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Nova nano sem 450 instrument

Manufactured by Thermo Fisher Scientific
Sourced in United States

The Nova Nano SEM 450 is a high-resolution scanning electron microscope (SEM) designed for advanced imaging and analysis. It features a field emission gun (FEG) electron source, delivering high-quality, high-resolution images. The instrument is equipped with a wide range of analytical capabilities, including Energy-Dispersive X-ray Spectroscopy (EDS) and Electron Backscatter Diffraction (EBSD) detectors.

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5 protocols using nova nano sem 450 instrument

1

Fabrication and Characterization of Mixed Anode Materials

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LTO and LCO procured from Sigma-Aldrich
and commercially available HC were used as received. The mixed anodes
were prepared by taking LTO and HC in two proportions: 50–50
and 20–80 by weight. The slurry was made by mixing the anode
powders with Super P and polyvinylidene fluoride binder in the ratio
of 80:10:10 and N-methyl-2-pyrrolidone as the solvent.
The slurry was coated on the Cu foil and was kept for drying overnight
at 80 °C. The slurry was coated on the Cu foil and was kept for
drying overnight at 80 °C. The foil was then punched into 1 cm2 circular discs. PXRD was done using a Bruker D8-ADVANCE X-ray
diffractometer (Germany) with Cu Kα (wavelength = 1.5418 Å).
SEM was done using an FEI Nova Nano 450 SEM instrument.
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2

Characterization of Graphene on Cu Foil

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Tapping-mode AFM (Oxford Cypher VRS AFM system) was performed to characterize the surface morphology of the Cu foil before and after the electrochemical polishing pretreatment and the morphology and thickness of the graphene on SiO2/Si. The nucleation density, grain size, and morphology of the prepared graphene were characterized using optical microscopy (Axio scope A1) and scanning electron microscopy (SEM). The SEM images were acquired by using an FEI Nova Nano450-SEM instrument at 10 kV. The layer numbers and defects of the graphene were identified by Raman spectroscopy (Horiba Evolution) with a 532-nm laser.
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3

Multimodal Spectroscopic Analysis

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1H NMR, 13C NMR, and 19F NMR spectra were detected on a Bruker Biospin AG-400 (Bruker Optics, Ettlingen, Germany) or a JEOL-ECX500 (Akishima, Japan) apparatus with internal standard tetramethylsilane (TMS). High resolution mass spectrometry (HRMS) spectra were performed on a Q-Exactive Orbitrap MS apparatus (Thermo Fisher Scientific, Waltham, MA, USA). Scanning electron microscopy (SEM) images were obtained by using a Nova Nano SEM 450 instrument (FEI, USA, 200 kV). Fluorescent images were achieved under an Olympus-BX53 microscope (Olympus, Tokyo, Japan). ADDS-307 conductivity meter (INESA and Scientific Instrument Co., Ltd., Shanghai, China) was employed to record electrical conductivity. All of the chemicals (≥95%) used for reaction were purchased from Bide Pharmatech Ltd. (Shanghai, China).
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4

Electrochemical Biosensing of Biomarkers

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All nucleotide sequences used (Table 1 and Table S1, ordered from Sangon Biotech, Shanghai, China): tris (2-carboxyethyl) phosphine hydrochloride, named TCEP solution (Sigma-Aldrich, Shanghai, China); poly-HRP40 (streptavidin modified), named SA-polyHRP (Fitzgerald Industries International Inc., New Castle, DE, USA); TMB Substrate (Neogen, KY, USA); HAuCl4 (99.8% Au, Strem Chemicals Inc., Bischheim, France); fetal bovine serum, named FBS (Invitrogen, Carlsbad, CA, USA); all chemical buffer (Sangon Biotech, Shanghai, China). We also used: 16-mutichannel screen-printed carbon electrode, named 16-SPCE (CH Instruments, Inc., Shanghai, China); CHI-660C electrochemical workstation (CH Instruments, Inc., Shanghai, China); NanoDrop One (Thermo Fisher Scientific Inc., Tumwater, WA, USA); Nova nanoSEM 450 instrument (FEI Company, Rockville, MD, USA); and AFM Multimode 8 instrument (Bruker, Billerica, CA, USA).
Human serum samples were derived from healthy human blood, which was donated by volunteers. First, the whole blood was centrifuged at 3500 rpm for 10 min, then the supernatant was collected and kept at 4 °C for later use.
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5

Characterization of LJ-Wp, LJ-Gp, and LJ-Yp

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LJ-Wp, LJ-Gp, and LJ-Yp were analyzed by infrared spectroscopy using the potassium bromide tablet method, and FI-IR was analyzed by Vertex 70 FT-infrared spectroscopy in transmission mode. The wavelength range is 400–4000 cm1, and the resolution is 1 cm1.
The molecular weights of LJ-Wp, LJ-Gp, and LJ-Yp were determined by GPC-LS-IR, and the morphological characteristics of them were determined using an FEI Nova Nano SEM 450 instrument. The specific experimental method refer to Zhang et al. [43 (link)].
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