Dapagliflozin was purchased from the MedChemExpress Bio-Technology company (HY-10,450). A 10 mM stocking solution was dissolved and stored at -80˚C. RPMI 1640 medium and foetal bovine serum (FBS) were purchased from Gibco, Invitrogen (Carlsbad, CA, USA). Trypsin/EDTA was purchased from HyClone (Logan, UT, USA). WST-8 cell proliferation and cytotoxicity assay kit was purchased from Dojindo (CK04, Kumamoto, Japan). A cholesterol detection assay kit was purchased from Beijing Applygen Technologies (E-1015). The phalloidine staining reagent was purchased from the Sigma-Aldrich company. Nile acid was purchased from Macklin Inc (49,409). The siRNA specific for KLF-5 was designed and provided by the Genomeditech Company (Shanghai). Reagents for real-time PCR were purchased from Takara.
Primary antibodies are detailed as follows: Anti-ABCA1 (Abcam, Cambridge, MA, UK) for WB and IHC. Anti-KLF 5 (Abclonal, China) for WB and IHC. Anti-podocin (Abcam, UK); anti-nephrin (Abcam, UK); anti-LDLR (Abcam, UK); Anti-HMGCR (Abcam, UK); Anti-podocin (Abcam, UK); anti-Bax (Abcam, UK); anti-Bcl-2 (Abcam, UK); anti-caspase 3 (Abcam, UK); and, anti-GAPDH (Proteintech, China). Rats were purchased from the Animal Experimental Centre of Shandong University. All other materials and reagents were endotoxin-free and supplied by the central lab of Shandong Provincial Hospital.
Primary antibodies are detailed as follows: Anti-ABCA1 (Abcam, Cambridge, MA, UK) for WB and IHC. Anti-KLF 5 (Abclonal, China) for WB and IHC. Anti-podocin (Abcam, UK); anti-nephrin (Abcam, UK); anti-LDLR (Abcam, UK); Anti-HMGCR (Abcam, UK); Anti-podocin (Abcam, UK); anti-Bax (Abcam, UK); anti-Bcl-2 (Abcam, UK); anti-caspase 3 (Abcam, UK); and, anti-GAPDH (Proteintech, China). Rats were purchased from the Animal Experimental Centre of Shandong University. All other materials and reagents were endotoxin-free and supplied by the central lab of Shandong Provincial Hospital.