Ethylenediaminetetraacetic acid tetrasodium salt hydrate

Ethylenediaminetetraacetic acid tetrasodium salt hydrate (≥99.0%) (tEDTA), calcium chloride dihydrate, magnesium chloride hexahydrate (≥99.0%), sodium chloride (≥99.0%), sodium sulfate (≥99.0%), trisodium citrate dihydrate (≥99.0%), potassium chloride (≥99.0%), ammonium chloride (≥99.5%) and urea were obtained from Sigma-Aldrich (Dorset, UK). Creatinine (98%), sodium oxalate (≥99.5%) and potassium dihydrogen phosphate (≥98.0%) were purchased from Alfa Aesar (Heysham, UK). Quarter-strength Ringer's solution (QSRS), phosphate-buffered saline (PBS), tryptone soya broth (TSB), Mueller-Hinton broth (MHB) and agar were purchased from Oxoid Ltd (Hampshire, UK). BARD silicone, 14-channel male catheters and URIPLAN 2 L drainable bed bags (98 cm inlet) were purchased from BARD Ltd (Crawley, UK). Proteus mirabilis ATCC 51286 and Staphylococcus aureus ATCC 29213 were purchased from LGC Standards (Middlesex, UK), and Escherichia coli NSM59 was obtained from Dr Jones (University of Bath, UK). Deionised water used in bulk crystallisation assays was purified with an Aqua Solutions RODI-C-12A purification system (18.2 MΩ).

Mouse histamine monoclonal antibody (MBS2025715)
and histamine-BSA conjugate antigen (MBS358205) were purchased from
Mybiosource. Protein G was purchased from GenScript. β-parvalbumin
monoclonal antibody (PV235 PUR) was purchased from Swant. Bovine serum
albumin (BSA, ≥ 98%), casein sodium salt from bovine milk,
sucrose (99.5%), sodium phosphate monobasic (≥98%), Tween 20,
boric acid (99.5%), iron(II) sulfate heptahydrate (≥99%), silver
nitrate (≥99%), sodium citrate tribasic dihydrate (≥98%),
ethylenediaminetetraacetic acid tetrasodium salt hydrate (EDTA, 99%),
rhodamine B isothiocyanate (RBIT), and phosphate-buffered saline (PBS
10×) were purchased from Sigma-Aldrich. Hydrogen tetrachloroaurate
(III) trihydrate (99.99%) was supplied by Alfa Aesar. Sulfuric acid
(95–97%) was supplied by Scharlau. Citric acid monohydrate
(99.5%) and sodium phosphate dibasic acid (≥99%) were obtained
from Fluka. Malachite green isothiocyanate (MGITC) was purchased from
Invitrogen. Nitrocellulose membranes (UniSart CN95) were purchased
from Sartorius. Absorbent pads (CF6) and backing cards (10547158)
were purchased from Cytiva. Parvalbumin antigen was isolated at the
Marine Research Institute (IIM), CSIC, Vigo. All chemicals were used
as received, and ultrapure water (type I) was used in all the preparations.