Hela cell line

Manufactured by Merck Group

Sourced in United States

The HeLa cell line is a widely used human cell line derived from cervical cancer cells. It is one of the most commonly used cell lines in medical research and has been essential for numerous scientific advancements. The core function of the HeLa cell line is to provide a reliable and consistent source of human cells for various experimental purposes, such as studying cellular processes, testing new drugs, and advancing our understanding of cancer biology.

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Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Pen strep, by Thermo Fisher Scientific (2 mentions) Ab203826, by Abcam (1 mentions) Ab108312, by Abcam (1 mentions) Sc 6260, by Abcam (1 mentions) Chemidoctm xrs system, by Bio-Rad (1 mentions) Methylene blue, by Merck Group (1 mentions) Kolliphor p188, by Merck Group (1 mentions) Dmso d6, by Eurisotop (1 mentions) Synperonic pe p84, by Merck Group (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Na2hpo4, by Merck Group (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Fetal bovine serum (fbs), by Biowest (1 mentions) Hrv 2, by European Collection of Authenticated Cell Cultures (1 mentions) Emem medium, by Thermo Fisher Scientific (1 mentions) Countess, by Thermo Fisher Scientific (1 mentions) Vorinostat, by Merck Group (1 mentions)

12 protocols using hela cell line

Characterization of Limbal Stem Cell Markers

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Using Western blot (WB), the expression of typical LSC markers ATP-binding cassette superfamily G member 2 (ABCG2), p63, anti-p40-deltaNp63, and vimentin was measured as described in the literature [4 (link), 11 (link), 12 (link)]. First, cellular proteins from cLSCs were extracted using RIPA buffer with protease inhibitors. This extract was kept on ice for 30 minutes, and the lysis mixture was centrifuged at 11,000 g and 4°C for 15 minutes. The supernatant was transferred to a new tube, and the protein concentration was determined using the bicinchoninic acid kit (BCA, SERVA).
For WB, 30 μg of protein was used, and the membranes were incubated with mouse anti-ABCG2 (Abcam, ab108312), mouse anti-p63 (Abcam, ab124762), anti-vimentin (Santa Cruz Biotechnology, sc-6260) and rabbit anti-deltaNp63 (Abcam, ab203826) at 1:1000 dilution. As a positive control for ABCG2, p63 and deltaNp63, a protein lysate from the HeLa cell line (Merck) was used, and in the case of vimentin, a protein lysate of human MSC from adipose tissue (hAd-MSC) was used as a positive control. Secondary anti-mouse (Merck) and anti-rabbit (Abcam) antibodies were used for detection by ECL (SERVA), and the results were visualized in the ChemiDocTM XRS + system (Bio-Rad).

Villatoro A.J., Alcoholado C., Martín-Astorga M.D., Rico G., Fernández V, & Becerra J. (2020). Characterization of the secretory profile and exosomes of limbal stem cells in the canine species. PLoS ONE, 15(12), e0244327.

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Synthesis and Characterization of Pc1 Nanoformulations

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1(4), 8(11), 15(18), 22(25)-(2-(2-(2-methoxyethoxy)ethoxy)ethoxy)-Zn(II)
phthalocyanine (Pc1, MW = 1226.64 g/mol) was synthesized
as previously described.^{27 (link),32 (link)} PVP (average MW = 10
kDa), PVP (average MW = 40 kDa), PEG–PPG–PEG triblock
copolymers Kolliphor P188 (P188, average MW = 8.4 kDa), Synperonic
PE/P84 (P84, average MW = 4.2 kDa), and Pluronic F-127 (F127, average
MW = 12.6 kDa), the PEG ester of hydrogenated castor oil Kolliphor
RH 40 (RH40, average MW = 2.5 kDa), and methylene blue (MB) were purchased
from Sigma-Aldrich. Deuterated solvents, DMSO-d₆ (99.95%) and D₂O (99.9%), used for the NMR measurements
were purchased from Eurisotop (St.-Aubin Cedex, France) and Deutero
GmbH (Kastelaun, Germany), respectively. Phosphate-buffered saline
[(PBS), 50 mM, pH = 7.3] was prepared by mixing aliquots of 50 mM
solutions of KH₂PO₄ and Na₂HPO₄ (provided by Sigma-Aldrich) in D₂O or H₂O containing 0.9% NaCl. The human epithelioid cervix carcinoma HeLa
cell line was purchased from Merck and cultured in minimum essential
medium (MEM, Gibco). Paraformaldehyde [(PFA), 16%, Electron Microscopy
Sciences] was used for cell fixation.

