Rabbit anti acetyl lysine

Manufactured by Cell Signaling Technology

Rabbit anti-acetyl-lysine is a primary antibody that specifically recognizes proteins containing acetylated lysine residues. It can be used to detect and quantify acetylated proteins in various experimental techniques.

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Anti-acetyl lysine (40 citations) Acetyl-lysine (35 citations) Rabbit anti-acetyl lysine antibody (6 citations) Rabbit anti Acetyl-lysine antibody (9441S) (2 citations)

3 protocols using rabbit anti acetyl lysine

Antibody Characterization for Tau Research

Cited 2 times

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The rabbit anti-ac-KIGS antibody (ac-Lys-259/353) was generated and affinity-purified by 21st Century Biochemicals (Marlboro, MA), as described (7 (link)). E1 (human-specific tau antibody, rabbit host) was generated by our group against amino acid residues 19–33 within exon 1 of human tau (15 (link), 35 (link), 36 (link)). Mouse monoclonal PHF1 (pSer-396/404) and CP13 (pSer-202) antibodies were provided by P. Davies (Feinstein Institute for Medical Research, Northwell Health). Mouse monoclonal 12E8 (anti-pSer-262/356) was provided by P. Seubert (previously at Elan Pharmaceuticals, San Francisco, CA). In addition, we purchased rabbit anti-acetyl-lysine (catalogue no. 9441S, lots 11 and 12) from Cell Signaling Technology, Inc. (Danvers, MA), rabbit anti-pSer-324 (catalogue no. GTX62906, lots 821404449 and 8214010622) from GeneTex (Irvine, CA), and mouse anti-GAPDH (catalogue no. H86504M, lot 181.34316) from Meridian Life Science, Inc. (Memphis, TN). Secondary antibodies were obtained from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA).

Carlomagno Y., Chung D.E., Yue M., Castanedes-Casey M., Madden B.J., Dunmore J., Tong J., DeTure M., Dickson D.W., Petrucelli L, & Cook C. (2017). An acetylation–phosphorylation switch that regulates tau aggregation propensity and function. The Journal of Biological Chemistry, 292(37), 15277-15286.

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Western Blot Analysis of Cellular Proteins

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Cells were lysed and extracts prepared as described (24). Primary antibodies included: rabbit anti-PAI-1 (1:3000) [25 (link)], rabbit anti-p53 (1:2000; Cell Signaling), rabbit anti-ERK2 (1:5000; Santa Cruz); rabbit anti-phospho-SMAD2/3 (1:500; Santa Cruz), rabbit anti-β-actin (1:5000; Santa Cruz), mouse anti-Fibronectin (1:2000; BD Biosciences), rabbit anti-CTGF/CCN2 (1:2000, Abcam), mouse anti-α-smooth muscle actin (1:2000; BD Biosciences), rabbit anti-COX-2 (1:2000, Santa Cruz), rabbit anti-p21 (1:1000, Santa Cruz), rabbit anti-phospho-p53 sampler kit (1:1000; Cell Signaling), rabbit anti-phospho-p53^Ser15 (1:1000; Cell Signaling), rabbit anti-phospho-SMAD3 (1:2000, Thermoscientfic), rabbit anti-lamin A C (1:1000, Santa Cruz), rabbit anti-SMAD3 (1:2000, Abcam), rabbit anti-SMAD2 (1:1000, Santa Cruz), mouse anti-GFP (1:2000, Santa Cruz), rabbit anti-acetyl-lysine (1:1000, Cell Signaling), rabbit anti-p300 (1:2000, Santa Cruz).

Overstreet J.M., Samarakoon R., Meldrum K.K, & Higgins P.J. (2014). Redox control of p53 in the transcriptional regulation of TGF-β1 target genes through SMAD cooperativity. Cellular signalling, 26(7), 1427-1436.

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Protein Interactions in Cellular Signaling

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Lysates from the ipsilateral hemisphere were immunoprecipitated overnight at 4 °C with mouse anti-p65 (1:100, Cell Signaling Technology)/normal IgG (1:100, Sigma-Aldrich), mouse anti-p53 (1:50, Santa Cruz Biotechnology)/normal IgG (1:50, Sigma-Aldrich), and then conjugated to nProtein A Sepharose^TM 4 Fast Flow/Protein G Sepharose^TM 4 Fast Flow (GE Healthcare Bio-Science AB, Uppsala, Sweden) for 2 h at 4 °C. The complexes were washed three times with lysis buffer, denatured and subjected to Western blotting using rabbit anti-acetyl-lysine (1:1000, Cell Signaling Technology), mouse anti-p53 (1:500, Santa Cruz Biotechnology), rabbit anti-NF-κB p65 (acetyl K310) (1:3000, Abcam), and mouse anti-p65 (1:1000, Cell Signaling Technology).

Li M., Li S.C., Dou B.K., Zou Y.X., Han H.Z., Liu D.X., Ke Z.J, & Wang Z.F. (2020). Cycloastragenol upregulates SIRT1 expression, attenuates apoptosis and suppresses neuroinflammation after brain ischemia. Acta Pharmacologica Sinica, 41(8), 1025-1032.

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