Mda content kit

The shells of Camellia oleifera were collected from Camellia oleifera based in Tianquan County, Ya’an City, Sichuan Province, China, in 2020. The shells were dried at 60 °C for 4 days and then grounded into powder (60 sieves).
Manganese chloride, ethanol, sodium chloride, magnesium sulfate, calcium chloride, etc. were from Chengdu Kelong Chemical Co., Ltd., Chengdu, China. Peptone, tryptone, and yeast extract were purchased from OXOID company. Fetal bovine serum (FBS), DMEM high-glucose medium, digestive enzymes, phosphate-buffered saline (PBS), and penicillin–streptomycin solution (10X) were bought from Hyclone Company, Logan, UT, USA. Methyl thiazolyl tetrazolium (MTT), caspase-3, caspase-9, and reactive oxygen species (ROS) detection kits were obtained from Beyotime Biotechnology Company, Shanghai, China. MDA content kit and antioxidant enzyme kit were purchased from Nanjing Jiancheng Bioengineering Institute Company, Nanjing, China. Reverse transcription and fluorescence quantitative kits were purchased from Takara Biotechnology Company, Dalian, China.

MDA is the end product of reactive oxygen species (ROS)-induced peroxidation of cell membrane lipids, which are reliable markers of oxidative stress. MDA content was measured using the thiobarbituric acid method using an MDA content kit according to the manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute).

The mitoKATP channel agonist (DZX), antagonist (5-HD), and GJ inhibitor (CBX) were obtained from Sigma (St. Louis, MO, USA); PKC agonist (PMA) and PKC antagonist (Ro-31-8425) were obtained from Millpore (St. Louis, MO, USA); Cx43 (ab11369), p-Cx43 (ab30559), β-actin (ab8227) , GAPDH (ab8245) and VDAC-1 (ab34726) antibodies were obtained from Abcam (Cambridge, MA, USA); PKCε (sc-214) and p-PKCε (sc-12355) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); horseradish peroxidase-conjugated secondary antibodies were obtained from Bioss (bs-0293G-HRP, bs-0296G-HRP, Beijing, China); 2,3,5-triphenyltetrazolium chloride (TTC) were obtained from Sigma (St. Louis, MO, USA); In situ Cell Death Detection Kit was obtained from Roche Molecular Biochemicals (Mannheim, Germany); The SOD activity kit, MDA content kit, functional mitochondria isolation kit and mitochondrial protein extraction kit were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).