Gergely L.P., Yüceel Ç., İşci Ü., Spadin F.S., Schneider L., Spingler B., Frenz M., Dumoulin F, & Vermathen M. (2023). Comparing PVP and Polymeric Micellar Formulations of a PEGylated Photosensitizing Phthalocyanine by NMR and Optical Techniques. Molecular Pharmaceutics, 20(8), 4165-4183.

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HeLa Cell Culture Protocol

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The HeLa cell line used in this study was purchased from Sigma Aldrich (Saint Louis, MO, USA) and cells were cultured in DMEM medium containing 10% fetal bovine serum, 2 mM glutamine GlutaMAX, 100 units/ml penicillin, and 100 µg/ml streptomycin at 37 °C in a humidified atmosphere of 5% CO₂. HeLa EFR3A-knockdown (KnD) and HeLa “scrambled” control were cultured in the same medium additionally supplemented with 2 µg/ml puromycin at 37 °C in a humidified atmosphere of 5% CO₂.

Trybus M., Hryniewicz-Jankowska A., Wójtowicz K., Trombik T., Czogalla A, & Sikorski A.F. (2023). EFR3A: a new raft domain organizing protein?. Cellular & Molecular Biology Letters, 28, 86.

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Culturing HeLa Cells in DMEM

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HeLa cell line (93021013 Sigma-Aldrich) was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with 10% fetal bovine serum (Biowest SAS, France), 4 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin solution. Cells were cultured in a humidified atmosphere containing 5% CO₂ at 37 °C.

Sztilkovics M., Gerecsei T., Peter B., Saftics A., Kurunczi S., Szekacs I., Szabo B, & Horvath R. (2020). Single-cell adhesion force kinetics of cell populations from combined label-free optical biosensor and robotic fluidic force microscopy. Scientific Reports, 10, 61.

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In vitro HeLa Cell Culture

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In vitro assays were performed with human cervical cancer (HeLa) cell line (Sigma-Aldrich).
Cells were cultured up to 4 days in 48-well plates at a density of 5000 cells/sample and maintained in complete high glucose Dulbecco modified Eagle’s medium (DMEM, Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher Scientific) and 1% Penicillin and Streptomycin (PenStrep, Thermo Fisher Scientific).

Parisi L., Toffoli A., Bianchi M.G., Bergonzi C., Bianchera A., Bettini R., Elviri L, & Macaluso G.M. (2019). Functional Fibronectin Adsorption on Aptamer-Doped Chitosan Modulates Cell Morphology by Integrin-Mediated Pathway. Materials, 12(5), 812.

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Inactivation and Infection of Human Rhinoviruses

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Two serotypes of human rhinoviruses (HRV) 16 and HRV‐2 were purchased from the European Collection of Authenticated Cell Cultures (ECACC)). Ohio HeLa cell line, purchased from Sigma‐Aldrich was infected until cytopathic effect was observed multiplicity of infection (MOI) of 1, established on the base of literature.²², ²³ HRV specimens were exposed to the temperature of 58°C for 1 h in order to inactivate the virus particles,²⁴ which was subsequently confirmed by a lack of HRV replication.
The target fibroblast and epithelial cells were infected by the addition of 50 μL vehicle (medium) or HRV16. The cells were incubated for 24 h (33°C, 5% CO₂).²⁵

Wieczfinska J, & Pawliczak R. (2023). Anti‐fibrotic effect of ciglitazone in HRV‐induced airway remodelling cell model. Journal of Cellular and Molecular Medicine, 27(13), 1867-1879.

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Evaluating MYBPC3 Variant c.3331-26T>G

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The consequences of the MYBPC3 variant c.3331−26T>G on pre-mRNA splicing were evaluated, as previously described⁴⁶, by RT-PCR and Western blot analysis of HeLa cells transiently transfected with the reference 1446 and mutant 1447 MYBPC3 plasmids. The HeLa cell line was purchased from the European Collection of Authenticated Cell Cultures (Sigma-Aldrich) and maintained in the cell culture medium recommended by the manufacturer. Primary stocks propagated from the original frozen culture were used (passage < + 8). The day before transfection, cells were trypsinized from culture flasks and plated in 12-well culture plates, allowed to attach overnight and transiently transfected with 1.0 µg of plasmid DNA. Cellular transfections with each plasmid were performed in triplicate wells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions. Transfection experiments were repeated twice and cells were harvested 48 h after transfection, processing each cell culture well independently for RNA or protein extraction.

