysis.
The chromatographic conditions were as follows: An HSS T3 column (100 mm × 2.1 mm id, 1.8 µm) was used for chromatography. Mobile phase A was 95% water + 5% acetonitrile (containing 0.1% formic acid), and mobile phase B was 47.5% acetonitrile + 47.5% isopropyl alcohol + 5% water (with 0.1% formic acid). The flow rate was 0.40 mL/min, the injection volume was 2 μL, and the column temperature was 40°C.
Electrospraying was used to ionize the materials for mass spectrometry (MS), and positive and negative ion scanning modes were used to acquire the mass spectrum signals of the samples, with a mass scanning range of 70 to 1,050 m/z. The operating parameters used were as follows: positive ion voltage, 3500 V; negative ion voltage, 2,800 V; sheath gas, 40 psi; auxiliary heating gas, 10 psi; ion source heating temperature, 400°C; 20-40-60 V cyclic collision energy; primary MS (MS1) resolution, 70,000; and secondary MS (MS2) resolution, 17,500.

Zhang L., Wang Y., Jia H., Liu X., Zhang R, & Guan J. (2022). Transcriptome and metabolome analyses reveal the regulatory effects of compound probiotics on cecal metabolism in heat-stressed broilers. Poultry Science, 102(1), 102323.

+ Open protocol

+ Expand

Metabolomics of Coffee Bean Fermentation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Metabolites of coffee beans during fermentation processing were extracted and analyzed using the liquid chromatography-mass-spectrometry (LC-MS/MS)-based metabolomics approach by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). The coffee powder (CP) samples (50 mg) were accurately weighed and extracted using 0.4 mL 80% methanol solution with 0.02 mg/mL L-2-chlorophenylalanin as the internal standard. Quality control (QC) samples were prepared by mixing equal volumes of all samples to monitor the stability of the analysis [34 (link)].
Samples were injected into a UHPLC-Q Exactive system of Thermo Fisher Scientific for LC-MS analysis [35 (link)]. LC-MS data were preprocessed by Progensis QI software 3.0 (Waters Corporation, Milford, MA, USA) [36 (link)]. At the same time, the metabolites were searched and identified by the HMDB, Metlin, and Majorbio Database [37 (link)]. The response intensity of the sample mass spectrum peaks was normalized by the sum normalization method, and variables with relative standard deviation (RSD) > 30% of QC samples were removed, and log10 logarithmization was performed.

Shen X., Wang B., Zi C., Huang L., Wang Q., Zhou C., Wen W., Liu K., Yuan W, & Li X. (2023). Interaction and Metabolic Function of Microbiota during the Washed Processing of Coffea arabica. Molecules, 28(16), 6092.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!