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Anti cd8α monoclonal antibody clone 2.43

Manufactured by BioXCell

The Anti-CD8α monoclonal antibody (Clone 2.43) is a laboratory reagent used for the identification and analysis of CD8-positive cells. It recognizes the CD8α chain, a glycoprotein expressed on the surface of cytotoxic T cells and a subset of natural killer cells. This antibody can be utilized in various immunological techniques, such as flow cytometry, to detect and quantify CD8-positive cell populations.

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2 protocols using anti cd8α monoclonal antibody clone 2.43

1

Rare-earth Nanoparticle Synthesis and Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rare-earth(III) acetate hydrate (RE: Yb, Er, Ce), zinc acetate, sodium trifluoroacetate, oleic acid (OA), 1-octadecene (ODE), sodium hydroxide, ammonium fluoride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Poly(acrylic acid) (PAA; average Mw 1800), poly(maleic anhydride-alt-1-octadecene) (PMH; 30–50 kDa), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), cyclohexane, chloroform, 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base), fetal bovine serum (FBS), phosphate buffered saline (PBS), and RPMI-1640 medium were purchased from Sigma-Aldrich and used without further purification. Yttrium(III) oxides and trifluoroacetic acid (99%) were purchased from Alfa Asea and used as received. Methoxy polyethylene glycol amine (mPEG-NH2; 5000 m.W.) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2; 40 kDa) was purchased from Advanced Biochemicals. Anti-PD-L1 monoclonal antibody (Atezolizumab, Clone SP142) was purchased from Selleckchem. Anti-CD8α monoclonal antibody (Clone 2.43) was purchased from Bio X Cell. EZ-Link™ Sulfo-NHS-LC-Biotin and DRAQ5 Fluorescent Probe were purchased from Thermo Fisher Scientific. Streptavidin was purchased from ProSpec. Y(CF3COO)3 was prepared by the literature method.51 Detailed information on the antibodies is included in the Life Sciences Reporting Summary.
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2

Rare-earth Nanoparticle Synthesis and Functionalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rare-earth(III) acetate hydrate (RE: Yb, Er, Ce), zinc acetate, sodium trifluoroacetate, oleic acid (OA), 1-octadecene (ODE), sodium hydroxide, ammonium fluoride, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Poly(acrylic acid) (PAA; average Mw 1800), poly(maleic anhydride-alt-1-octadecene) (PMH; 30–50 kDa), 4-morpholineethanesulfonic acid (MES), 4-(dimethylamino)pyridine (DMAP), cyclohexane, chloroform, 2-Amino-2-(hydroxymethyl)-1,3-propanediol (tris-base), fetal bovine serum (FBS), phosphate buffered saline (PBS), and RPMI-1640 medium were purchased from Sigma-Aldrich and used without further purification. Yttrium(III) oxides and trifluoroacetic acid (99%) were purchased from Alfa Asea and used as received. Methoxy polyethylene glycol amine (mPEG-NH2; 5000 m.W.) was purchased from Laysan-Bio. 8-arm polyethylene glycol amine (8Arm-PEG-NH2; 40 kDa) was purchased from Advanced Biochemicals. Anti-PD-L1 monoclonal antibody (Atezolizumab, Clone SP142) was purchased from Selleckchem. Anti-CD8α monoclonal antibody (Clone 2.43) was purchased from Bio X Cell. EZ-Link™ Sulfo-NHS-LC-Biotin and DRAQ5 Fluorescent Probe were purchased from Thermo Fisher Scientific. Streptavidin was purchased from ProSpec. Y(CF3COO)3 was prepared by the literature method.51 Detailed information on the antibodies is included in the Life Sciences Reporting Summary.
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