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Kmc2281

Manufactured by Thermo Fisher Scientific
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The KMC2281 is a laboratory equipment manufactured by Thermo Fisher Scientific. It is designed for the purpose of centrifugation. The core function of this product is to separate components of a liquid mixture based on their density and other physical properties.

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Lab products found in correlation

Ab108785, by Abcam (1 mentions) Glycerol assay kit, by Merck Group (1 mentions)

4 protocols using kmc2281

1

Adiponectin and Leptin ELISA Assay

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Serum, culture media and protein lysates samples were analyzed for adiponectin and leptin using an adiponectin mouse ELISA kit (ab108785, Abcam) and leptin mouse ELISA kit (KMC2281, Invitrogen).
Schaar A., Sun Y., Sukumaran P., Rosenberger T.A., Krout D., Roemmich J.N., Brinbaumer L., Claycombe-Larson K, & Singh B.B. (2019). Ca2+ entry via TRPC1 is essential for cellular differentiation and modulates secretion via the SNARE complex. Journal of Cell Science, 132(13), jcs231878.
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2

Maternal Diet Effects on Hypothalamic Ciliogenesis

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To study the effects of maternal diets on hypothalamic ciliogenesis in pups, dams were fed with a CD, HFD, or LPD from mating or from delivery until weaning. The litter size of each diet group was matched to exclude overfeeding or underfeeding (six per dam). Pups were sacrificed at P14 for the cilia analysis and DQ-BSA studies at P11. In separate analysis, pups were breastfed by dams receiving CD, HFD, or LPD feeding, injected with leptin from P4 to P13, and sacrificed at P14 for the hypothalamic cilia and lysosomal analysis. Plasma leptin concentrations in the offspring were measured at P10 by using leptin enzyme-linked immunosorbent assay (Invitrogen, #KMC2281).
Lee C.H., Song D.K., Park C.B., Choi J., Kang G.M., Shin S.H., Kwon I., Park S., Kim S., Kim J.Y., Dugu H., Park J.W., Choi J.H., Min S.H., Sohn J.W, & Kim M.S. (2020). Primary cilia mediate early life programming of adiposity through lysosomal regulation in the developing mouse hypothalamus. Nature Communications, 11, 5772.
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3

Plasma Cytokine and Leptin Analysis

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Plasma was subjected to multiplex analyses for cytokines (U-Plex Mouse Biomarker K15069L-1/298782, Mesoscale Diagnostics, Rockville, MD). Leptin concentration was assessed by ELISA (KMC2281, Invitrogen, ThermoFisher Scientific).
Lewis E.D., Williams H.C., Bruno M.E., Stromberg A.J., Saito H., Johnson L.A, & Starr M.E. (2022). Exploring the Obesity Paradox in a Murine Model of Sepsis: Improved Survival Despite Increased Organ Injury in Obese Mice. Shock (Augusta, Ga.), 57(1), 151-159.
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4

Plasma Adiponectin, Leptin, and Resistin

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Plasma adiponectin, leptin and resistin levels were determined with Novex mouse ELISA kits KMP0041, KMC2281 and EMRETN (Invitrogen), respectively, according to manufacturer's protocol. Fasting insulin from plasma samples was determined with Insulin Mouse Serum Assay (Cisbio). Plasma glycerol levels were measured using Glycerol Assay Kit (Sigma MAK117) according to instructions.
Karpale M., Kummu O., Kärkkäinen O., Lehtonen M., Näpänkangas J., Herfurth U.M., Braeuning A., Rysä J, & Hakkola J. (2023). Pregnane X receptor activation remodels glucose metabolism to promote NAFLD development in obese mice. Molecular Metabolism, 76, 101779.
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