Torrado M., Maneiro E., Lamounier Junior A., Fernández-Burriel M., Sánchez Giralt S., Martínez-Carapeto A., Cazón L., Santiago E., Ochoa J.P., McKenna W.J., Santomé L, & Monserrat L. (2022). Identification of an elusive spliceogenic MYBPC3 variant in an otherwise genotype-negative hypertrophic cardiomyopathy pedigree. Scientific Reports, 12, 7284.

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HeLa Cell Culture Protocol

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Hela cell line was purchased from Sigma Chemical Company (St Louis). The cells were seeded in 12-well plates containing E’MEM medium (GIBCO, serum-free medium) supplemented with L-glutamine (200 mM), HEPES (1 M), amphotericin B (0.1%), penicillin-streptomycin 200 x and 10% (v/v) FBS. The cells were cultured under a humidified 5% CO₂; at 37°C to achieve ~90% confluency was used in the photodynamic and laser treatment.

Bui H.T., Pham T.T., Nguyen H.T., Do T.M., Nga V.T., Bac N.D., Huyen V.T., Le H.M, & Tran Q.C. (2019). Transformation Chlorophyll a of Spirulina platensis to Chlorin e6 Derivatives and Several Applications. Open Access Macedonian Journal of Medical Sciences, 7(24), 4372-4377.

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Evaluating KCNQ1 Variant Splicing Effects

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The consequences of the KCNQ1 variant c.1686−9 T > C on RNA splicing were evaluated by RT-PCR and western blot analysis of HeLa cells transiently transfected with the REF or MUT minigene plasmids, as previously described³². The HeLa cell line was purchased from the European Collection of Authenticated Cell Cultures (Sigma–Aldrich) and cultured in the cell culture medium recommended by the manufacturer. Cells were trypsinized at 80% confluence, and cell numbers were determined using an automated cell counter (Countess, Invitrogen) and plated in 12-well culture plates, allowed to attach overnight and transiently transfected with 1.0 µg of plasmid DNA. Cellular transfections with REF or MUT plasmids were performed in triplicated wells using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions. The cells were harvested 48 h after transfection. Each cell culture well was processed independently for RNA or protein extraction, and transfection experiments were repeated twice in each assay. All gels and blots in Figures contained a set of samples processed and analyzed in parallel.

Torrado M., Fernández G., Ganoza C.A., Maneiro E., García D., Sonicheva-Paterson N., Rosa I., Ochoa J.P., Santomé L., Vasichkina E, & Monserrat L. (2021). A cryptic splice-altering KCNQ1 variant in trans with R259L leading to Jervell and Lange-Nielsen syndrome. NPJ Genomic Medicine, 6, 21.

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Cytotoxicity of HDAC inhibitors in astrocyte and HeLa cells

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The SVG astrocyte cell line [18] (link) was cultured in Minimum Essential Medium (MEM) with Earle's salts supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS), 1× penicillin-streptomycin-glutamine (PSG) (Invitrogen, Carlsbad, CA). The HeLa cell line (ATCC) was cultured in MEM containing 10% HI-FBS and 1× PSG.
Each cell line was treated with varying concentrations of trichostatin A (TSA; Sigma), vorinostat [suberoylhydroxamic acid (SAHA)], panobinostat (LBH589) and entinostat (MS-275; all from Selleck Chemicals, Houston, TX) for 24 hours and toxicity was assessed with the MTS colorimetric assay according to manufacturer's instructions (Promega, Madison, WI). Experiments were performed in triplicate and the cytotoxic concentration 50 (CC₅₀) values were generated using GraphPad Prism software (Version 6, Graphpad, La Jolla, CA).

Lu H.K., Gray L.R., Wightman F., Ellenberg P., Khoury G., Cheng W.J., Mota T.M., Wesselingh S., Gorry P.R., Cameron P.U., Churchill M.J, & Lewin S.R. (2014). Ex Vivo Response to Histone Deacetylase (HDAC) Inhibitors of the HIV Long Terminal Repeat (LTR) Derived from HIV-Infected Patients on Antiretroviral Therapy. PLoS ONE, 9(11), e113341.

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