For analysis of lipids, glucose, uric acid, creatinine and the inflammation marker high sensitivity C-reactive protein (hs-CRP), blood samples were drawn in the morning between 8:00 and 10:00 am after a period of overnight fasting. Serum levels of total cholesterol and high-density lipoprotein (HDL) cholesterol were measured with an enzymatic colorimetric method, low-density lipoprotein (LDL) cholesterol with an enzymatic method and total triglycerides with a colorimetric UV method, all on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). Fasting blood glucose was measured using a hexokinase method. HbA1c was determined in whole blood (EDTA-anticoagulated) by means of turbid metric inhibition immunoassay on a Cobas Integra 800 CTS analyzer (Roche Diagnostics Netherland BV, Almere, The Netherlands). Insulin resistance was measured by calculating the ratio total triglycerides/HDL cholesterol [27 (link)]. Serum uric acid and creatinine were measured on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). The hs-CRP was determined by nephelometry (BN II system Siemens, Marburg, Germany).
Cobas integra 800 cts analyzer
Manufactured by Roche
Sourced in Netherlands
The Cobas Integra 800 CTS analyzer is a laboratory instrument designed for performing a variety of clinical chemistry tests. It is capable of analyzing different types of samples, such as serum, plasma, and urine. The analyzer uses photometric and potentiometric measurement principles to determine the concentration of various analytes in the samples.
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Lab products found in correlation
6 protocols using cobas integra 800 cts analyzer
1
Comprehensive Metabolic and Inflammatory Profiling
2
Fasting Blood and Biochemical Analysis
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Blood samples were taken in the fasting state between 8.00 and 10.00 a.m. and transported to the LifeLines laboratory facility at room temperature or at 4 °C, depending on the sample requirements. On the day of collection, HbA1c (EDTA-anticoagulated) was analyzed using a NGSP-certified turbidimetric inhibition immunoassay on a Cobas Integra 800 CTS analyzer (Roche Diagnostics Nederland BV, Almere, the Netherlands). Serum creatinine was measured on a Roche Modular P chemistry analyzer (Roche, Basel Switzerland), and creatinine clearance was calculated with the chronic kidney disease epidemiology collaboration (CKD-EPI) formula [31 (link)]. Total and high density lipoprotein (HDL) cholesterol were measured using an enzymatic colorimetric method, triglycerides using a colorimetric UV method, and low density lipoprotein (LDL) cholesterol using an enzymatic method, on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). Fasting blood glucose was measured using a hexokinase method.
3
Comprehensive Cardiometabolic Biomarker Assessment
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During a second visit, which usually was 4–8 weeks later, blood was drawn between 8 and 10 a.m. while participants were in the fasting state. Biochemical measurements were performed on the same day. Glycated haemoglobin (HbA1c) was measured in EDTA-anticoagulated blood on a Cobas Integra 800 CTS analyzer (Roche Diagnostics, Almere, The Netherlands) with a National Glycohemoglobin Standardized Program certified turbidimetric inhibition immunoassay method. Blood glucose was measured with a hexokinase method. Serum concentrations of creatinine, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triacylglycerol were measured on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland. Estimated (e) GFR was calculated with the CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) formula16 (link).
4
Fasting Blood Biomarker Measurements
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Blood samples were collected in the fasting state, between 8.00 and 10.00 a.m, and transported on ice to the Central Lifelines Laboratory in the University Medical Center Groningen. Serum levels of total and HDL cholesterol were measured using an enzymatic colorimetric method, triglycerides using a colorimetric UV method, and LDL cholesterol using an enzymatic method, all on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). Fasting blood glucose was measured using a hexokinase method. HbA1c was determined in whole blood (EDTA-anticoagulated) by means of turbid metric inhibition immunoassay on a Cobas Integra 800 CTS analyzer (Roche Diagnostics Netherland BV, Almere, The Netherlands). The hs-CRP was determined by nephelometry (BN II system Siemens, Marburg, Germany). Serum creatinine was measured on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland).
5
Blood Biomarker Analysis Protocol
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Blood samples were collected in the morning after an overnight fast, directly into tubes containing heparin, and centrifuged. Measurements were performed in the clinical chemistry laboratory of the University Medical Center Groningen. Serum levels of total and high-density lipoprotein cholesterol were measured using an enzymatic colorimetric method, triglycerides using a colorimetric UV method, and low-density lipoprotein cholesterol using an enzymatic method, all on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland). HbA1c was measured using a turbidimetric inhibition immunoassay on a Cobas Integra 800 CTS analyzer (Roche Diagnostics Nederland BV, Almere, Netherlands). Fasting blood glucose was measured using a hexokinase method, and hs-CRP was analyzed using nephelometry (BN II system; Siemens, Marburg, Germany). Results of the thyroid hormone status were available in a subset of 4479 participants. TSH, fT4, and fT3 were assayed by electrochemiluminescent immunoassay on the Roche Modular E170 Analyzer using kits provided by the manufacturer (Roche, Basel, Switzerland) (27) . Normal values are 11-20 pmol/L for fT4 and 4.4-6.7 pmol/L for fT3. The general Dutch population is iodine sufficient. Tests to measure antithyroid peroxidase antibody levels were not performed.
6
Fasting Blood Biomarkers Measurement
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During a second visit, which usually was 4-8 weeks later, blood was drawn between 8 and 10 a.m. while participants were in the fasting state. Biochemical measurements were performed on the same day. Glycated haemoglobin (HbA 1c) was measured in EDTA-anticoagulated blood on a Cobas Integra 800 CTS analyzer (Roche Diagnostics, Almere, The Netherlands) with a National Glycohemoglobin Standardized Program certi ed turbidimetric inhibition immunoassay method. Blood glucose was measured with a hexokinase method. Serum concentrations of creatinine, total cholesterol, HDLcholesterol, LDL-cholesterol, and triacylglycerol were measured on a Roche Modular P chemistry analyzer (Roche, Basel, Switzerland. Estimated (e)GFR was calculated with the CKD-EPI (Chronic Kidney Disease Epidemiology Collaboration) formula [16] (link).
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