The ethanol extract of Pueraria lobatae Radix (EPL, Hanzhong Trg Biotech Co., Ltd., Shanxi, China), and ammonium bicarbonate (NH4HCO3, SinopharmChemical Reagent Co., Shanghai, China) were used as supplied.
Nh4hco3
Manufactured by Sinopharm
Sourced in China
NH4HCO3 is a chemical compound that is commonly used in various industrial and laboratory applications. It is a white, crystalline solid with a mildly alkaline taste. The compound's core function is to serve as a pH regulator and buffer in various chemical processes.
Automatically generated - may contain errors
Lab products found in correlation
Mgcl2 6h2o, by Sinopharm (2 mentions)
Ethanol, by Sinopharm (1 mentions)
Fecl3 6h2o, by Sinopharm (1 mentions)
Nah2po4 2h2o, by Sinopharm (1 mentions)
Cacl2 2h2o, by Sinopharm (1 mentions)
Tert butyl alcohol, by Sinopharm (1 mentions)
H2so4, by Sinopharm (1 mentions)
Na2b4o7 10h2o, by Sinopharm (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Potassium chloride (kcl), by Sinopharm (1 mentions)
Urea, by Sinopharm (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Sodium chloride (nacl), by Sinopharm (1 mentions)
Na2so4, by Sinopharm (1 mentions)
Acetonitrile, by CNW Technologies (1 mentions)
Methanol, by CNW Technologies (1 mentions)
Nahco3, by Sinopharm (1 mentions)
H3bo3, by Sinopharm (1 mentions)
Na2s2o3, by Sinopharm (1 mentions)
Nh4oh, by Sinopharm (1 mentions)
6 protocols using nh4hco3
1
Ethanol Extract of Pueraria Lobatae
2
Synthesis of MWCNTs-based Materials
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FeCl3·6H2O, NH4HCO3, and ethanol were purchased
from Sinopharm Chemical Reagent Co., Ltd. (Ourchem Shanghai). MWCNTs
were supplied by Timesnano China (Chengdu Organic Chemicals Co. Ltd.,
Chinese Academy of Sciences). All chemicals employed in the present
paper were analytical reagents and used as received. Carbon paper
(CeTech, Taiwan) was employed as the conductive substrate for the
as-synthesized materials. Deionized (DI) water with a resistivity
of over 18.25 MΩ·cm at 23 °C was used throughout the
experiment.
from Sinopharm Chemical Reagent Co., Ltd. (Ourchem Shanghai). MWCNTs
were supplied by Timesnano China (Chengdu Organic Chemicals Co. Ltd.,
Chinese Academy of Sciences). All chemicals employed in the present
paper were analytical reagents and used as received. Carbon paper
(CeTech, Taiwan) was employed as the conductive substrate for the
as-synthesized materials. Deionized (DI) water with a resistivity
of over 18.25 MΩ·cm at 23 °C was used throughout the
experiment.
3
Analytical Techniques for Biochemical Compounds
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All the chemical reagents used in the experiment were of analytical grade or higher purity. ACE, ethanol (EtOH), tert-butyl alcohol (TBA), ascorbic acid (AA), Na3 citrate·2H2O, NaOH, urea, NaCl, KCl, NH4OH, NaH2PO4·2H2O, MgCl2·6H2O, CaCl2·2H2O, NH4HCO3, NaHCO3, H2SO4, Na2S2O3, Na2SO4, H3BO3, Na2B4O7·10H2O, and KI were obtained from Sinopharm (China). Creatine, creatinine, hippuric acid, nitrobenzene (NB), furfuryl alcohol (FFA), nitro blue tetrazolium (NBT), l -histidine (l -his), and trifluoroacetic acid were supplied by Aladdin Chemicals. Methanol and acetonitrile were purchased from CNW Technologies GmbH (Germany). The preparation of the matrices involved dissolution in ultrapure water (18.2 MΩ cm−1).
