Total proteins were isolated by RIPA buffer (Solarbio, Beijing, China) and quantified using a NanoDrop 3000 (Thermo Fisher Scientific). After subjected to Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), protein samples were transferred onto polyvinylidene fluoride membranes (Millipore, Bradford, MA, USA). Next, 5% fat-free milk buffer was used to block the membranes for 2 h at 37 °C. And then primary antibodies rabbit-anti-HMGA2 (1:500; Cell Signaling Technology, Danvers, MA, USA) or rabbit-anti-human GAPDH (1:1000; Cell Signaling Technology) was used to incubate the membranes overnight at 4 °C. The membranes were then washed with TBST and incubated with secondary antibody marked goat anti-rabbit IgG horseradish peroxidase (HRP) (1:2000; Cell Signaling Technology). After washing with TBST, the blots were detected using an ECL detection kit (Pierce Biotech, Rockford, IL, USA) and analyzed by Image Pro-Plus 6.0 software.
Rabbit anti hmga2
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit-anti-HMGA2 is a primary antibody that specifically recognizes the High Mobility Group AT-Hook 2 (HMGA2) protein. HMGA2 is a non-histone nuclear protein that functions as an architectural transcription factor, modulating chromatin structure and gene expression.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Rabbit anti human gapdh, by Cell Signaling Technology (1 mentions)
Nanodrop 3000, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg horseradish peroxidase hrp, by Cell Signaling Technology (1 mentions)
Ripa buffer, by Solarbio (1 mentions)
Ab76013, by Abcam (1 mentions)
Ta 125 hdx, by Thermo Fisher Scientific (1 mentions)
Autostainer 360, by Thermo Fisher Scientific (1 mentions)
2 protocols using rabbit anti hmga2
1
Western Blot Analysis of HMGA2 Protein
2
Immunohistochemical Profiling of Lung Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sectioned organoids or tissues were stained with hematoxylin and eosin (H&E) or IHC-stained with the following antibodies: for organoids, anti-TTF1 (1:500; Abcam ab76013), rabbit anti-SFTPC (1:5000; Millipore ABC99), rabbit anti-Caveolin-1 (1:5000; Thermo C3237), rabbit anti-CCSP (1:5000; Millipore 07-623), and rabbit anti-Keratin 5 (1:5000; Biolegend 905501); for tissues, rabbit anti-TTF1 (1:500; Abcam ab76013) and rabbit anti-HMGA2 (1:400; Cell Signaling Technology 8179). For IHC staining of lung and organoid sections, antigen retrieval was performed in IHC retrieval solution (pH 6.0; Thermo TA250) for 20 min at 97°C. Slides were processed using a Thermo Scientific Autostainer 360 with the following run conditions: endogenous peroxidases (Thermo TA125H2O2q) blocking for 10 min, protein block (Biocare RBM96961L) for 30 min, primary antibody for 60 min, and labeled polymer (Biocare RMR622L) for 30 min with a 5-min DAB (Thermo TA125HDX) exposure.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!