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Nanodrop 2000c

Manufactured by Hellma
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Sourced in United States

The Nanodrop 2000c is a spectrophotometer designed for the quantification and analysis of nucleic acid and protein samples. It utilizes a small sample volume, typically 1-2 microliters, to measure the absorbance of the sample across a wide range of wavelengths. The instrument provides accurate and reliable measurements of sample concentration and purity.

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Lab products found in correlation

1.0 cm quartz cuvette, by Hellma (3 mentions) Prism 8, by GraphPad (1 mentions) Lyophilized fmoc ff powder, by Bachem (1 mentions) Span 60, by Merck Group (1 mentions) Tween 60, by Merck Group (1 mentions) Spark 20m, by Tecan (1 mentions)

5 protocols using nanodrop 2000c

1

UV/Vis Spectroscopy of Photosensitive Samples

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UV/Vis spectroscopy was performed on either: i) a JASCO V-770 UV/Vis/NIR Spectrophotometer (PbS-version) with a PAC-743 Peltierthermo 6/8 sample switching unit and a Julabo F-250 cooling system, using a Hellma quartz glass cuvettes (10 mm pathlength) and light for switching was delivered by a TILL Polychrome V coupled to a fiber optic (light intensities~ 1–2 mW/cm2); or ii) a NanoDrop 2000c using a Hellma quartz glass cuvettes (10 mm pathlength) and light for switching was delivered by a CoolLED pE-4000 (intensities used: I385 nm = 0.459 mW/mm2; I405 nm = 0.798 mW/mm2; P500 nm = 8.20 mW/mm2; P525 nm = 4.95 mW/mm2). Full spectra were acquired either in the dark or under appropriate illumination, and kinetic traces were recorded at one wavelength under annotated illumination. Results were exported and plotted in GraphPad Prism 8 and kinetic traces were fitted monoexponentially using IgorPro or GraphPad Prism 8 and plotted in GraphPad Prism 8. For the determination of extinction coefficients, see Supplementary Information.
Gutzeit V.A., Acosta-Ruiz A., Munguba H., Häfner S., Landra-Willm A., Mathes B., Mony J., Yarotski D., Börjesson K., Liston C., Sandoz G., Levitz J, & Broichhagen J. (2021). A fine-tuned azobenzene for enhanced photopharmacology in vivo. Cell chemical biology, 28(11), 1648-1663.e16.
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2

Peptide Solutions Preparation and Characterization

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2Nal2 and Gd-2Nal2 peptide solutions were prepared by dissolving the lyophilized powder in double distilled water. The concentration of the final solution was determined by absorbance on UV-vis Thermo Fisher Scientific Inc (Wilmington, Delaware USA) Nanodrop 2000c spectrophotometer using a 1.0 cm quartz cuvette (Hellma). The molar absorptivity (ε280) for 2Nal2 and Gd-2Nal2 peptide employed for the spectroscopic measurements was 6070 M−1 cm−1.
Diaferia C., Gianolio E., Sibillano T., Mercurio F.A., Leone M., Giannini C., Balasco N., Vitagliano L., Morelli G, & Accardo A. (2017). Cross-beta nanostructures based on dinaphthylalanine Gd-conjugates loaded with doxorubicin. Scientific Reports, 7, 307.
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3

Peptide Hydrogel Formation and Characterization

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Peptide
solutions were prepared by direct dissolution of pure powders in bidistilled
water. The analytical concentration of solutions was spectroscopically
determined by absorbance on a UV–vis Thermo Fisher Scientific
Inc. (Wilmington, Delaware, USA) Nanodrop 2000c spectrophotometer
equipped with a 1.0 cm quartz cuvette (Hellma) using the molar absorptivity
(ε) at 280 nm reported in Table 1. For each peptide, the ε was calculated using
the formula ε(280)= [x·5500
+ y·1215 + k·2630] [L·cm–1·mol–1], where x, y, and k were the numbers of
W, Y, and Dopa residues, respectively. The solubility values analytically
determined were 1.15, 3.77, 7.61, and 15.00 mg/mL for (WY)3, PEG8-(WY)3,
(W-Dopa)3, and PEG8-(W-Dopa)3, respectively. Hydrogel formation was
triggered using the “solvent switch” method, which consists
of the addition of water to a peptide stock solution (100 mg/mL in
DMSO). The peptide concentration after water addition was 1.0 wt %
(10.0 mg/mL).
Balasco N., Altamura D., Scognamiglio P.L., Sibillano T., Giannini C., Morelli G., Vitagliano L., Accardo A, & Diaferia C. (2024). Self-Assembled Materials Based on Fully Aromatic Peptides: The Impact of Tryptophan, Tyrosine, and Dopa Residues. Langmuir, 40(2), 1470-1486.
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4

Synthesis and Characterization of Pegylated Liposomal Doxorubicin

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Protected Nα-Fmoc-amino acid derivatives, coupling reagents, and Rink amide MBHA (4-methylbenzhydrylamine) resin were purchased from Calbiochem-Novabiochem (Laufelfingen, Switzerland). The monodisperse Fmoc-8-amino-3,6-dioxaoctanoic acid (Fmoc- AdOO-OH, PEG2) was purchased from Neosystem (Strasbourg, France). Lyophilized Fmoc-FF powder was purchased from Bachem (Bubendorf, Switzerland). Doxorubicin chlorohydrate (Dox.HCl) was purchased from Sigma Aldrich (Milan, Italy). Pegylated liposomal Dox (commercial name of Doxil) vials were kindly gifted by the Italian Cancer Institute in Naples (Italy) “Fondazione G. Pascale”. TWEEN®60, SPAN®60, and all other chemicals and solvents were purchased from Sigma-Aldrich, Fluka (Bucks, Switzerland) or LabScan (Stillorgan, Dublin, Ireland) and were used as received unless otherwise stated. All solutions were obtained by weight using doubly distilled water as a solvent. UV-Vis spectra were recorded on a Thermo Fisher Scientific Inc (Wilmington, Delaware USA) Nanodrop 2000c, equipped with a 1.0 cm quartz cuvette (Hellma).
Gallo E., Diaferia C., Rosa E., Smaldone G., Morelli G, & Accardo A. (2021). Peptide-Based Hydrogels and Nanogels for Delivery of Doxorubicin. International Journal of Nanomedicine, 16, 1617-1630.
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5

Spectrophotometry and Microplate Assays

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A Thermo
Scientific NanoDrop 2000c spectrophotometer equipped with an ultra-micro-cuvette
105.202-QS SD 10 mm from Hellma Analytics was used for UV–vis
spectroscopy measurements. QUANTI-Blue secreted alkaline phosphatase
and MTT assay readouts were performed on a Spark 20M multimode microplate
reader from TecanTrading AG (Mannedorf, Switzerland).
Scherger M., Pilger Y.A., Stickdorn J., Komforth P., Schmitt S., Koynov K., Räder H.J, & Nuhn L. (2023). Efficient Self-Immolative RAFT End Group Modification for Macromolecular Immunodrug Delivery. Biomacromolecules, 24(5), 2380-2391.
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