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Raav2 flex rev chr2 tdtomato

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RAAV2-FLEX-rev-ChR2:tdTomato is a recombinant adeno-associated virus (rAAV) construct containing a reversible Cre-dependent channelrhodopsin-2 (ChR2) gene fused to a tdTomato fluorescent protein. This construct allows for Cre-mediated expression of ChR2 for optogenetic manipulation of neuronal activity.

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Psico, by Addgene (2 mentions)

2 protocols using raav2 flex rev chr2 tdtomato

1

Lentiviral Vectors for Genetic Manipulation

Cited 1 time
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Lentiviral vector of Sox2 promoter-driven Hsv-TK and matched control were generated as reported previously34 (link). Lentiviral vector of Bmi1 promoter-driven TK was generated by replacing Sox2 promoter with Bmi1 promoter in the above TK plasmid. Lentiviral vector of Sox2 promoter-driven DTR was generated by replacing TK cDNA with the coding sequence of simian DTR in the plasmid of Sox2 promoter-driven TK. Mouse Rab27a shRNA and scramble shRNA were constructed using the vector pSico (Addgene). Rab27a shRNA-1: GGAGAGGTTTCGTA-GCTTA, Rab27a shRNA-2: GCTTCTGTTCGACCTGACA, scramble shRNA sequence: ATCTCGCTTGGGCGAGAGT. Plasmids of rAAV2-FLEX-rev-ChR2:tdTomato were purchased from Addgene. Lentiviruses were produced by transfecting viral plasmids and packaging plasmids into HEK293T cells, purified via ultra-centrifugation and titrated using p24 ELISA kit as described previously32 (link).
Zhang Y., Kim M.S., Jia B., Yan J., Zuniga-Hertz J.P., Han C, & Cai D. (2017). Hypothalamic stem cells control aging speed partly through exosomal miRNAs. Nature, 548(7665), 52-57.
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2

Lentiviral Vectors for Genetic Manipulation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral vector of Sox2 promoter-driven Hsv-TK and matched control were generated as reported previously34 (link). Lentiviral vector of Bmi1 promoter-driven TK was generated by replacing Sox2 promoter with Bmi1 promoter in the above TK plasmid. Lentiviral vector of Sox2 promoter-driven DTR was generated by replacing TK cDNA with the coding sequence of simian DTR in the plasmid of Sox2 promoter-driven TK. Mouse Rab27a shRNA and scramble shRNA were constructed using the vector pSico (Addgene). Rab27a shRNA-1: GGAGAGGTTTCGTA-GCTTA, Rab27a shRNA-2: GCTTCTGTTCGACCTGACA, scramble shRNA sequence: ATCTCGCTTGGGCGAGAGT. Plasmids of rAAV2-FLEX-rev-ChR2:tdTomato were purchased from Addgene. Lentiviruses were produced by transfecting viral plasmids and packaging plasmids into HEK293T cells, purified via ultra-centrifugation and titrated using p24 ELISA kit as described previously32 (link).
Zhang Y., Kim M.S., Jia B., Yan J., Zuniga-Hertz J.P., Han C, & Cai D. (2017). Hypothalamic stem cells control aging speed partly through exosomal miRNAs. Nature, 548(7665), 52-57.
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