C57BL/6J (B6), B6.129P2-B2mtm1Unc/J (β2m−/−), B6.SJL-PtprcaPepcb/BoyJ (B6 CD45.1), B6.129P2-Ptpn6tm1Rsky/J (Ptpn6fl/+), and NOD.Cg-Rag1tm1MomIl2rgtm1Wjl/SzJ (NRG) mice were obtained from The Jackson Laboratory. NOD.Cg-Rag1tm1MomIL2rgtmWjl Tg (B2M, HLA-B*27–05)56–3 (B27 Tg+) mice were described previously (39 (link)). NKp46-CreERT2 Tg mice carrying the Rosa26-td-Tomato allele (40 (link)) were kindly provided by Dr. L. Lanier (University of California), courtesy of Dr. J. Sun (MSKCC). Where indicated in the figure legends, mice were administered 4 mg of tamoxifen (Sigma) by oral gavage in 200 μl of corn oil (Sigma). All of the mice used in this study were housed and bred under specific pathogen-free conditions at Memorial Sloan Kettering Cancer Center and handled in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC).
Nod cg rag1tm1momil2rgtm1wjl szj nrg
Manufactured by Jackson ImmunoResearch
The NOD.Cg-Rag1tm1MomIl2rgtm1Wjl/SzJ (NRG) mouse is a laboratory animal model. It is a double mutant strain that lacks functional genes for the recombination-activating gene 1 (Rag1) and the interleukin-2 receptor gamma chain (Il2rg). These genetic modifications result in the absence of mature T cells and B cells, as well as impaired natural killer cell function.
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2 protocols using nod cg rag1tm1momil2rgtm1wjl szj nrg
1
Transgenic Mouse Strains for Immunology
2
Bioluminescent Imaging of Melanoma Xenografts
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Male NOD.Cg-Rag1tm1Mom Il2rgtm1Wjl/SzJ (NRG, The Jackson Laboratory, Bar Harbor, ME) mice aged six to eight weeks were anesthetized with a solution of ketamine (9.5 mg/ml; 99/192/85-C, Bioveta, Ivanovice na Hané, Czech Republic) and xylazine (0.95 mg/ml; A6A066, Bioveta, Ivanovice na Hané, Czech Republic) at a final dose of 100 µl/10 g of body weight, administered intraperitoneally. The mice were injected intradermally with 5 × 104 A375 IV GFP Luc2 cells in 50 µl PBS. Live cell imaging was performed using the IVIS Lumina XR optical imaging system (Perkin Elmer, Waltham, MA) 15 min after intraperitoneal injection of D-luciferin, sodium salt (LUCNA-10G, Goldbio, St. Louis, MO, 150 mg/kg) with exposure times of 15 s, 30 s, and 60 s. Luminescence (total flux [p/s]) was quantified using Living Image v4.7.2 software (Perkin Elmer, Waltham, MA). All experiments were conducted with the approval of the Academy of Sciences of the Czech Republic (AVCR 103/2019), overseen by the ethical committee of the Institute of Biophysics CAS, performed by certified individuals (MP, RV), and carried out in accordance with relevant guidelines and regulations. All methods are reported in accordance with ARRIVE guidelines.
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