Three‐day‐old flies were collected and frozen at −80°C. When taking pictures, flies were unfrozen at room temperature and placed on 1% agarose plate. Light images of eye were taken by OLYMPUS stereo microscope SZX16 (Olympus Corporation, Shinjuku, Tokyo, Japan). Wings were dissected and placed on slide with alcohol/glycerol (1:1) buffer. Light images of wing were taken by OLYMPUS BX51 microscope.
Stereo microscope szx16
Manufactured by Olympus
Sourced in Japan
The Olympus SZX16 is a stereo microscope designed for versatile laboratory use. It provides a wide field of view and high-resolution imaging capabilities to facilitate detailed observation and analysis of samples.
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Lab products found in correlation
7 protocols using stereo microscope szx16
1
Imaging Fly Eyes and Wings
2
Visualizing Drosophila Eye and Wing
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Images of fly eyes and wings were prepared as described [69 (link)]. Briefly, 3-day-old flies were collected and frozen at − 80 °C for more than 12 h. When taking pictures, flies were unfrozen at room temperature and placed on 1% agarose plate. Light images of eye were taken by OLYMPUS stereo microscope SZX16 (Olympus Corporation, Shinjuku, Tokyo, Japan). Wings were dissected and placed on slide with alcohol/glycerol (1:1) medium. Light images of wing were taken by OLYMPUS BX51 microscope. Adobe Photoshop 2014 was used to measure the size of fly wings and eyes on the images.
3
Characterizing Drosophila Morphology
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Three-day-old flies were collected and frozen at − 80 °C. When taking pictures, flies were unfrozen at room temperature and placed on 1% agarose plate. Light images of eyes were taken by OLYMPUS stereo microscope SZX16 (Olympus Corporation, Shinjuku, Tokyo, Japan). Light images of wings, dissected and placed on slide with alcohol/glycerol (1:1) medium, were taken by OLYMPUS BX51 microscope.
4
Ovarian Development and Fecundity Assay in Bactrocera dorsalis
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Different treated female B. dorsalis were anesthetized on ice and dissected in cold PBS to evaluate the ovary developmental stage on days 7, 10 and 13 post eclosion, with at least 30 flies for each treatment. Ovarian morphology was observed and imaged using an Olympus Stereo Microscope SZX16 (Olympus, Tokyo). The ovarian morphology was divided into five stages as prior literature [41 (link)], including previtellogenic stage (I), vitellogenic deposition stage (II), expectant stage of mature eggs (III), peak stage of oviposition (IV), and last stage of oviposition (IV). The ovarian proportions were the ratio of the number of ovaries in corresponding stages to the number of all ovaries (n = 30). For the fecundity assay, different treated females were allowed to culture until sexual mature (12-day-old), and mated with virgin males of the same age in a self-developed egg counting device. Each group contained two couple of flies and ten replicates were performed. The number of laid eggs in each cup was counted at 1, 3, 5, 7, 9, 11, 13, and 15 days after mating, and the total number of laid eggs was calculated. To measure the hatching rate, about 200 embryos were collected and maintained on wet filter paper, and hatched larvae were counted 2 days later after hatching. Mating rate assays were performed according to our previous methods [66 (link)].
5
Evaluating CM and PEDF-VEGF Effects on Angiogenesis
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To determine the effect of CM on angiogenesis, the ex ovo chorioallantoic membrane (CAM) assay was performed. Briefly, fertilized white leghorn chicken (Gallus domesticus L.) eggs (Schropper GmbH, Gloggnitz, Austria) were incubated for 3 days at 37.6 °C and 70–75 % relative humidity (J. Hemel Brutgeräte, Am Buschbach, Germany). Eggs were then opened into plastic weigh boats covered with square Petri dishes and returned to the incubator. On day ten, six on-plants were placed on the CAM vasculature. The on-plants consisted of a silicone ring containing either FTB CM, TTB CM or non-conditioned control medium, each on four different eggs. On day 3, vascularization of the on-plants was scored by a blinded observer using a five partite scale between −2 and +2.
The anti-angiogenic potential of PEDF in combination with VEGF was evaluated. Silicone rings contained either collagen (1 mg/ml) mixed with PEDF (10 ng/ml) or VEGF (25 ng/ml), or both, in DMEM/EBM supplemented with 7.5 % FCS. As control, collagen mixed with medium alone was used. For both settings, vessel sprouting was monitored under a microscope (Olympus stereomicroscope SZX16, Tokyo, Japan) immediately after application of the silicone ring on day 0 and each 24 h for 4 days.
The anti-angiogenic potential of PEDF in combination with VEGF was evaluated. Silicone rings contained either collagen (1 mg/ml) mixed with PEDF (10 ng/ml) or VEGF (25 ng/ml), or both, in DMEM/EBM supplemented with 7.5 % FCS. As control, collagen mixed with medium alone was used. For both settings, vessel sprouting was monitored under a microscope (Olympus stereomicroscope SZX16, Tokyo, Japan) immediately after application of the silicone ring on day 0 and each 24 h for 4 days.
6
Fly Eye and Wing Imaging
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Three-day-old flies were collected and frozen at −80 °C. When taking pictures, flies were unfrozen at room temperature and placed on 1% agarose plate. Light images of eye was taken by OLYMPUS stereo microscope SZX16. Wings were dissected and placed on slide with alcohol/glycerol (1:1) buffer. Light images of wing were taken by OLYMPUS BX51 microscope.
7
Mouse Eye Morphometry Analysis
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Freshly excised mouse eye balls were imaged under a Olympus stereo microscope SZX16 equipped with a Q-imaging camera. The lens was also imaged under dark field conditions to better visualize the lens periphery. From the recorded images, the ocular parameters - lengths of the eye along two orthogonal axes were measured manually using FIJI. For the lens, mean feret diameter from the contour of the lens periphery was measured as an average estimate of the size of the lens.
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