4
Mouse Liver Tissue Proteomics Protocol
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The liver tissue from each mouse was manually homogenized into powder in liquid nitrogen using a mortar and pestle. Samples with equal weight tissue (200 mg) from each group were pooled into three fractions. The powder was mixed with lysis buffer (8 M urea (Sangon Biotech Co., Ltd., Shanghai, China), 2 M thiourea (Sangon Biotech Co., Ltd., Shanghai, China), 4% CHAPS (Sangon Biotech Co., Ltd., Shanghai, China), 20 mM Tris-base (Sangon Biotech Co., Ltd., Shanghai, China), 30 mM dithiothreitol (Sangon Biotech Co., Ltd., Shanghai, China)) and periodically vortexed in ice for 30 min and then centrifuged at 10,000× g for 15 min at 4 °C. Then protein pellets were dissolved with 100 µL of 5 M urea and then mixed with four volumes of 40 mM NH4HCO3 (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) solution. The protein concentration was determined using a bicinchoninic acid (BCA) (Beyotime Biotechnology, Shanghai, China) assay. The protein samples were reduced with dithiothreitol solution (final concentration 10 mM) and then alkalized with iodoacetamide (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China) solution (final concentration: 50 mM). Subsequently, protein samples were digested with sequencing-grade modified trypsin (Beyotime Biotechnology, Shanghai, China) at 37 °C overnight (protein:trypsin = 40:1) [18 (link)].
5
Oridonin-loaded PLGA Nanoparticles
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Oridonin was obtained from Shaanxi Huike
Botanical Development Co., Ltd. (Shaanxi, China). PLGA [poly(d ,l -lactic-co-glycolic)acid, lactide/glycolide,
75:25, mol/mol, MW, 10 kDa] was obtained from Jinan Daigang Biomaterial
Co., Ltd. (Shandong, China). Gemcitabine was used as a positive drug
and purchased from Hansoh Pharmaceutical Co., Ltd. (Jiangsu, China).
NH4HCO3 was purchased from Sinopharm Chemical
Reagent Co., Ltd. (Beijing, China). Cy7 was purchased from Fanbo Biochemicals
Co., Ltd. (Beijing, China). All other chemicals and solvents were
of analytical grade or high-performance liquid chromatographic (HPLC)
grade.
Botanical Development Co., Ltd. (Shaanxi, China). PLGA [poly(
75:25, mol/mol, MW, 10 kDa] was obtained from Jinan Daigang Biomaterial
Co., Ltd. (Shandong, China). Gemcitabine was used as a positive drug
and purchased from Hansoh Pharmaceutical Co., Ltd. (Jiangsu, China).
NH4HCO3 was purchased from Sinopharm Chemical
Reagent Co., Ltd. (Beijing, China). Cy7 was purchased from Fanbo Biochemicals
Co., Ltd. (Beijing, China). All other chemicals and solvents were
of analytical grade or high-performance liquid chromatographic (HPLC)
grade.
6
Analytical-grade Chemical Reagents Protocol
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Chemical reagents,
MgCl2·6H2O, NH4HCO3, aqueous NH3·H2O, SDS, toluene, and acridine
orange were analytical grade and purchased from Sinopharm Chemical
Reagent Co., Ltd., China. All chemicals were used directly in the
experiments without further treatment. The water used in all experimental
work for solution preparation, dilution, washing, and so forth was
99.9% double distilled water (conductivity < 0.1 μS·cm–1).
MgCl2·6H2O, NH4HCO3, aqueous NH3·H2O, SDS, toluene, and acridine
orange were analytical grade and purchased from Sinopharm Chemical
Reagent Co., Ltd., China. All chemicals were used directly in the
experiments without further treatment. The water used in all experimental
work for solution preparation, dilution, washing, and so forth was
99.9% double distilled water (conductivity < 0.1 μS·cm–1).
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