12 pahsa

Manufactured by Cayman Chemical

Sourced in United States

12-PAHSA is a chemical compound used in laboratory research. It is a fatty acid derivative that can be utilized for various analytical and experimental purposes. The core function of 12-PAHSA is to serve as a research tool, without further interpretation of its intended use.

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3 protocols using 12 pahsa

Comprehensive FAHFA Standards Acquisition

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The FAHFA standards, including 5-PAHSA, 9-PAHSA, 10-PAHSA, 12-PAHSA, 13-PAHSA, 5-SAHPA, 10-SAHPA, 5-MAHMA, 9-MAHMA, 10-MAHMA, 5-MAHPA, 9-MAHPA, 10-MAHPA, 5-PAHMA, 9-PAHMA, 10-PAHMA, 5-PDAHPA, 10-PDAHPA, 5-PAHPA, 9-PAHPA, 10-PAHPA, 5-SAHMA, 10-SAHMA, 7-PDAHSA, 9-PDAHSA, 10-PDAHSA, 10-POHSA, 5-POHSA, 12-POHSA, 13-POHSA, 5-OAHSA, 9-OAHSA, 12-OAHSA, 13-OAHSA, 5-SAHSA, 10-SAHSA, 12-SAHSA, 13-SAHSA, and 10-OSHSA were purchased from Cayman Chemical (Ann Arbor, MI, USA), RC ChemTecCo., Ltd. (Wuhan, Hubei, China), and XuKang Medical Science and Technology Co., Ltd. (Xiangtan, Hunan, China). 2-Chloro-1-methylpyridinium iodide (CMPI), triethylamine (TEA), and 2-dimethylaminoe-thylamine (DMED) were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
HPLC grade acetonitrile (ACN), acetone, chloroform, and methanol were purchased from Merck (Darmstadt, Germany). Analytical grade formic acid, and ammonium hydroxide were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Strong anion-exchange solid-phase extraction cartridges (3 mL, 200 mg) were supplied by Weltech Co. (Wuhan, China).

Ding J., Kind T., Zhu Q.F., Wang Y., Yan J.W., Fiehn O, & Feng Y.Q. (2020). In-Silico-Generated Library for Sensitive Detection of 2-Dimethylaminoethylamine Derivatized FAHFA Lipids Using High-Resolution Tandem Mass Spectrometry. Analytical chemistry, 92(8), 5960-5968.

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FAHFA Biosynthesis and Bioassays

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The following FAHFAs were obtained from Cayman Chemical (Ann Harbor, MI): 5-PAHSA (Cat# 17043), 9-PAHSA (Cat# 17037), 10-PAHSA (Cat# 19973), 12-PAHSA (Cat# 17107), 13-PAHSA (Cat# 17044), 5-POHSA (Cat# 17114), 9-POHSA (Cat# 17040), 10-POHSA (Cat# 19974), 12-POHSA (Cat# 17105), 13-POHSA (Cat# 17111), 5-OAHSA (Cat# 17115), 9-OAHSA (Cat# 17039), 10-OAHSA (Cat# 19975), 12-OAHSA (Cat# 17108), 13-OAHSA (Cat# 17112), 5-SAHSA (Cat# 17113), 9-SAHSA (Cat# 17109), 10-SAHSA (Cat# 19976), 12-SAHSA (Cat# 17106), and 13-SAHSA (Cat# 17110). R and S enantiomers of 9-PAHSA were synthesized as previously described (25 (link)). Concentrations of FAHFAs in all biological assays is 20 μM, concentration of DMSO is 0.01% unless mentioned otherwise. The Human Insulin ELISA kit (Cat#80-INSHU-E01.1) was purchased from ALPCO (Salem, NH). The Mouse Insulin ELISA kit (Cat# 90080) was obtained from Crystal Chem (Elk Grove Village, IL). Mouse Tnfα (Cat# 430901) and Il-6 (Cat# 431301) ELISA kits were obtained from BioLegend (San Diego, CA). [3H]Deoxy-glucose (NET328A) was obtained from PerkinElmer (Waltham, MA). All the other chemicals were purchased from Sigma-Aldrich (St Louis, MO) unless otherwise stated.

Aryal P., Syed I., Lee J., Patel R., Nelson A.T., Siegel D., Saghatelian A, & Kahn B.B. (2021). Distinct biological activities of isomers from several families of branched fatty acid esters of hydroxy fatty acids (FAHFAs). Journal of Lipid Research, 62, 100108.

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Quantitative Analysis of Metabolites

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The standard stock solutions of 5-PAHSA, 9-PAHSA, 12-PAHSA, 9-PAHPA, 9-SAHSA, 9-POHSA, 9-OAHSA, PA, SA, POA, and OA were purchased from Cayman Chemical (Ann Arbor, MI, USA). The standards of l-Hcy, SAH, l-Car, and TMAO (Sigma Aldrich, St. Louis, MO, USA) were dissolved in double-distilled water (ddH₂O) or HPLC grade methanol (MeOH) with final concentration 0.1 mg/mL for stock solution. The stable isotopes ¹³C₁₆-palmitic acid (¹³C₁₆-PA) and d3, l-methionine (d3-l-Met) were obtained from Sigma Aldrich (St. Louis, MO, USA), and the d9-TMAO was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). All of the stock solutions and stable isotopes solution were stored at −20 °C and the storage time was less than one year. The calibration curves were prepared by using stock dilution and formulated by using the peak area ratio of the analytical standards and the internal standards.

Dongoran R.A., Lin T.J., Byekyet A., Tang S.C., Yang J.H, & Liu C.H. (2020). Determination of Major Endogenous FAHFAs in Healthy Human Circulation: The Correlations with Several Circulating Cardiovascular-Related Biomarkers and Anti-Inflammatory Effects on RAW 264.7 Cells. Biomolecules, 10(12), 1689.

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